IMMUNOCHEMICAL CHARACTERIZATION OF HUMAN PLASMA LIPOPROT

人类载脂蛋白结构的知识有助于 了解正常和异常的脂质转运和代谢。 最近的研究表明，载脂蛋白（apo）A-1和B密切相关， 与动脉粥样硬化的存在有关。 据了解， 由于氨基酸取代导致apoA-1的结构缺陷将 最终导致冠状动脉疾病（CAD）的发展。 然而，目前尚不清楚是否存在结构缺陷， apo-B可引起临床上显著的疾病。 这项建议是 旨在利用一些免疫化学工具来探测 载脂蛋白和脂蛋白颗粒的取向和结构。 载脂蛋白抗原位点的定义将允许 使用阻断抗体来定位在细胞表面的生理活性位点， 载脂蛋白分子 它还将允许探测可能的 CAD患者的结构差异。 短期目标 目前的研究主要是：a）确定载脂蛋白A-I的抗原结构; B）确定载脂蛋白B的免疫化学性质的差异 冠心病患者和非冠心病患者之间的差异 长期目标是：a） 建立冠心病患者载脂蛋白与血脂的关系 冠状动脉疾病; B）用以下方法分选各种apo B组分： 单克隆抗体并描述其可能的致动脉粥样硬化作用; c） 提供了抗原结构和免疫化学特性， 载脂蛋白和脂蛋白;和d）利用单克隆抗体， 研究脂蛋白结构和功能的探针。

Knowledge of human apolipoprotein structure has contributed to the understanding of normal and abnormal lipid transport and metabolism. Recent studies have shown that apolipoprotein (apo) A-1 and B are closely associated with the presence of atherosclerosis. It is known that a structural defect of apo A-1 due to amino acid substitutions will eventually lead to the development of coronary artery disease (CAD). However, it is not clear at the present time whether structural defects in apo-B can give rise to clinically significant disease. This proposal is designed to utilize a number of immunochemical tools to probe the orientation and structure of apolipoproteins and of lipoprotein particles. The definition of the antigenic sites of apolipoproteins will permit the use of blocking antibodies to map the physiologically active sites on the apolipoprotein molecule. It will also permit the probing of possible structural differences in patients with CAD. Short term goals of the present studies will be to: a) map out the antigenic structure of apo A-I; b) determine the differences in the immunochemical properties of apo B between the patients with and without CAD. Long term goals will be to: a) establish the apolipoprotein and lipid relationship in patients with coronary artery disease; b) sort out the various apo B components with monoclonal antibodies and to delineate their possible atherogenic role; c) provide the antigenic structure and immunochemical properties of apolipoproteins and lipoproteins; and d) utilize monoclonal antibodies as probes to study the structure and function of lipoproteins.