RADIOASSAY OF ERYTHROPOIETIN

促红细胞生成素现在被认为是控制红色的激素 血细胞的产生。已开发出一种放射免疫分析方法 本实验室使用纯放射性碘标记的促红细胞生成素和 兔抗促红细胞生成素抗血清的制备。这个化验 不仅测量正常的循环水平，而且还测量抑郁 在已知的抑郁的生理条件下出现的水平 红血球生成和激素水平升高与 已知可以增加红细胞生成的刺激物，如出血， 低氧或钴给药。这项放射免疫分析显示 与体内生物测定显著相关 促红细胞生成素。用此方法治疗原发性红细胞增多症 原产地与次生原产地是可以区分的。这个 这一建议的临床意义源于 一种灵敏的血药浓度检测方法的诊断价值 促红细胞生成素。这项研究中提出的临床工作涉及 糖尿病或糖尿病婴儿的促红细胞生成素反应 高血压母亲。高危妊娠中的胎儿低氧血症 可以通过测量羊水中的激素水平来诊断 流体。除人类促红细胞生成素外，还有多种动物 红细胞生成素是可以用这种方法测定的。畜牧工作 Proposed与EP产生组织的个体发育有关 在胎鼠体内。这种激素在新生儿中的作用 将对红细胞生成情况以及地点(S)进行调查 对贫血和低氧的反应产生和浓度 作为年龄和性别的函数。外源EP的影响将是 对新生兔进行调查，以获得关于 新生的动物对EP的代谢是否与 成年人。这些研究可能在未来的治疗中具有临床价值。 重组蛋白对早产儿贫血的影响 促红细胞生成素。其他动物研究关注的是 其他激素对肾脏促红细胞生成素合成和分泌的影响 成人促红细胞生成素的释放和肾外来源 动物。啮齿动物体内高水平EP的可能作用 唾液腺将被进一步追查。所获得的知识 这些调查将大大增加我们的 对其生物发生和激素调控的认识 促红细胞生成素和这种激素在正常和 人类的疾病状态。

Erythropoietin is now accepted as the hormone controlling red blood cell production. A radioimmunoassay has been developed in this laboratory using pure radioiodine labeled erythropoietin and an anti-erythropoietin-antiserum prepared in rabbits. This assay measures not only normal circulating levels, but also depressed levels seen following physiological conditions known to depress erythropoiesis, and increased hormone levels associated with stimuli known to increase erythropoiesis, such as bleeding, hypoxia or cobalt administration. This radioimmunoassay shows a significant correlation with the in vivo bioassay for erythropoietin. Using this method, polycythemias of primary origin are distinguishable from those of secondary origin. The clinical significance of this proposal is derived from the diagnostic value of a sensitive assay of blood levels of erythropoietin. Clinical work proposed in this study deals with erythropoietin responses in infants born to diabetic or hypertensive mothers. Fetal hypoxemia in high risk pregnancies can be diagnosed by measuring the hormone level in the amniotic fluid. In addition to human erythropoietin a variety of animal erythropoietins are measurable with this assay. Animal work proposed is concerned with the ontogeny of Ep producing tissues in the fetal rat. The role of this hormone in neonatal erythropoiesis will be investigated as well as the site(s) of production and concentrations in response to anemia and hypoxia as a function of age and sex. The effects of exogenous Ep will be investigated in neonatal rabbits in order to gain information on whether the newborn animal metabolizes Ep differently than the adult. These studies may be of future clinical value in treatment of the anemia in premature infants with recombinant erythropoietin. Other animal studies are concerned with the effect of other hormones on renal erythropoietin synthesis and release, and with extrarenal sources of erythropoietin in adult animals. The possible function of high Ep levels found in rodent salivary glands will be further pursued. The knowledge gained from these investigations will add significantly to our understanding of the biogenesis and hormonal control of erythropoietin and to the role of this hormone in normal and diseased states in humans.