ANGIOTENSIN CONVERTING ENZYME

血管紧张素转换酶

• 批准号: 2215547
• 负责人: IRWIN B WILSON
• 金额: $ 12.45万
• 依托单位: UNIVERSITY OF COLORADO
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-04-01 至 1995-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2215547
• 关键词: affinity chromatography; atrial natriuretic peptide; captopril; chlorambucil; complementary DNA; cow; enzyme inhibitors; enzyme mechanism; enzyme structure; enzyme substrate; fluorescence spectrometry; gel electrophoresis; glutamates; gonadotropin releasing factor; high performance liquid chromatography; human tissue; hypothalamus; peptide hormone; peptidyl dipeptidase A; prolactin releasing /inhibiting factor; protein sequence; substance P

We will continue our studies on the enzymology of certain peptide hormones: Angiotensin I, atrial natriuretic peptides, hypothalmic gonadotropin releasing hormone (GnRH), and GnRH associated peptide (GAP) which may be prolactin releasing inhibiting hormone. Our aims are: 1. To determine the catalytic role of the active site glutamic acid of converting enzyme and to determine the nature of the active site. To explain substrate inhibition and noncompetitive inhibition. To study the nature of the shift in the cleavage site. 2. To sequence converting enzyme from its cDNA. 3. To purify and study atrial PDH (recently discovered in this laboratory) with respect to its role in processing the C termini of atrial natriuretic peptides. To see if it contains an active site glutamic acid. 4. To chemically sequence a very small active site peptide of atrial PDH and to sequence about 200 amino acids of the active site from cDNA. 5. To purify and study an enzyme (recently discovered in this laboratory) that processes the N terminus of atrial natriuretic peptides. 6. To purify and study GAP releasing enzyme (recently discovered in this laboratory) in relationship to its physiological role. 7. To look for enzyme(s) that process the C terminus of GnRH.

我们将继续对某些多肽的酶学研究。 激素：血管紧张素-I、心钠素、下丘脑 促性腺激素释放激素及其相关 可能抑制催乳素释放的多肽(GAP) 荷尔蒙。我们的目标是： 1.测定活性部位谷氨酸的催化作用 转化酶的酸度和性质的测定 活动站点。为了解释底物抑制和 非竞争性抑制。研究这种转变的本质 在卵裂部位。 2.从其cDNA中测序转化酶。 3.纯化和研究房性PDH(新近在本研究中发现 实验室)与其在处理C 心钠素的终末。以查看它是否包含 谷氨酸活性部位的谷氨酸。 4.对一个非常小的活性部位多肽进行化学测序。 并对其约200个氨基酸进行了测序。 活性部位来自于cDNA. 5.提纯和研究一种酶(最近在此发现 实验室)处理心房N端的 利钠肽。 6.GAP释放酶的纯化和研究(最近 在本实验室中发现的)与其 生理作用。 7.寻找处理促性腺激素释放激素C末端的酶(S)。

项目成果

Atrial tissue contains a metallo dipeptidyl carboxyhydrolase not present in ventricular tissue: partial purification and characterization.

心房组织含有心室组织中不存在的金属二肽基羧基水解酶：部分纯化和表征。

• DOI: 10.1016/0003-9861(84)90493-4
• 发表时间: 1984
• 期刊: Archives of biochemistry and biophysics
• 影响因子: 3.9
• 作者: Harris,RB;Wilson,IB
• 通讯作者: Wilson,IB

Angiotensin converting enzyme: substrate inhibition.

血管紧张素转换酶：底物抑制。

• DOI: 10.1016/0196-9781(89)90054-5
• 发表时间: 1989
• 期刊: Peptides
• 影响因子: 3
• 作者: Schullek,JR;Wilson,IB
• 通讯作者: Wilson,IB

The binding of zinc to angiotensin-converting enzyme.

锌与血管紧张素转换酶的结合。

• DOI: 10.1016/0003-9861(88)90137-3
• 发表时间: 1988
• 期刊: Archives of biochemistry and biophysics
• 影响因子: 3.9
• 作者: Schullek,JR;Wilson,IB
• 通讯作者: Wilson,IB

tert.-Butyl aminocarbonate (tert.-butyloxycarbonyloxyamine)--a new acylating reagent for amines.

叔丁基氨基碳酸酯（叔丁氧基羰氧基胺）——一种新型胺酰化剂。

• DOI: 10.1111/j.1399-3011.1984.tb02692.x
• 发表时间: 1984
• 期刊: International journal of peptide and protein research
• 作者: Harris,RB;Wilson,IB
• 通讯作者: Wilson,IB

Inhibition and affinity chromatography of human serum angiotensin converting enzyme with cysteinyl-proline derivatives.

半胱氨酰脯氨酸衍生物对人血清血管紧张素转化酶的抑制和亲和层析。

• DOI: 10.1016/0003-9861(81)90071-0
• 发表时间: 1981
• 期刊: Archives of biochemistry and biophysics
• 影响因子: 3.9
• 作者: Harris,RB;Ohlsson,JT;Wilson,IB
• 通讯作者: Wilson,IB