THE ANGIOTENSIN CONVERTING ENZYME

我们打算继续研究分子结构动力学， 和血管紧张素I转化酶的机械性质。 的 这项赠款具体目标是： 1)为了获得含有一定量的转化酶的限制肽， 或所有活性位点残基，并对该肽进行测序。 2)研究转化酶的化学机理，建立 是否形成酰基酶中间体。 3)研究活性位点结合口袋亚位点的性质， 底物和抑制剂的动力学研究;研究 底物抑制，对单价阴离子的要求， 氰化物和焦磷酸盐抑制的意义。 4)开发一种底物，可用于活性染色， 转化酶活性非常低。 5)为了继续开发生物特异性亲和配体， 并不断提高酶亲和性的一般方法 层析纯化。 6)确定脑组织中转化酶的存在， 其他组织来源，如脑脊液和羊水。 7)研究转化酶的分子和动力学性质 从不同的来源纯化。

We intend to continue our studies on the molecular, structural, kinetic, and mechanistic properties of the angiotensinI-converting enzyme. The specific aim of this grant are: 1) To obtain the limit peptide of the converting enzyme that contains some or all of the active-site residues and to sequence this peptide. 2) To study the chemical mechanism of the converting enzyme to establish whether an acyl-enzyme intermediate is formed. 3) To study the nature of the active site bindingr pocket subsites by the kinetic study of substrates and inhibitors; to study the phenomenon of substrate inhibition, the requirement for monovalent anions, and the significance of inhibition by cyanide and pyrophosphate. 4) To develop a substrate that can be used for an activity stain to detect very low levels of converting enzyme activity. 5) To continue to develop a biospecific affinity ligand for the converting enzyme and to continue to improve the general method of affinity chromatography purification. 6) To establish the existence of converting enzyme in brain tissue and other tissue sources such as cerebrospinal fluid and aminotic fluid. 7) To study the molecular and kinetic properties of the converting enzyme purified from different sources.