Study of Vasocontractile Protein in Renal Artery and Neural Factors in Renal Hypertension

在将6-羟多巴胺（6-OHDA）夹入单侧肾动脉（2K-1C）之前，给大鼠脑室内注射6-羟多巴胺（6-OHDA），导致中枢去甲肾上腺素能神经元选择性消融。测定急性高血压期2K-1C大鼠体内中央儿茶酚胺含量及^ 3h -脯氨酸在血管非胶原蛋白中的掺入。2K-1C高血压大鼠下丘脑去甲肾上腺素（NE）浓度比2K-1C正常大鼠低68% （p < 0.001）。^ 3h -脯氨酸在2K-1C大鼠肠系膜动脉非胶原蛋白中的掺入量比2K-Sham大鼠高50.5% （p 0.001）。脑室内注射6-OHDA可预防高血压并减少脯氨酸进入肠系膜动脉（p < 0.05）。经六甲溴铵治疗的2K-1C大鼠动脉中脯氨酸的掺入。基于这些发现，该动脉中非胶原蛋白合成的增加和下丘脑NE含量的低水平似乎参与了大鼠2K-1C高血压的发展。

Rats had been given intraventricular injection of 6-hydroxydopamine (6-OHDA) before being a clip to the unilateral renal artery (2K-1C) that caused selective ablation of central noradrenergic neurons. Central catecholamines and the in vivo incorporation of ^3H-prolien into the vascular non-collagen protein were determined in 2K-1C rats in acute hypertensive stage. Hypothalamic norepinephrine (NE) concentration in 2K-1C hypertensive rats was 68% (p 0.001) lower than that in 2K-Sham normotensive rats. The incorporation of ^3H-proline into the non-collagen protein of mesenteric artery in 2K-1C rats was 50.5% (p 0.001) greater than that in 2K-Sham rats. Intraventricular injection of 6-OHDA prevented the hypertension concomitant with the reduction of the proline incorporation into the mesenteric artery (p 0.05). The proline incorporation into this artery in 2K-1C rats treated with hexamethonium. Based on these findings, the increased noncollagen protein synthesis in this artery and low level of hypothalamic NE content appear to participate in the development of 2K-1C hypertension in rats.