THE ANGIOTENSIN I CONVERTING ENZYME

我们将继续对某些肽的酶学进行研究 激素：血管紧张素I、心钠素、下丘脑 促性腺激素释放激素（GnRH）和GnRH相关 可能抑制催乳素释放的肽（GAP） 激素. 我们的目标是： 1.确定活性中心谷氨酸的催化作用 酸的转化酶和确定的性质， 活动现场。 为了解释底物抑制和 非竞争性抑制 研究这种转变的本质 在切割位点。 2.从其cDNA中测定转化酶的序列。 3.为了纯化和研究心房PDH（最近在本研究中发现）， 实验室）在处理C 心房利钠肽末端。 看看里面是否含有 活性位点谷氨酸。 4.化学测序一个非常小的活性位点肽， 心房PDH和测序约200个氨基酸的 活性位点。 5.为了纯化和研究一种酶（最近在这篇文章中发现的）， 实验室），其处理心房肌的N末端 利钠肽 6.为了纯化GAP释放酶并对其进行研究， 在这个实验室中发现的）与其 生理作用。 7.寻找处理GnRH C末端的酶。

We will continue our studies on the enzymology of certain peptide hormones: Angiotensin I, atrial natriuretic peptides, hypothalmic gonadotropin releasing hormone (GnRH), and GnRH associated peptide (GAP) which may be prolactin releasing inhibiting hormone. Our aims are: 1. To determine the catalytic role of the active site glutamic acid of converting enzyme and to determine the nature of the active site. To explain substrate inhibition and noncompetitive inhibition. To study the nature of the shift in the cleavage site. 2. To sequence converting enzyme from its cDNA. 3. To purify and study atrial PDH (recently discovered in this laboratory) with respect to its role in processing the C termini of atrial natriuretic peptides. To see if it contains an active site glutamic acid. 4. To chemically sequence a very small active site peptide of atrial PDH and to sequence about 200 amino acids of the active site from cDNA. 5. To purify and study an enzyme (recently discovered in this laboratory) that processes the N terminus of atrial natriuretic peptides. 6. To purify and study GAP releasing enzyme (recently discovered in this laboratory) in relationship to its physiological role. 7. To look for enzyme(s) that process the C terminus of GnRH.