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DISTRIBUTION OF OXYGEN IN SKELETAL AND CARDIAC MUSCLE

骨骼肌和心肌中的氧气分布

基本信息

批准号：
3352737
负责人：
ALFRED CLARK
金额：
$ 8.75万
依托单位：
UNIVERSITY OF ROCHESTER
依托单位国家：
美国
项目类别：
财政年份：
1986
资助国家：
美国
起止时间：
1986-09-08 至 1990-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3352737
关键词：
capillary dogs erythrocytes exercise membrane model mitochondria myocardial infarction myocardium oxygen transport sarcolemma sickle cell anemia striated muscles tobacco abuse

项目摘要

The long-term goal of this project is to construct a complete, quantitative picture of the transport and distribution of oxygen in skeletal and cardiac muscle for resting states, for exercise states, and for the transitions between them. This goal is to be achieved by combining theoretical results of this project with available experimental results. The theoretical results to be obtained are the specific aims of this project. The analyses to be carried out are divided into two categories: the oxygen transport path from red cell to mitochondrion, and experimental techniques used to measure oxygen distribution. The analyses of the transport path will include the following: oxygen release from red cells; oxygen transport from red cell membrane to sarcolemma; oxygen transport from sarcolemma to cytoplasm; oxygen transport from cytoplasm to mitochondrion; intercellular oxygen transport; time-dependent PO2 variations in rest-work transitions. The experimental situations to be analyzed include cell suspensions, and the freezing of working muscle. In addition, an analysis will be developed which will allow the measurement of the oxygen flux from a single capillary in working muscle. These analyses will be carried out by using a combination of asymptotic methods, such as matched asymptotic expansions, and both finite-difference and finite-element numerical methods. The work proposed here will lead to a better understanding of the factors which determine the distribution of oxygen in muscle. The analyses to be developed for normal tissue can be extended to deal with certain pathological cases, such as (1) oxygen release from sickle cells, (2) compromise of myoglobin-facilitated transport in cardiac muscle of smokers by carbon monoxide, and (3) oxygen delivery to cardiac muscle near an infarct.

本项目的长期目标是构建一个完整的、定量的骨骼和心脏中氧的运输和分布图用于休息状态、运动状态和过渡状态的肌肉他们之间这一目标要通过结合理论成果来实现该项目的现有实验结果。理论取得的成果是该项目的具体目标。的分析要进行的分为两类：氧气运输从红细胞到红细胞的途径，以及用于测量氧气分布。输运路径的分析将包括：红细胞的氧释放;氧运输从红细胞膜到肌膜的氧转运细胞质;氧从细胞质向细胞质的转运;细胞间氧运输;休息-工作转换中的时间依赖性PO 2变化。待分析的实验情况包括细胞悬浮液，以及工作肌肉的冻结。此外，还将进行分析，这将允许测量来自单个毛细管的氧通量在工作的肌肉。这些分析将通过使用渐近方法的组合，例如匹配渐近展开，以及有限差分和有限元数值方法。这里提出的工作将导致更好地理解的因素它决定了氧气在肌肉中的分布。分析将为正常组织开发的可以扩展到处理某些病理情况，例如（1）镰状细胞的氧释放，（2）吸烟者心肌肌红蛋白易化转运的损害通过一氧化碳，和（3）氧气输送到心肌附近的一个梗塞