Study of Vasocontractile Protein in Renal Artery and Neural Factors in Renal Hypertension

在单侧肾动脉（2K-1C）夹闭之前，大鼠脑室内注射6-羟基多巴胺（6-OHDA），导致中枢去甲肾上腺素能神经元的选择性消融。在2K-1C大鼠急性高血压阶段测定了中枢儿茶酚胺和^3H-脯氨酸掺入血管非胶原蛋白的体内情况。2K-1C高血压大鼠的下丘脑去甲肾上腺素（NE）浓度比2K-Sham正常血压大鼠低68%（p 0.001）。2K-1C大鼠肠系膜动脉非胶原蛋白中的^3H-脯氨酸掺入量比2K-Sham大鼠高50.5%（p 0.001）。脑室内注射6-OHDA可预防高血压，同时减少脯氨酸掺入肠系膜动脉（p 0.05）。用六甲铵处理的2K-1C大鼠该动脉的脯氨酸掺入。根据这些发现，该动脉非胶原蛋白合成增加和下丘脑NE含量降低似乎参与了2K-1C高血压的发生。

Rats had been given intraventricular injection of 6-hydroxydopamine (6-OHDA) before being a clip to the unilateral renal artery (2K-1C) that caused selective ablation of central noradrenergic neurons. Central catecholamines and the in vivo incorporation of ^3H-prolien into the vascular non-collagen protein were determined in 2K-1C rats in acute hypertensive stage. Hypothalamic norepinephrine (NE) concentration in 2K-1C hypertensive rats was 68% (p 0.001) lower than that in 2K-Sham normotensive rats. The incorporation of ^3H-proline into the non-collagen protein of mesenteric artery in 2K-1C rats was 50.5% (p 0.001) greater than that in 2K-Sham rats. Intraventricular injection of 6-OHDA prevented the hypertension concomitant with the reduction of the proline incorporation into the mesenteric artery (p 0.05). The proline incorporation into this artery in 2K-1C rats treated with hexamethonium. Based on these findings, the increased noncollagen protein synthesis in this artery and low level of hypothalamic NE content appear to participate in the development of 2K-1C hypertension in rats.