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RADIOASSAY OF ERYTHROPOIETIN

促红细胞生成素的放射测定

基本信息

批准号：
3336887
负责人：
GISELA CLEMONS
金额：
$ 17.86万
依托单位：
UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB
依托单位国家：
美国
项目类别：
财政年份：
1978
资助国家：
美国
起止时间：
1978-08-01 至 1993-03-31
项目状态：
已结题

项目摘要

Erythropoietin is now accepted as the hormone controlling red blood cell production. A radioimmunoassay has been developed in this laboratory using pure radioiodine labeled erythropoietin and an anti-erythropoietin-antiserum prepared in rabbits. This assay measures not only normal circulating levels, but also depressed levels seen following physiological conditions known to depress erythropoiesis, and increased hormone levels associated with stimuli known to increase erythropoiesis, such as bleeding, hypoxia or cobalt administration. This radioimmunoassay shows a significant correlation with the in vivo bioassay for erythropoietin. Using this method, polycythemias of primary origin are distinguishable from those of secondary origin. The clinical significance of this proposal is derived from the diagnostic value of a sensitive assay of blood levels of erythropoietin. Clinical work proposed in this study deals with erythropoietin responses in infants born to diabetic or hypertensive mothers. Fetal hypoxemia in high risk pregnancies can be diagnosed by measuring the hormone level in the amniotic fluid. In addition to human erythropoietin a variety of animal erythropoietins are measurable with this assay. Animal work proposed is concerned with the ontogeny of Ep producing tissues in the fetal rat. The role of this hormone in neonatal erythropoiesis will be investigated as well as the site(s) of production and concentrations in response to anemia and hypoxia as a function of age and sex. The effects of exogenous Ep will be investigated in neonatal rabbits in order to gain information on whether the newborn animal metabolizes Ep differently than the adult. These studies may be of future clinical value in treatment of the anemia in premature infants with recombinant erythropoietin. Other animal studies are concerned with the effect of other hormones on renal erythropoietin synthesis and release, and with extrarenal sources of erythropoietin in adult animals. The possible function of high Ep levels found in rodent salivary glands will be further pursued. The knowledge gained from these investigations will add significantly to our understanding of the biogenesis and hormonal control of erythropoietin and to the role of this hormone in normal and diseased states in humans.

促红细胞生成素现在被认为是控制红色的激素血细胞的产生。放射免疫分析法已于该实验室使用纯放射性碘标记的促红细胞生成素和在兔子中制备的抗促红细胞生成素抗血清。本次检测不仅测量正常循环水平，还测量抑郁水平在已知会抑制的生理条件下看到的水平红细胞生成和与相关的激素水平增加已知会增加红细胞生成的刺激，例如出血，缺氧或给予钴。该放射免疫分析显示与体内生物测定显着相关促红细胞生成素。使用这种方法，原发性红细胞增多症起源与次要起源是有区别的。这该提议的临床意义源自血药浓度敏感检测的诊断价值促红细胞生成素。本研究提出的临床工作涉及糖尿病或糖尿病所生婴儿的促红细胞生成素反应高血压妈妈们。高危妊娠中胎儿低氧血症可以通过测量羊膜中的激素水平来诊断体液。除人类促红细胞生成素外还有多种动物促红细胞生成素促红细胞生成素可通过该测定法进行测量。动物工作提出与 Ep 产生组织的个体发育有关在胎鼠中。这种激素在新生儿中的作用将研究红细胞生成以及红细胞生成的位点贫血和缺氧时的产量和浓度作为年龄和性别的函数。外源性 Ep 的作用是对新生兔进行研究以获得以下信息新生动物的 Ep 代谢是否与正常动物不同成人。这些研究可能具有未来治疗的临床价值重组早产儿贫血的研究促红细胞生成素。其他动物研究涉及其他激素对肾促红细胞生成素合成的影响释放，并与成人肾外来源的促红细胞生成素动物。在啮齿动物中发现高 Ep 水平的可能功能将进一步研究唾液腺。获得的知识这些调查将大大增加我们的了解生物发生和激素控制促红细胞生成素以及该激素在正常和人类的疾病状态。