权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

ANALYSIS OF PLASMA LIPOPROTEINS BY 1H NMR SPECTROSCOPY

通过 1H NMR 光谱分析血浆脂蛋白

基本信息

批准号：
3361816
负责人：
JAMES D OTVOS
金额：
$ 6.71万
依托单位：
NORTH CAROLINA STATE UNIVERSITY RALEIGH
依托单位国家：
美国
项目类别：
财政年份：
1990
资助国家：
美国
起止时间：
1990-04-01 至 1993-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3361816
关键词：
angiography apolipoproteins artery blood lipoprotein cholesterol coronary disorder density gradient ultracentrifugation diagnosis quality /standard early diagnosis heart disorder diagnosis high density lipoproteins human subject low density lipoprotein nuclear magnetic resonance spectroscopy protein structure questionnaires rapid diagnosis very low density lipoprotein

项目摘要

The proposed research is aimed at developing and evaluating the clinical utility of new methodology using H1 NMR spectroscopy to quantitate the levels of the constituent lipoproteins of human blood plasma (VLDL, LDL, HDL). The accurate identification of individuals at risk for coronary heart disease (CHD) is a high priority in the national effort to combat this disease. Markers of CHD risk that are in current widespread use for population screening have proven to be of great value in this effort. These include total cholesterol and triglyceride, LDL- and HDL- cholesterol, and apolipoproteins A-1 and B. Accurate measurement of these constituents is a prerequisite for their effective clinical use and continues to be a challenging problem. Our preliminary studies indicate that it is possible in a simple and rapid process to extract the concentrations of VLDL, LDL, and HDL present in an individual's blood from a computer lineshape analysis of the lipid resonances in a H1 NMR spectrum of the plasma. There are still a great number of questions remaining concerning the precise meaning of the data provided by the technique, its accuracy and reproducibility, and its possible clinical uses. The proposed research addresses these questions and has the following as its specific goals; 1) documentation of the NMR spectral relationships between the lipoprotein subspecies that comprise the three broad components (VLDL, LDL, HDL) used in the lineshape analysis, 2) determination of the extent to which variability in subspecies and lipid composition affect the lineshapes and amplitudes at a given temperature of the individual lipoprotein components, 3) optimization of the analytical protocol for NMR plasma lineshape analysis by documenting the influence of factors such as magnetic field strength, temperature, and fitting algorithm on the accuracy of the analysis, 4) determination of the relationships between NMR-derived lipoprotein levels and those provided by lipoprotein lipid and apolipoprotein assays, and 5) evaluation of the potential diagnostic utility of NMR-derived lipoprotein levels by determining their association with CHD as assessed by coronary arteriography.

该研究旨在开发和评估临床使用H1 NMR光谱法的新方法的实用性，人血浆的组成脂蛋白（VLDL，LDL， HDL）。准确识别冠心病风险个体心脏病（CHD）是国家防治工作的重中之重。这种疾病。目前广泛使用的冠心病风险标志物，人口普查已证明在这项工作中具有很大价值。这些包括总胆固醇和甘油三酯，LDL-和HDL- 胆固醇和载脂蛋白A-1和B。精确测量这些成分是其有效临床使用的先决条件，仍然是一个具有挑战性的问题。我们的初步研究表明有可能在一个简单而快速的过程中提取 VLDL，LDL和HDL的浓度存在于个体的血液中，核磁共振波谱中脂类共振的计算机线形分析的血浆。仍然存在大量的问题关于该技术提供的数据的确切含义，准确性和可重复性，以及其可能的临床用途。的建议的研究解决这些问题，并有以下作为其具体目标; 1）记录NMR光谱之间的关系，包括三种主要组分的脂蛋白亚种（VLDL， LDL，HDL）用于线形分析，2）测定亚种和脂质组成的变异性影响在个体的给定温度下的线形和振幅脂蛋白组分，3）NMR分析方案的优化等离子体线形分析通过记录因素的影响，磁场强度、温度和拟合算法分析的准确性，4）确定 NMR衍生的脂蛋白水平和脂蛋白脂质和载脂蛋白测定，和5）评价潜在的诊断通过确定其相关性，利用NMR衍生的脂蛋白水平冠状动脉造影评估的CHD患者。