ELECTROSHOCK--NEUROBIOLOGY AND DRUG INTERACTION

建议的研究旨在提供一些理解的 电休克行为效应的神经化学机制 通过研究不同EST之间的关系， 参数，导致EEG癫痫发作，以及一些长期变化， 神经化学相关。 血清素和多巴胺的转换率， 将研究脑脊液（CSF）中的电解质转运， 清醒兔子的完整大脑， 脑室灌注和同位素稀释技术。 灌注 实验将在EST前和EST后1、7和14天进行。 一系列重复的EST。 释放到灌注液中的儿茶酚胺将 也被研究。 将进行单独的实验，以研究长期 EST对神经递质和丙咪嗪受体结合的影响， 在几个大脑区域中神经递质的神经元摄取。 此外，本发明还提供了一种方法， 将研究药物与EST的相互作用与EEG癫痫发作的关系 和神经化学变化：初步研究将进行与 锂、丙咪嗪、卡巴氮平和氟哌啶醇。

The proposed research is directed to provide some comprehension of the neurochemical mechanisms involved in the behavioral effects of electroshock treatment (EST) by studying the relationships between different EST parameters, resulting EEG seizures, and long-term changes in several neurochemical correlates. Turnover rates of serotonin, and dopamine and electrolyte transport in the cerebrospinal fluid (CSF) will be studied in the intact brain of awake rabbits by a method which combines cerebroventricular perfusion and isotope dilution techniques. Perfusion experiments will be conducted before EST and 1, 7, and 14 days after a series of repeated EST. Release of catecholamines into the perfusate will also be studied. Separate experiments will be conducted to study long-term effects of EST on neurotransmitters and imipramine receptor binding and on neuronal uptake of neurotransmitters in several brain areas. In addition, drug interaction with EST will be studied in relation to the EEG seizure and the neurochemical changes: Initial studies will be conducted with Lithium, Imipramine, Carbamezapine and Haloperidol.