RADIOASSAY OF ERYTHROPOIETIN

促红细胞生成素现在被认为是控制红细胞的激素 生产 本实验室开发了一种放射免疫测定法， 纯放射性碘标记的促红细胞生成素和 在兔中制备的抗促红细胞生成素抗血清。 这一化验措施 不仅是正常的循环水平， 在已知抑制红细胞生成和 与已知增加的刺激相关的激素水平增加 红细胞生成，如出血、缺氧或钴给药。 这 放射免疫分析显示与几种生物测定显著相关 系统. 使用这种方法，原发性红细胞增多症， 区别于那些次要来源。 其临床意义 这一建议是来自诊断价值的敏感 红细胞生成素血液水平的放射免疫测定。 临床工作 在这项研究中提出的处理促红细胞生成素的反应，以控制 最终可能具有诊断增加或减少的价值的刺激 分泌能力 高危妊娠中的胎儿低氧血症可能是 通过测量羊水中的激素水平来诊断。 在 除了人促红细胞生成素之外， 可通过该测定进行测量。 建议的动物工作涉及两个方面 促红细胞生成素的产生及其作用机制 组织中 这种激素在胎儿和新生儿红细胞生成中的作用 将进行调查，以及生产地点和浓度作为一个 年龄的函数。 其他动物研究关注的是 其他激素对促红细胞生成素合成和释放的影响， 促红细胞生成素的来源。 努力生产 具有更高亲和力的抗体将继续存在。 单特异性抗体 将被分离出来，并用于形态学研究的网站， 促红细胞生成素的产生。 此外，还建议进行与 红细胞生成素放射受体测定法的发展。 放射受体 结合放射免疫测定的测定将为 促红细胞生成素检测在临床研究中的重要性。 的 从这次调查中获得的知识将大大增加我们的 了解促红细胞生成素的生物起源和作用机制 以及这种激素在人类正常和疾病状态中的作用。

Erythropoietin is now accepted as the hormone controlling red blood cell production. A radioimmunoassay has been developed in this laboratory using pure radioiodine labeled erythropoietin and an anti-erythropoietin-antiserum prepared in rabbits. This assays measures not only normal circulating levels, but also depressed levels seen following physiological conditions known to depress erythropoiesis and increased hormone levels associated with stimuli known to increase erythropoiesis such as bleeding, hypoxia or cobalt administration. This radioimmunoassay shows a significant correlation with several bioassay systems. Using this method, polycythemias of primary origin are distinguishable from those of secondary origin. The clinical significance of this proposal is derived from the diagnostic value of a sensitive radioimmunoassay for blood levels of erythropoietin. Clinical work proposed in this study deals with erythropoietin responses to controlled stimuli which may ultimately be of value diagnosing increased or decreased secretory ability. Fetal hypoxemia in high risk pregnancies can be diagnosed by measuring the hormone levels in the amniotic fluid. In addition to human erythropoietin a variety of animal erythropoietins are measurable with this assay. Animal work proposed is concerned with both the production of erythropoietin and the mechanism of action on its target tissues. The role of this hormone in fetal and neonatal erythropoiesis will be investigated as well as site of production and concentrations as a function of age. Other animal studies are concerned with the effects of other hormones on erythropoietin synthesis and release and with extrarenal sources of erythropoietin in adult animals. The effort to produce antibodies with higher affinity will continue. Monospecific antibodies will be isolated and used in morphological studies of the sites of erythropoietin production. Also studies are proposed relating to the development of a radioreceptor assay for erythropoietin. A radioreceptor assay in conjunction with the radioimmunoassay will give a new dimension to the importance of erythropoietin detection in clinical studies. THe knowledge gained from this investigation will add significantly to our understanding of the biogenesis and mechanism of action of erythropoietin and to the role of this hormone in normal and diseased states in humans.