LUNG COLLAGEN CROSSLINKING--BIOSYNTHESIS AND MATURATION

肌腱、骨骼、皮肤、 大鼠的软骨和肺。使用新开发的高效液相色谱技术 还原交联剂和不可还原交联剂的分离和定量 目的是研究不同交联物在体内的周转。 前体-产物关系将在不同的组织中确定 含有不同类型的胶原蛋白。我们将专门测试 假设某些关键的交联链是终极 胶原蛋白的代谢命运--降解或沉积成纤维。成熟 到三官能和四官能产物的双官能交联物的 仔细研究，以确定不可还原交联剂在 成熟过程。这些研究应该会让我们开始了解 大鼠肺组织细胞外基质中胶原沉积的研究 正常动物，并应作为必要的数据库，以允许 继发于这些过程的像差的系统研究 病理生理学和疾病。

Collagen crosslinking will be characterized in tendon, bone, skin, cartilage and lungs of rats. Using newly developed HPLC techniques for separation and quantitation of reduced and of nonreducible crosslinks, we intend to study turnover of different crosslinks in vivo. Precursor-product relationships will be defined in different tissues containing different collagen types. We will specifically test the hypothesis that certain key crosslinks are markers of the ultimate metabolic fate of collagen-degradation or deposition as fibers. Maturation of difunctional crosslinks to tri- and tetrafunctional products will be closely examined to ascertain the role of nonreducible crosslinks in the maturation process. These studies should allow us to begin to understand aspects of collagen deposition in the extracellular matrix of the lung in normal animals, and should serve as the requisite data base to allow systematic study of aberrations in these processes secondary to pathophysiology and disease.