CALCIFICATION IN BLOOD PUMPS

无论如何，血泵钙化是一种常见的并发症。 材料和动物种类。 泵的确切发病机理 钙化是未知的。 在过去的8年里，我们研究了 现象在超过50泵内衬光滑明胶，粗糙聚酯 植绒或粉末金属和200个组织瓣膜置换，我们是 是最早发表血泵钙化研究结果的组织之一。 作为这些研究的结果，我们假设， 心血管植入物，无论是在光滑或粗糙的表面上， 并且无论是在移动区域还是非移动区域上， 机制 其机制是继发性营养不良性钙化 退化细胞存款。 该项目的具体目的是验证这一假设， 识别和表征钙化的性质和时间过程， 目前广泛使用的光滑聚氨酯（Biomer）表面处理 被其他组织的人用在了血泵上。 目前尚不清楚钙化是否 直接发生在聚合物上或聚合物中，或者如果它与聚合物具有相同的机理， 在其他类型的表面。 在第1阶段，体外研究旨在证明降解的血液 细胞可以是钙化的病灶。 各种退化的血细胞 包埋在明胶层中并暴露于钙化溶液或血浆 生理钙和磷水平。 各种影响 还将测试钙化时的脂质水平。 初始 寻找钙化位点及其与细胞成分的关系 使用组织化学、电子显微镜（EM）、X射线微量分析（EDX）， 电子衍射（艾德）和X射线衍射（XRD）。 定量 使用等离子体灰化进行细胞的钙摄取， 原子吸收分光光度法 在II期体内研究中，12个推送器 将植入带有光滑Biomer表面衬里的板式LVAD， 小牛2周到4个月。 经抛光的泵的表面将 用上面提到的同样的技术来研究。 我们亦建议 扩展我们之前的组织瓣膜钙化研究 阐明血泵钙化的发病机制将有助于 也有利于对组织瓣机制的理解 年轻患者钙化和钙化性动脉炎。

Calcificaton in blood pumps is a frequent complicaton regardless of the material and the animal species. The precise pathogenesis of pump calcification is not known. In the past 8 years we have studied this phenomenon in over 50 pumps lined with smooth gelatin, rough polyester flock or powdered metal and 200 tissue valve implantations, and we were the one of the first groups to publish findings of calcification in blood pumps. As a result of these studies, we hypothesize that calcification of cardiovascular implants, regardless of whether on smooth or rough surfaces, and whether on moving or non-moving regions, occurs with the same mechanism. The mechanism is dystrophic calcification occurring secondarily in degenerating cellular deposit. The specific aim of this project is to verify this hypothesis and to identify and characterize the nature and the time course of the calcifying process on the smooth polyurethane (Biomer) surfaces currently widely used by other groups in blood pumps. It is still not known whether calcificaton occurs directly on or in the polymer or if it is of the same mechanism as seen in other types of surfaces. In Phase 1, in vitro studies are designed to prove that the degraded blood cells can be the nidi for calcification. Various degraded blood cells are embedded in a gelatin layer and exposed to calcifying solution or plasma with physiological calcium and phosphorus levels. Effects of various lipids levels on calcification also will be tested. The initial calcification loci and their relation to cellular elements are searched for using histochemistry, electron microscopy (EM), x-ray microanalysis (EDX), electron diffraction (ED) and x-ray diffraction (XRD). Quantitation of calcium uptake by the cells will be performed using plasma ashing and atomic adsorption spectrophotometry. In Phase II in vivo study, 12 pusher plate type LVADs lined with a smooth Biomer surface will be implanted in calves for 2 weeks to 4 months. The surfaces of the explanted pumps will be studied by the same technique mentioned above. We also propose to expand our prior tissue valve calcification studies. The elucidation of the pathogenesis of calcification in blood pumps will also be beneficial in understading the mechanism of tissue valve calcification in young patients and of calicfying artherosclerosis.