MODULATION OF LUNG ENDOTHELIAL PERMEABILITY BY AUTACOIDS

Autacoids 对肺内皮细胞通透性的调节

基本信息

  • 批准号:
    3357790
  • 负责人:
  • 金额:
    $ 11.08万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    1989
  • 资助国家:
    美国
  • 起止时间:
    1989-07-10 至 1994-06-30
  • 项目状态:
    已结题

项目摘要

The measurement of blood-tissue permeability in the lung has proven to be extremely difficult. We have developed a new in vitro method to measure the permeability of cultured endothelial cell layers from pulmonary arteries, veins, and microvessels. The method offers a fresh approach and overcomes many of the difficulties of whole animal methods used to study the increased permeability edema often characteristic of the adult respiratory distress syndrome. Blood-tissue permeability increases are difficult to prove in animal models of increased permeability edema. For example, investigators have such the sheep lung- lymph preparation and stimuli such as histamine, endotoxin, and thrombin as models of lung injury similar to increased permeability edema. However, blood-tissue permeability changes cannot be clearly identified in these animal models, since the lung injury is accompanied by increased intravascular pressures which could also account for the observed increased flux of fluid and solutes. In vitro methods will help identify specific permeability changes induced by agents producing lung injury in animals. Using recent advances in cell culture methods, the permeability characteristics of the endothelial component of the blood-tissue barrier have begun to be investigated. However, these results are being questioned, since these in vitro methods find permeabilities 1000 times greater than in vivo estimates. In preliminary experiments, our in vitro method finds endothelial permeabilities similar to in vivo estimates. This new method utilizes a monolayer of endothelial cells cultured on microcarrier beads as a selective barrier to control the passage of molecular species from the mobile phase of a liquid chromatography column to the stationary phase within the cell covered microcarrier beads. We propose to use this method to clarify the role of the endothelial barrier in blood-tissue permeability and, in addition, measure how the permeability properties are altered by naturally occurring stimuli, i.e. autacoids. This proposal has three main aims: 1) to measure the in vitro intercellular permeability of the pulmonary endothelial cell layer to mannitol and polyethylene glycol; 2) to characterize the permeability of the pulmonary endothelial cell layer with respect to tracer size and charge; and 3) to test the hypothesis that autacoids alter pulmonary endothelial cell permeability by measuring permeability changes produced by three autacoids, which have been described as reversible modulators of blood-tissue permeability in the lung: norepinephrine, thrombin and histamine.
测量肺部的血液组织渗透性

项目成果

期刊论文数量(4)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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Frederick R Haselton其他文献

Frederick R Haselton的其他文献

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{{ truncateString('Frederick R Haselton', 18)}}的其他基金

Urine TB diagnostic by amplicon reconstruction for PCR detection of DNA fragments
通过扩增子重建进行 DNA 片段 PCR 检测诊断尿结核
  • 批准号:
    10385847
  • 财政年份:
    2021
  • 资助金额:
    $ 11.08万
  • 项目类别:
Point-of-Care RT-PCR System to Inform COVID-19 and Respiratory Illness Decisions
护理点 RT-PCR 系统可为 COVID-19 和呼吸道疾病决策提供信息
  • 批准号:
    10688237
  • 财政年份:
    2020
  • 资助金额:
    $ 11.08万
  • 项目类别:
Retinal Imaging of Prognostic Indicators of Atherosclerosis
动脉粥样硬化预后指标的视网膜成像
  • 批准号:
    7573116
  • 财政年份:
    2009
  • 资助金额:
    $ 11.08万
  • 项目类别:
Retinal Imaging of Prognostic Indicators of Atherosclerosis
动脉粥样硬化预后指标的视网膜成像
  • 批准号:
    7787531
  • 财政年份:
    2009
  • 资助金额:
    $ 11.08万
  • 项目类别:
Development of DNA Logic Operations for Viral Diagnostics
用于病毒诊断的 DNA 逻辑运算的开发
  • 批准号:
    7573152
  • 财政年份:
    2009
  • 资助金额:
    $ 11.08万
  • 项目类别:
Development of DNA Logic Operations for Viral Diagnostics
用于病毒诊断的 DNA 逻辑运算的开发
  • 批准号:
    7797468
  • 财政年份:
    2009
  • 资助金额:
    $ 11.08万
  • 项目类别:
Multi-spectral quantum dot-based retinal imaging of molecular expression in vivo
基于多光谱量子点的体内分子表达视网膜成像
  • 批准号:
    7351806
  • 财政年份:
    2007
  • 资助金额:
    $ 11.08万
  • 项目类别:
Multi-spectral quantum dot-based retinal imaging of molecular expression in vivo
基于多光谱量子点的体内分子表达视网膜成像
  • 批准号:
    7583895
  • 财政年份:
    2007
  • 资助金额:
    $ 11.08万
  • 项目类别:
Multi-spectral quantum dot-based retinal imaging of molecular expression in vivo
基于多光谱量子点的体内分子表达视网膜成像
  • 批准号:
    7192344
  • 财政年份:
    2007
  • 资助金额:
    $ 11.08万
  • 项目类别:
Lagrangian detection of biomolecular interactions
生物分子相互作用的拉格朗日检测
  • 批准号:
    6763620
  • 财政年份:
    2004
  • 资助金额:
    $ 11.08万
  • 项目类别:

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