RADIOASSAY OF ERYTHROPOIETIN

促红细胞生成素现在被认为是控制红细胞的激素 制作。这个实验室已经开发出一种放射免疫分析方法，它使用 纯放射性碘标记的促红细胞生成素和AN 兔抗促红细胞生成素抗血清的制备。这是一种分析方法 不仅是正常的循环水平，而且是低迷的水平 在已知会抑制红细胞生成和 与已知的刺激相关的激素水平增加 红血球生成，如出血、缺氧或钴注射。这 放射免疫测定显示与几种生物测定有显著的相关性。 系统。使用这种方法，主要来源的红细胞增多症 可区别于次生源的。临床意义的探讨 这一建议是从一种敏感的诊断价值得出的 放射免疫法测定血中促红细胞生成素水平。临床工作 在这项研究中提出的促红细胞生成素反应受控 最终可能对诊断增加或减少有价值的刺激 分泌能力。高危妊娠中的胎儿低氧血症 通过测量羊水中的激素水平来诊断。在……里面 除人促红细胞生成素外，还有多种动物促红细胞生成素 可用这种化验方法测量。建议的动物工作涉及到这两个方面 促红细胞生成素的产生及其靶向作用机制 纸巾。这种激素在胎儿和新生儿红细胞生成中的作用 将被调查以及生产地点和浓度作为 年龄的作用。其他动物研究关注的是 其他激素对促红细胞生成素合成和释放的影响及其与肾外激素的关系 成年动物体内促红细胞生成素的来源。生产的努力 亲和力更高的抗体将继续存在。单特异性抗体 将被分离出来并用于对这些遗址的形态研究 促红细胞生成素生产。此外，还建议进行与以下方面有关的研究 促红细胞生成素放射受体测定法的建立。一种放射感受器 与放射免疫分析相结合的化验将为 促红细胞生成素检测在临床研究中的重要性。这个 从这次调查中获得的知识将大大增加我们的 对促红细胞生成素生物发生和作用机制的认识 以及这种激素在人类正常和疾病状态下的作用。

Erythropoietin is now accepted as the hormone controlling red blood cell production. A radioimmunoassay has been developed in this laboratory using pure radioiodine labeled erythropoietin and an anti-erythropoietin-antiserum prepared in rabbits. This assays measures not only normal circulating levels, but also depressed levels seen following physiological conditions known to depress erythropoiesis and increased hormone levels associated with stimuli known to increase erythropoiesis such as bleeding, hypoxia or cobalt administration. This radioimmunoassay shows a significant correlation with several bioassay systems. Using this method, polycythemias of primary origin are distinguishable from those of secondary origin. The clinical significance of this proposal is derived from the diagnostic value of a sensitive radioimmunoassay for blood levels of erythropoietin. Clinical work proposed in this study deals with erythropoietin responses to controlled stimuli which may ultimately be of value diagnosing increased or decreased secretory ability. Fetal hypoxemia in high risk pregnancies can be diagnosed by measuring the hormone levels in the amniotic fluid. In addition to human erythropoietin a variety of animal erythropoietins are measurable with this assay. Animal work proposed is concerned with both the production of erythropoietin and the mechanism of action on its target tissues. The role of this hormone in fetal and neonatal erythropoiesis will be investigated as well as site of production and concentrations as a function of age. Other animal studies are concerned with the effects of other hormones on erythropoietin synthesis and release and with extrarenal sources of erythropoietin in adult animals. The effort to produce antibodies with higher affinity will continue. Monospecific antibodies will be isolated and used in morphological studies of the sites of erythropoietin production. Also studies are proposed relating to the development of a radioreceptor assay for erythropoietin. A radioreceptor assay in conjunction with the radioimmunoassay will give a new dimension to the importance of erythropoietin detection in clinical studies. THe knowledge gained from this investigation will add significantly to our understanding of the biogenesis and mechanism of action of erythropoietin and to the role of this hormone in normal and diseased states in humans.