ROLE OF CELLULAR RECOGNITION IN CARDIAC MORPHOGENESIS

目标将是研究特定大分子的作用。 参与细胞识别(细胞-细胞)和识别 心脏形态发生中的细胞外基质成分(cell-ECM)。 将进行以下研究：1)分离和鉴定细胞 参与细胞-细胞和细胞-细胞外基质重组的表面大分子；2) 研制针对这些分离株的多克隆和单克隆抗体 大分子；3)进行生物测定以确定这些分子的作用 心脏发育特定时期的大分子；4)分析 这些细胞表面成分与细胞骨架之间的联系 以及5)检测这些成分的表达。 利用cDNA技术和图像技术在形态发生的特定时期 增强分析。心脏形成的基础是 发育各个阶段特定的细胞-细胞和细胞-细胞外基质相互作用 从胚胎到成年。三者之间复杂的相互作用 心脏的主要细胞类型(肌细胞、成纤维细胞和内皮细胞 细胞)将被检查。首先，将进行细胞黏附试验 胎儿、新生儿和成人的心肌细胞、成纤维细胞和内皮细胞 以确定在认识上的异同 用于纯化细胞外基质成分的细胞类型，如纤维连接蛋白、层粘连蛋白和 各种类型的胶原蛋白。细胞与细胞之间的粘附力也将通过 这些相同的细胞类型。使用免疫学方法，抗体将被 针对细胞表面的纯化提取物制备的，用于：1) 抑制细胞-细胞和/或细胞-细胞外基质相互作用，以及2)纯化 和特定大分子的鉴定(S)。产生抗体 针对细胞表面成分将在不同的时间进行检查 利用重组基因技术和荧光技术进行研究 显微镜。细胞分选在心脏意志形成中的作用 在3种特定细胞表面探针显影后进行检查 主要细胞类型。这些细胞表面特定相互作用 与心脏形态发生有关的具有细胞骨架的成分将是 使用针对细胞表面标志物的抗体和各种不同的 细胞骨架的组成部分。通过对这些角色的调查 我们将尝试进一步阐明细胞-细胞和细胞-细胞外基质受体 心脏形态发生的复杂生化调控。

The objective will be to investigate the role of specific macromolecules involved in the cellular recoginition (cell-cell) and in the recognition of the extracellular matrix components (cell-ECM) in cardiac morphogenesis. Investigations will be conducted to: 1) isolate and characterize cell surface macromolecules involved in cell-cell and cell-ECM recongintion; 2) develop both polyclonal and monoclonal antibodies to these isolated macromolecules; 3) conduct bioassays to determine the role of these macromolecules at specific times of development of the heart; 4) analyse the association between these cell surface components and the cytoskeleton of developing myocytes; and 5) examine the expression of these components at specific periods of morphogenesis using cDNA technology and image enhancement analysis. Fundemental to the formation of the heart are the specific cell-cell and cell-ECM interactions at all stages of development from the embryo to the adult. These complex interactions between the three principal cell types of the heart (myocytes, fibroblasts and endothelial cells) will be examined. Initially, cell adhesion assays will be conducted with fetal, neonate and adult myocytes, fibroblasts and endothelial cells to determine the similarities and differences in the recognition of the cell types for purifies ECM components such as fibronectin, laminin and various types of collagens. Cell-cell adhesion will also be assayed with these same cell types. Using an immunological approach, antibodies will be prepared against purified extracts to the cell surface and used for: 1) inhibition of cell-cell and/or cell-ECM interactions, and 2) purification and identification of the specific macromolecule(s). Antibodies produced against cell surface components will be examined at different times of development using recombinant cDNA technology and by fluorescent microscopy. The role of cell sorting in the formation of of the heart will be examined after the development of specific cell surface probes of the 3 principal cell types. The specific interaction of these cell surface components with cytoskeleton in relation to cardiac morphogenesis will be investigated using antibodies against the cell surface markers and various components of the cytoskeleton. Through these investigations on the role of cell-cell and cell-ECM receptors we will attempt to further elucidate the complex biochemical controls of cardiac morphogenesis.