Study of Vasocontractile Protein in Renal Artery and Neural Factors in Renal Hypertension

肾动脉血管收缩蛋白及肾性高血压神经因素的研究

• 批准号: 61570759
• 负责人: NAKADA Teruhiro
• 金额: $ 1.41万
• 依托单位: Department of Urology, Faculty of Medicine, Toyama Medical & Pharmaceutical University.
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-61570759/
• 关键词: Central; norepinephrine; renal artery; two-kidney-one clip renal hypertension collagen; non-collagenous protein; 6-hydroxydopamine; 交感神経; two-kidney; one clip Goldblatt hypertension; hypothalamns; norepinephrine; non-collagenタンパク質; 神経因子

Rats had been given intraventricular injection of 6-hydroxydopamine (6-OHDA) before being a clip to the unilateral renal artery (2K-1C) that caused selective ablation of central noradrenergic neurons. Central catecholamines and the in vivo incorporation of ^3H-prolien into the vascular non-collagen protein were determined in 2K-1C rats in acute hypertensive stage. Hypothalamic norepinephrine (NE) concentration in 2K-1C hypertensive rats was 68% (p 0.001) lower than that in 2K-Sham normotensive rats. The incorporation of ^3H-proline into the non-collagen protein of mesenteric artery in 2K-1C rats was 50.5% (p 0.001) greater than that in 2K-Sham rats. Intraventricular injection of 6-OHDA prevented the hypertension concomitant with the reduction of the proline incorporation into the mesenteric artery (p 0.05). The proline incorporation into this artery in 2K-1C rats treated with hexamethonium. Based on these findings, the increased noncollagen protein synthesis in this artery and low level of hypothalamic NE content appear to participate in the development of 2K-1C hypertension in rats.

在单侧肾动脉（2K-1C）夹闭之前，大鼠脑室内注射6-羟基多巴胺（6-OHDA），导致中枢去甲肾上腺素能神经元的选择性消融。在2K-1C大鼠急性高血压阶段测定了中枢儿茶酚胺和^3H-脯氨酸掺入血管非胶原蛋白的体内情况。2K-1C高血压大鼠的下丘脑去甲肾上腺素（NE）浓度比2K-Sham正常血压大鼠低68%（p 0.001）。2K-1C大鼠肠系膜动脉非胶原蛋白中的^3H-脯氨酸掺入量比2K-Sham大鼠高50.5%（p 0.001）。脑室内注射6-OHDA可预防高血压，同时减少脯氨酸掺入肠系膜动脉（p 0.05）。用六烃季胺处理的2K-1C大鼠中脯氨酸掺入该动脉。根据这些发现，该动脉非胶原蛋白合成增加和下丘脑NE含量降低似乎参与了2K-1C高血压的发生。

项目成果

Yoshikawa, M. and Nakada, T.: "High level of central norepinephrine in hypertensive subjects with impired renal function." Internat.Urol.Nephrol.18. 185-192 (1986)

Yoshikawa, M. 和 Nakada, T.：“肾功能受损的高血压患者中枢去甲肾上腺素水平较高。”

Nakade,T;Katayama,T.: "Urologie edited by A.Jardan" SOCIETIE INTERNATIONALE(Paris,France), 413 (1986)

Nakade,T;Katayama,T.：“A.Jardan 编辑的 Urologie”SOCIETIE INTERNATIONALE（法国巴黎），413 (1986)

Nakada, T., Koike, H. and Katayama, T.: "Evidence for a defect in urinary concentrating ability in primary aldosteronism and its reversal by adrenal surgery." Urol. Internat.42. 295-301 (1987)

Nakada, T.、Koike, H. 和 Katayama, T.：“原发性醛固酮增多症中尿液浓缩能力缺陷及其通过肾上腺手术逆转的证据。”

Yohikawa,M;Nakade,T.: Internat.Urol.Nephrol.18. 185-192 (1986)

Yohikawa，M；Nakade，T.：Internat.Urol.Nephrol.18。

Nakada, T., Koike, H., Akiya, T., Katayama, T. et al.: "Liddle's syndrome, and uncommon form of hyporeninemic hypoaldosteronism: functional and histopathological studies." J. Urol.137. 636-640 (1987)

Nakada, T.、Koike, H.、Akiya, T.、Katayama, T. 等人：“利德尔综合征和低肾素性低醛固酮症的罕见形式：功能和组织病理学研究。”