EGF AND TGFA IN DEVELOPING AIRWAY EPITHELIUM
EGF AND TGFA IN DEVELOPING AIRWAY EPITHELIUM
批准号:
3353687
负责人:
MILDRED T STAHLMAN
金额:
$14.66万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1986
资助国家:
美国
项目状态:
已结题
起止时间:
1986-09-30 至 1989-09-29
关键词:
developmental genetics electron microscopy epidermal growth factor gene expression genetic transcription histochemistry /cytochemistry histogenesis human tissue immunochemistry infant human (0-1 year) lung messenger RNA peptide hormone biosynthesis postmortem respiratory distress syndrome of newborn respiratory epithelium transforming growth factors
中文摘要
我们的目标是确定生长因子是否与
英文摘要
Our objective is to determine if growth factors can be related to the
physiology of intraparenchymal airway development and response to injury in
the human lung. In the future, new biologial strategies for the treatment
of newborn lung disease may involve the use of growth factors. Precise
information about the synthesis of growth factors and the availability of
their receptors may contribute to the design of such therapies. The
emphasis of this project is to define the cellular sites of synthesis and
changes in the synthesis of epidermal growth factor (EGF), transforming
growth factor alpha (TGFalpha) and the EGF receptor (EGF-R) during lung
development and the perturbed response to injury seen in bronchopulmonary
dysplasia (BPD). Three major hypotheses will be tested: (1) select cell
populations of the intraparenchymal conducting airways produce EGF,
TGFalpha and EGF-R; (2) EGF, or a similar ligand for EGF-R, exerts
autocrine or paracrine effects contributing to the development (and repair)
of pulmonary epithelium; (3) changes in the production of EGF, or growth
factor ligands for EGF-R, may occur during lung development and BPD. These
hypotheses will be tested with the following specific aims. (1) Changes in
the morphological differentiation of airway epithelium during lung
development will be defined by light and electron microscopy. (2)
Sensitive immunocytochemistry (IC) will be used to identify the specific
cell types containing immunoreactive EGF, TGFalpha and EGF-R. Since
neuroendocrine cells may uptake and store peptide hormones and the EGF:
receptor complex is rapidly internalized and degraded after EGF binding, IC
alone does not establish the sites of synthesis (or lack of synthesis) of
these products. (3) The more direct method of nucleic acid in situ
hybridization histochemistry (IHH) will be used to identify the cellular
sites of transcription of EGF, TGFalpha and EGF-R mRNA. IHH alone does not
establish that the target mRNA is translated and processed to the peptide.
Hence, identification of both the mRNA for a peptide and the immunoreactive
peptide in the same or morphologically identical (and phenotypically
defined) cell increases the probability that this cell is synthesizing the
peptide product. (4) Since EGF may be involved in the repair response, the
sites of synthesis of EGF, TGFalpha and EGF-R will be identified in BPD
lungs. Growth factor gene expression may be under developmental control
and/or altered during the response to injury, therefore (5) relative
changes in the gene expression of EGF, TGFalpha and EGF-R will be defined
by quantitative hybridization studies of lung during normal development and
BPD. (6) Additional studies will identify the sites of and changes in the
synthesis of other promising growth-related peptides. These studies will
determine the in vivo sites of EGF synthesis and provide in vivo evidence
of a physiological role for EGF during lung development and the response to
injury found in BPD.
