Stem Cell Expansion in a Fluidised Bed Bioreactor for Accelerated Osseointegration of Bone Substitute Material

流化床生物反应器中的干细胞扩增加速骨替代材料的骨整合

• 批准号: EP/I015922/1
• 负责人: Marianne Ellis
• 金额: $ 46.62万
• 依托单位: University of Bath
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2011
• 资助国家: 英国
• 起止时间: 2011 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FI015922%2F1
• 关键词: Stem; Cell; Expansion; Fluidised; Bed

Maintenance of an active lifestyle has many health benefits for society, but one drawback is an increase in the number of joint replacements and subsequent revisions of these, plus other orthopaedic interventions, such as bone grafts in complicated fractures or dental procedures, that become necessary with increasing longevity. There are over 70,000 total hip replacements and 73,000 total knee replacements carried out in the UK each year. The current demographics indicate an increasing aging population. In addition to this, joint replacements are being carried out on younger patients the number of replacements and therefore revisions will continue to rise.The gold standard material used in revision of joint replacements is allograft (bone from other hip replacements), but there are growing concerns with its use, such as disease transmission. The gold standard for bone grafts is autograft (bone from the patient), but harvesting it is not without risks and can result in pain at the donor site some years post-operatively. In all cases a limitation is the availability of grafting material. Synthetic ceramic materials are commercially available. Development of a graft material which is a combination of a synthetic ceramic material with a cell population to accelerate the healing process has great potential, with application in all areas where bone substitute is needed for repair and replacement, and if reached will result in improved post-operative quality of life, health and wellbeing. This proposal will develop technology to provide a synthetic bone graft material containing a population of bone-forming cells (stem cells from bone) to improve the osseointegration (the process of bone cells populating the bone graft and integrating it with the patient's own bone). The technology is a fluidised bed bioreactor to culture the stem cells on highly porous ceramic particles. The fluidised bed bioreactor will be designed to provide the ideal environment to obtain particles containing a maximum population of stem cells. Experimental and computational research will be carried out to provide the operating information for the bioreactor to ensure the quality of the stem cell population in the packing material.

保持积极的生活方式对社会有许多健康益处，但一个缺点是关节置换和随后的修复数量增加，以及其他骨科干预措施，例如复杂骨折或牙科手术中的骨移植，随着寿命的延长，这些干预措施变得必要。英国每年进行超过 70,000 例全髋关节置换术和 73,000 例全膝关节置换术。当前的人口统计数据表明人口老龄化不断加剧。除此之外，关节置换术正在对年轻患者进行，因此置换术和翻修术的数量将继续增加。关节置换术翻修术中使用的黄金标准材料是同种异体移植物（来自其他髋关节置换术的骨头），但人们越来越担心其使用，例如疾病传播。骨移植的黄金标准是自体移植（来自患者的骨头），但获取它并非没有风险，并且可能会在术后几年导致供体部位疼痛。在所有情况下，移植材料的可用性都是一个限制。合成陶瓷材料是可商购的。开发一种将合成陶瓷材料与细胞群相结合的移植材料，以加速愈合过程，具有巨大的潜力，可应用于需要骨替代物进行修复和替换的所有领域，如果达到这一目标，将改善术后的生活质量、健康和福祉。该提案将开发技术，提供一种含有骨形成细胞群（来自骨骼的干细胞）的合成骨移植材料，以改善骨整合（骨细胞填充骨移植物并将其与患者自身骨骼整合的过程）。该技术是一种流化床生物反应器，用于在高度多孔的陶瓷颗粒上培养干细胞。流化床生物反应器的设计旨在提供理想的环境，以获得含有最大干细胞群的颗粒。将进行实验和计算研究，为生物反应器提供运行信息，以确保包装材料中干细胞群的质量。