THE ANGIOTENSIN I CONVERTING ENZYME
THE ANGIOTENSIN I CONVERTING ENZYME
批准号:
3336777
负责人:
IRWIN B WILSON
金额:
$13.73万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1978
资助国家:
美国
项目状态:
已结题
起止时间:
1978-04-01 至 1992-03-31
关键词:
affinity chromatography atrial natriuretic peptide captopril chlorambucil complementary DNA enzyme structure enzyme substrate fluorescence spectrometry gel electrophoresis glutamates gonadotropin releasing factor high performance liquid chromatography human tissue hypothalamus peptide hormone peptidyl dipeptidase A prolactin releasing /inhibiting factor
中文摘要
我们将继续对某些多肽的酶学研究。
激素:血管紧张素-I、心钠素、下丘脑
促性腺激素释放激素及其相关
可能抑制催乳素释放的多肽(GAP)
荷尔蒙。我们的目标是:
1.测定活性部位谷氨酸的催化作用
转化酶的酸度和性质的测定
活动站点。为了解释底物抑制和
非竞争性抑制。研究这种转变的本质
在卵裂部位。
2.从其cDNA中测序转化酶。
3.纯化和研究房性PDH(新近在本研究中发现
实验室)与其在处理C
心钠素的终末。以查看它是否包含
谷氨酸活性部位的谷氨酸。
4.对一个非常小的活性部位多肽进行化学测序。
并对其约200个氨基酸进行了测序。
活性部位来自于cDNA.
5.提纯和研究一种酶(最近在此发现
实验室)处理心房N端的
利钠肽。
6.GAP释放酶的纯化和研究(最近
在本实验室中发现的)与其
生理作用。
7.寻找处理促性腺激素释放激素C末端的酶(S)。
英文摘要
We will continue our studies on the enzymology of certain peptide
hormones: Angiotensin I, atrial natriuretic peptides, hypothalmic
gonadotropin releasing hormone (GnRH), and GnRH associated
peptide (GAP) which may be prolactin releasing inhibiting
hormone. Our aims are:
1. To determine the catalytic role of the active site glutamic
acid of converting enzyme and to determine the nature of
the active site. To explain substrate inhibition and
noncompetitive inhibition. To study the nature of the shift
in the cleavage site.
2. To sequence converting enzyme from its cDNA.
3. To purify and study atrial PDH (recently discovered in this
laboratory) with respect to its role in processing the C
termini of atrial natriuretic peptides. To see if it contains
an active site glutamic acid.
4. To chemically sequence a very small active site peptide of
atrial PDH and to sequence about 200 amino acids of the
active site from cDNA.
5. To purify and study an enzyme (recently discovered in this
laboratory) that processes the N terminus of atrial
natriuretic peptides.
6. To purify and study GAP releasing enzyme (recently
discovered in this laboratory) in relationship to its
physiological role.
7. To look for enzyme(s) that process the C terminus of GnRH.
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会议论文
ANGIOTENSIN CONVERTING ENZYME
-
批准号:2215547
-
项目类别:
-
资助金额:$12.45万
-
财政年份:1978
-
负责人:IRWIN B WILSON
-
依托单位:
ANGIOTENSIN CONVERTING ENZYME
-
批准号:3336782
-
项目类别:
-
资助金额:$11.57万
-
财政年份:1978
-
负责人:IRWIN B WILSON
-
依托单位:
THE ANGIOTENSIN CONVERTING ENZYME
-
批准号:3336779
-
项目类别:
-
资助金额:$11.55万
-
财政年份:1978
-
负责人:IRWIN B WILSON
-
依托单位:
THE ANGIOTENSIN I CONVERTING ENZYME
-
批准号:3336780
-
项目类别:
-
资助金额:$11.76万
-
财政年份:1978
-
负责人:IRWIN B WILSON
-
依托单位:
ANGIOTENSIN CONVERTING ENZYME
-
批准号:3336781
-
项目类别:
-
资助金额:$12.33万
-
财政年份:1978
-
负责人:IRWIN B WILSON
-
依托单位:
THE ANGIOTENSIN CONVERTING ENZYME
-
批准号:3336778
-
项目类别:
-
资助金额:$11.12万
-
财政年份:1978
-
负责人:IRWIN B WILSON
-
依托单位:
海外基金