LUNG COLLAGEN CROSSLINKING--BIOSYNTHESIS AND MATURATION

胶原交联将在肌腱、骨、皮肤、 软骨和大鼠肺。 使用新开发的HPLC技术， 分离和定量还原和非还原交联，我们 旨在研究不同交联在体内周转。 前体-产品关系将在不同的组织中定义 含有不同类型的胶原蛋白。 我们将专门测试 假设某些关键的交联是最终的 胶原蛋白的代谢命运-降解或沉积为纤维。 成熟 将双官能交联转化为三官能和四官能产物， 仔细检查，以确定不可还原的交联作用， 成熟过程 这些研究应该让我们开始了解 肺细胞外基质中胶原沉积的方面， 正常动物，并应作为必要的数据库， 对这些过程中的畸变进行系统研究， 病理生理学和疾病。

Collagen crosslinking will be characterized in tendon, bone, skin, cartilage and lungs of rats. Using newly developed HPLC techniques for separation and quantitation of reduced and of nonreducible crosslinks, we intend to study turnover of different crosslinks in vivo. Precursor-product relationships will be defined in different tissues containing different collagen types. We will specifically test the hypothesis that certain key crosslinks are markers of the ultimate metabolic fate of collagen-degradation or deposition as fibers. Maturation of difunctional crosslinks to tri- and tetrafunctional products will be closely examined to ascertain the role of nonreducible crosslinks in the maturation process. These studies should allow us to begin to understand aspects of collagen deposition in the extracellular matrix of the lung in normal animals, and should serve as the requisite data base to allow systematic study of aberrations in these processes secondary to pathophysiology and disease.