LONG-TERM REGULATION OF THE MICROCIRCULATION

肾性高血压大鼠的急性研究表明，功能性和 发生结构性变化，导致外围设备数量增加 抵抗。研究表明，早期血管收缩的增加 被结构变化所取代，例如稀疏化 微血管，小动脉缩小，小动脉增加 壁厚。需要进行长期研究来记录这些变化 在高血压的发展过程中，每天都是如此。血管运动是一种 血管阻力和血流调节的重要因素 这在有意识的动物身上研究得最好。这就是这件事的目的 研究检查和记录意识中的所有这些机制 肾性高血压兔采用耳腔制剂。测量结果 将由微血管直径和血管运动通过图像分割制成 闭路电视显微镜，双狭缝红细胞速度 闪光灯电视毛细血管红细胞压积测光技术 显微镜观察和体内定量体视学的微血管密度。 微血管反应将在启动后进行研究 1肾和2肾1夹模型中的高血压直到达到新的稳定 达到状态，大约需要2-6个月。血压测量 血浆肾素活性的血液样本将从慢性肾炎患者中获得 腹主动脉中的导管。在这些基线测量之后 数据，将进行实验，以研究导致的机制 变化，如交感神经的影响， 肾素-血管紧张素系统与肌源性反应 腔内压。在其他研究中，肾动脉将不会被夹住 高血压变不可逆前后的回归研究 结构变化，并看看它们是否在 长期肾性高血压的不可逆转性。这些研究发表在 清醒的兔子将提供信息来评估形态和 高血压和低血压的功能变化，它们的时间尺度， 血液长期调节的原因及功能意义 流。

Acute studies in renal hypertensive rats indicate that functional and structural changes occur that contribute to the increase in peripheral resistance. The studies suggest that an early increase in vasoconstriction is superseded by structural alterations such as rarefaction of microvessels, reduction in arteriolar size, and increases in arteriolar wall thickness. Chronic studies are needed to document these changes on a daily basis during the development of hypertension. Vasomotion is an important contributor to vascular resistance and blood flow regulation which is studied best in conscious animals. It is the purpose of this study to examine and document all of these mechanisms in the conscious renal hypertensive rabbit using the ear chambter preparation. Measurements will be made of microvessel diameters and vasomotion by image splitting closed circuit television microscopy, red cell velocity by the dualslit photometric technique, capillary hematocrit by strobe-flash television microscopy, and microvessel density by in vivo quantitative stereology. Microvascular responses will be studied following the initiation of hypertension in the 1-kidney and 2-kidney-1-clip models until a new steady state is reached, approximately 2-6 months. Blood pressure measurements and blood samples for plasma renin activity will be obtained from a chronic catheter in the abdominal aorta. After these measurements of baseline data, experiments will be performed to study the mechanisms leading to the changes, such as the influence of the sympathetic nerves, the renin-angiotensin system, and myogenic responses to increased and decreased intralumenal pressure. In other studies the renal artery will be unclipped before and after hypertension becomes irreversible to study the regression of the structural changes and to see if they play any role in the irreversibility of long term renal hypertension. These studies in the conscious rabbit will provide the information to assess morphological and functional changes in hypertension and hypotension, their time scale, cause, and functional significance concerning long term regulation of blood flow.