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会议论文
SCOR--RESPIRATORY DISORDERS IN NEONATES AND CHILDREN
-
批准号:3106391
-
项目类别:
-
资助金额:$121.7万
-
财政年份:1991
-
负责人:MILDRED T STAHLMAN
-
依托单位:
EGF AND TGFA IN DEVELOPING AIRWAY EPITHELIUM
-
批准号:3353689
-
项目类别:
-
资助金额:$15.94万
-
财政年份:1986
-
负责人:MILDRED T STAHLMAN
-
依托单位:
EGF AND TGFA IN DEVELOPING AIRWAY EPITHELIUM
-
批准号:3353690
-
项目类别:
-
资助金额:$15.14万
-
财政年份:1986
-
负责人:MILDRED T STAHLMAN
-
依托单位:
NEWBORN PULMONARY DEVELOPMENT FUNCTION AND DISEASE
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批准号:3540427
-
项目类别:
-
资助金额:$6.96万
-
财政年份:1977
-
负责人:MILDRED T STAHLMAN
-
依托单位:
NEWBORN PULMONARY DEVELOPMENT, FUNCTION AND DISEASE
-
批准号:3540425
-
项目类别:
-
资助金额:$12.19万
-
财政年份:1977
-
负责人:MILDRED T STAHLMAN
-
依托单位:
NEWBORN PULMONARY DEVELOPMENT FUNCTION AND DISEASE
-
批准号:3540428
-
项目类别:
-
资助金额:$8.44万
-
财政年份:1977
-
负责人:MILDRED T STAHLMAN
-
依托单位:
NEWBORN PULMONARY DEVELOPMENT FUNCTION AND DISEASE
-
批准号:3540429
-
项目类别:
-
资助金额:$12.01万
-
财政年份:1977
-
负责人:MILDRED T STAHLMAN
-
依托单位:
NEWBORN PULMONARY DEVELOPMENT, FUNCTION AND DISEASE
-
批准号:3540432
-
项目类别:
-
资助金额:$12.72万
-
财政年份:1977
-
负责人:MILDRED T STAHLMAN
-
依托单位:
NEWBORN PULMONARY DEVELOPMENT, FUNCTION AND DISEASE
-
批准号:3540431
-
项目类别:
-
资助金额:$13.32万
-
财政年份:1977
-
负责人:MILDRED T STAHLMAN
-
依托单位:
NEWBORN PULMONARY DEVELOPMENT, FUNCTION AND DISEASE
-
批准号:3540430
-
项目类别:
-
资助金额:$9.19万
-
财政年份:1977
-
负责人:MILDRED T STAHLMAN
-
依托单位:
SCOR: RESPIRATORY DISORDERS IN NEONATES AND CHILDREN
-
批准号:3106397
-
项目类别:
-
资助金额:$115.11万
-
财政年份:1976
-
负责人:MILDRED T STAHLMAN
-
依托单位:
SCOR: RESPIRATORY DISORDERS IN NEONATES AND CHILDREN
-
批准号:3106396
-
项目类别:
-
资助金额:$117.92万
-
财政年份:1976
-
负责人:MILDRED T STAHLMAN
-
依托单位:
NEWBORN LUNG CENTER (SCOR)
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批准号:3106395
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项目类别:
-
资助金额:$117.31万
-
财政年份:1976
-
负责人:MILDRED T STAHLMAN
-
依托单位:
SCOR: RESPIRATORY DISORDERS IN NEONATES AND CHILDREN
-
批准号:3106399
-
项目类别:
-
资助金额:$102.07万
-
财政年份:1976
-
负责人:MILDRED T STAHLMAN
-
依托单位:
NEWBORN LUNG CENTER (SCOR)
-
批准号:3106394
-
项目类别:
-
资助金额:$112.62万
-
财政年份:1976
-
负责人:MILDRED T STAHLMAN
-
依托单位:
SCOR: RESPIRATORY DISORDERS IN NEONATES AND CHILDREN
-
批准号:3106398
-
项目类别:
-
资助金额:$83.78万
-
财政年份:1976
-
负责人:MILDRED T STAHLMAN
-
依托单位:
SCOR: RESPIRATORY DISORDERS IN NEONATES AND CHILDREN
-
批准号:3106390
-
项目类别:
-
资助金额:$116.57万
-
财政年份:1976
-
负责人:MILDRED T STAHLMAN
-
依托单位:
PULMONARY SURFACTANT ASSOCIATED PROTEINS AND RELATED C-TYPE LECTINS IN THE LUNG
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批准号:3843559
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:MILDRED T STAHLMAN
-
依托单位:
LONG-TERM FOLLOW-UP OF NEONATAL LUNG DISORDER
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批准号:3973238
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:MILDRED T STAHLMAN
-
依托单位:
MORPHOLOGICAL STUDIES ON LUNG PARACRINE CELLS
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批准号:4694942
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:MILDRED T STAHLMAN
-
依托单位:
海外基金