ELECTROSHOCK--NEUROBIOLOGY AND DRUG INTERACTION

这项拟议的研究旨在提供对 电击行为效应的神经化学机制 治疗(EST)--研究不同EST之间的关系 参数，由此导致的脑电发作，以及几个 神经化学相关。5-羟色胺和多巴胺的周转率 脑脊液(CSF)中的电解质转运将在 用一种结合的方法获得清醒兔的完整脑 脑室灌注和同位素稀释技术。灌流 实验将在美国东部夏令时之前以及之后1、7和14天进行 一系列重复的EST。将儿茶酚胺释放到灌流液中 也有待研究。将进行单独的实验来研究长期的 EST对神经递质和丙咪嗪受体结合的影响 神经元对几个脑区神经递质的摄取。此外, 药物与EST的相互作用将与EEG发作的关系进行研究 以及神经化学变化：初步研究将与 锂、丙咪嗪、碳氮平和氟哌啶醇。

The proposed research is directed to provide some comprehension of the neurochemical mechanisms involved in the behavioral effects of electroshock treatment (EST) by studying the relationships between different EST parameters, resulting EEG seizures, and long-term changes in several neurochemical correlates. Turnover rates of serotonin, and dopamine and electrolyte transport in the cerebrospinal fluid (CSF) will be studied in the intact brain of awake rabbits by a method which combines cerebroventricular perfusion and isotope dilution techniques. Perfusion experiments will be conducted before EST and 1, 7, and 14 days after a series of repeated EST. Release of catecholamines into the perfusate will also be studied. Separate experiments will be conducted to study long-term effects of EST on neurotransmitters and imipramine receptor binding and on neuronal uptake of neurotransmitters in several brain areas. In addition, drug interaction with EST will be studied in relation to the EEG seizure and the neurochemical changes: Initial studies will be conducted with Lithium, Imipramine, Carbamezapine and Haloperidol.