RECEPTOR SIGNALING IN POLYPLOID VASCULAR SMOOTH MUSCLE
RECEPTOR SIGNALING IN POLYPLOID VASCULAR SMOOTH MUSCLE
批准号:
3358740
负责人:
TOMMY A BROCK
金额:
$9.04万
依托单位国家:
美国
项目类别:
财政年份:
1989
资助国家:
美国
项目状态:
已结题
起止时间:
1989-07-01 至 1993-04-30
关键词:
acidity /alkalinity angiotensin II calcium metabolism cardiovascular pharmacology cell cell interaction cytogenetics diploidy flow cytometry fluorescence microscopy gene expression hypertension laboratory rat membrane potentials muscle cells platelet derived growth factor polyploidy potassium chloride receptor sensitivity spontaneous hypertensive rat tissue /cell culture vascular smooth muscle
中文摘要
提出的研究计划侧重于离子,生化和
英文摘要
The proposed research program focuses on ionic, biochemical and
cellular mechanisms of vasoconstrictor agonist (angiotensin II,
KC1) and growth factor (platelet-derived growth factor) action on
diploid and polyploid vascular smooth muscle cells (VSMC)
isolated from different blood vessels (aorta, caudal artery and
mesenteric artery) of normotensive, spontaneously hypertensive
and DOCA/salt hypertensive rats. Freshly isolated, as well as
cultured VSMC will be used in these studies. A detailed analysis
of cytosolic calcium and pH will be made using flow cytometry
and newly developed multiparameter analytical methods related
to fluorescence-ratio imaging microscopy. The specific aims
include: 1) providing a detailed cell-by-cell analysis of resting
cytosolic calcium and pH, as well as membrane potential, in
diploid/tetraploid VSMC via flow cytometry. In addition,
differences in the spatial and regional distribution (nuclear vs.
cytosolic) of cellular calcium and pH will be assessed; 2)
determining whether diploid/polyploid (tetraploid, octaploid)
VSMC from hypertensive animals possess altered signal
transduction pathways as compared to normal diploid VSMC. We
will also compare and contrast the regional differences in
cytosolic calcium and pH following angiotensin II and PDGF
stimulation; 3) evaluating the physiological relevance of cell-cell
interactions between diploid and polyploid populations of VSMC;
and 4) examining the cytogenetic mechanisms which contribute to
the development of the polyploid VSMC phenotype. These studies
should contribute to our basic understanding of the mechanisms of
normal and altered VSMC responsiveness related to the
pathogenesis of hypertension, atherosclerosis and arterial
vasospasm.
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科研奖励(0)
会议论文
CORE--TISSUE CULTURE
-
批准号:6296870
-
项目类别:
-
资助金额:$1.2万
-
财政年份:1996
-
负责人:TOMMY A BROCK
-
依托单位:
OXIDANTS, CALCIUM ION, AND ENDOTHELIUM FUNCTION IN ATHEROSCLEROSIS
-
批准号:6110175
-
项目类别:
-
资助金额:$1.2万
-
财政年份:1996
-
负责人:TOMMY A BROCK
-
依托单位:
CORE--TISSUE CULTURE
-
批准号:6110176
-
项目类别:
-
资助金额:$1.2万
-
财政年份:1996
-
负责人:TOMMY A BROCK
-
依托单位:
OXIDANTS, CALCIUM ION, AND ENDOTHELIUM FUNCTION IN ATHEROSCLEROSIS
-
批准号:6296869
-
项目类别:
-
资助金额:$1.2万
-
财政年份:1996
-
负责人:TOMMY A BROCK
-
依托单位:
VSM CHLCRIDE PERMEABILITY IN HYPERTENTION
-
批准号:3364073
-
项目类别:
-
资助金额:$19.89万
-
财政年份:1990
-
负责人:TOMMY A BROCK
-
依托单位:
VSM CHLORIDE PERMEABILITY IN HYPERTENSION
-
批准号:3364070
-
项目类别:
-
资助金额:$14.51万
-
财政年份:1990
-
负责人:TOMMY A BROCK
-
依托单位:
VSM CHLCRIDE PERMEABILITY IN HYPERTENTION
-
批准号:3364074
-
项目类别:
-
资助金额:$11.72万
-
财政年份:1990
-
负责人:TOMMY A BROCK
-
依托单位:
VSM CHLCRIDE PERMEABILITY IN HYPERTENTION
-
批准号:2221951
-
项目类别:
-
资助金额:$20.6万
-
财政年份:1990
-
负责人:TOMMY A BROCK
-
依托单位:
VSM CHLORIDE PERMEABILITY IN HYPERTENSION
-
批准号:3364072
-
项目类别:
-
资助金额:$2.63万
-
财政年份:1990
-
负责人:TOMMY A BROCK
-
依托单位:
VSM CHLORIDE PERMEABILITY IN HYPERTENSION
-
批准号:3364071
-
项目类别:
-
资助金额:$14.04万
-
财政年份:1990
-
负责人:TOMMY A BROCK
-
依托单位:
RECEPTOR SIGNALING IN POLYPLOID VASCULAR SMOOTH MUSCLE
-
批准号:3358738
-
项目类别:
-
资助金额:$11.48万
-
财政年份:1989
-
负责人:TOMMY A BROCK
-
依托单位:
RECEPTOR SIGNALING IN POLYPLOID VASCULAR SMOOTH MUSCLE
-
批准号:3358737
-
项目类别:
-
资助金额:$11.26万
-
财政年份:1989
-
负责人:TOMMY A BROCK
-
依托单位:
RECEPTOR SIGNALING IN POLYPLOID VASCULAR SMOOTH MUSCLE
-
批准号:3358735
-
项目类别:
-
资助金额:$15.92万
-
财政年份:1988
-
负责人:TOMMY A BROCK
-
依托单位:
RECEPTOR SIGNALING IN POLYPLOID VASCULAR SMOOTH MUSCLE
-
批准号:3358736
-
项目类别:
-
资助金额:$3.33万
-
财政年份:1988
-
负责人:TOMMY A BROCK
-
依托单位:
MECHANISMS OF AGEPC ACTION ON VASCULAR ENDOTHELIUM
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批准号:3351633
-
项目类别:
-
资助金额:$11.93万
-
财政年份:1985
-
负责人:TOMMY A BROCK
-
依托单位:
MECHANISMS OF AGEPC ACTION ON VASCULAR ENDOTHELIUM
-
批准号:3351632
-
项目类别:
-
资助金额:$11.58万
-
财政年份:1985
-
负责人:TOMMY A BROCK
-
依托单位:
MECHANISMS OF AGEPC ACTION ON VASCULAR ENDOHELIUM
-
批准号:3351630
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项目类别:
-
资助金额:$13.38万
-
财政年份:1985
-
负责人:TOMMY A BROCK
-
依托单位:
海外基金