RADIOASSAY OF ERYTHROPOIETIN

促红细胞生成素的放射测定

• 批准号: 3336883
• 负责人: GISELA CLEMONS
• 金额: $ 14.34万
• 依托单位: UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-08-01 至 1987-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3336883
• 关键词: antibody formation; embryo /fetus; erythropoiesis; erythropoietin; human tissue; immunohematology; newborn animals; polycythemia; radioassay; radioimmunoassay; radiotracer; scintillation counter; secretion

Erythropoietin is now accepted as the hormone controlling red blood cell production. A radioimmunoassay has been developed in this laboratory using pure radioiodine labeled erythropoietin and an anti-erythropoietin-antiserum prepared in rabbits. This assays measures not only normal circulating levels, but also depressed levels seen following physiological conditions known to depress erythropoiesis and increased hormone levels associated with stimuli known to increase erythropoiesis such as bleeding, hypoxia or cobalt administration. This radioimmunoassay shows a significant correlation with several bioassay systems. Using this method, polycythemias of primary origin are distinguishable from those of secondary origin. The clinical significance of this proposal is derived from the diagnostic value of a sensitive radioimmunoassay for blood levels of erythropoietin. Clinical work proposed in this study deals with erythropoietin responses to controlled stimuli which may ultimately be of value diagnosing increased or decreased secretory ability. Fetal hypoxemia in high risk pregnancies can be diagnosed by measuring the hormone levels in the amniotic fluid. In addition to human erythropoietin a variety of animal erythropoietins are measurable with this assay. Animal work proposed is concerned with both the production of erythropoietin and the mechanism of action on its target tissues. The role of this hormone in fetal and neonatal erythropoiesis will be investigated as well as site of production and concentrations as a function of age. Other animal studies are concerned with the effects of other hormones on erythropoietin synthesis and release and with extrarenal sources of erythropoietin in adult animals. The effort to produce antibodies with higher affinity will continue. Monospecific antibodies will be isolated and used in morphological studies of the sites of erythropoietin production. Also studies are proposed relating to the development of a radioreceptor assay for erythropoietin. A radioreceptor assay in conjunction with the radioimmunoassay will give a new dimension to the importance of erythropoietin detection in clinical studies. THe knowledge gained from this investigation will add significantly to our understanding of the biogenesis and mechanism of action of erythropoietin and to the role of this hormone in normal and diseased states in humans.

促红细胞生成素现在被认为是控制红细胞的激素 生产。 该实验室已开发出一种放射免疫测定法，使用 纯放射性碘标记的促红细胞生成素和 兔体内制备的抗促红细胞生成素抗血清。 该测定测量 不仅是正常的循环水平，而且还出现了低水平 以下已知会抑制红细胞生成的生理条件和 与已知增加的刺激相关的激素水平增加 红细胞生成，例如出血、缺氧或给予钴。 这 放射免疫测定法与多种生物测定法显示出显着相关性 系统。 使用这种方法，原发性红细胞增多症是 与次要起源的物质区别开来。 临床意义 该提案的提出源自敏感的诊断价值 放射免疫测定血液中促红细胞生成素的水平。 临床工作 本研究中提出的涉及促红细胞生成素对受控的反应 最终可能对诊断增加或减少有价值的刺激 分泌能力。 高危妊娠中胎儿低氧血症可能是 通过测量羊水中的激素水平来诊断。 在 除人类促红细胞生成素外，还有多种动物促红细胞生成素 可通过该测定进行测量。 拟议的动物工作涉及两者 促红细胞生成素的产生及其靶标的作用机制 组织。 这种激素在胎儿和新生儿红细胞生成中的作用 将对其生产地点和浓度进行调查 年龄的函数。 其他动物研究关注的是 其他激素对促红细胞生成素的合成和释放以及肾外 成年动物促红细胞生成素的来源。 生产的努力 具有更高亲和力的抗体将继续存在。 单特异性抗体 将被分离并用于以下位点的形态学研究 促红细胞生成素的产生。 还提出了有关以下方面的研究 开发促红细胞生成素放射性受体测定法。 放射受体 与放射免疫分析相结合将为检测提供新的维度 促红细胞生成素检测在临床研究中的重要性。 这 从这次调查中获得的知识将大大增加我们的知识 了解促红细胞生成素的生物发生和作用机制 以及这种激素在人类正常和患病状态下的作用。