BEHAVIOR/HISTOPATHOLOGY OF MODEL OF CEREBRAL ISCHEMIA

脑缺血模型的行为/组织病理学

• 批准号: 3378033
• 负责人: BRUCE Thomas VOLPE
• 金额: $ 16.79万
• 依托单位: WINIFRED MASTERSON BURKE MED RES INST
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-09-01 至 1993-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3378033
• 关键词: brain circulation; brain injury; cerebral ischemia /hypoxia; disease /disorder model; ethology; experimental brain lesion; hippocampus; histopathology; ibotenate; immunocytochemistry; laboratory rat; memory disorders; neural degeneration; neurons; neurotoxins; stereotaxic techniques

Rats subjected to transient forebrain ischemia by the method of four vessel occlusion (4-VO) develop predictable and quantifiable lesions diffusely but also focally in the CA-1 zone of the hippocampus. The distribution and severity of regional hippocampal ischemic neuronal damage is a function of the duration of ischemia. Recent behavioral studies suggest that rats subjected to ischemia show isolated impairments in particular aspects of performance on radial 8 arm maze tasks. Specifically animals with ischemic cerebral damage have impaired "working" memory relative to "reference" memory. We plan to correlate changes in 8 arm maze performance with the extent of morphologic injury, and with alterations in hippocampal blood flow and glucose metabolism. The results from these experiments may provide clues for understanding the relationship between (1) local pathologic neuron damage and alterations in local or distant glucose metabolism and blood flow; and (2) metabolic alteration and behavioral deficit, specifically the glucose metabolism of the CA-zones of the hippocampus and memory for radial 8 arm maze tasks.

四血管法制作大鼠短暂性前脑缺血模型 闭塞（4-VO）发展可预测和可量化的病变弥漫性， 也集中在海马体的CA-1区。 的分布和 局部海马缺血性神经元损伤的严重程度是 缺血的持续时间。 最近的行为学研究表明， 在某些方面表现出孤立的损伤， 在径向8臂迷宫任务上的表现。 特别是缺血性的动物 脑损伤损害了相对于“参考”的“工作”记忆 记忆 我们计划将8臂迷宫表现的变化与 形态学损伤的程度，以及海马血液的变化 血流和葡萄糖代谢。 这些实验的结果可能 为理解（1）地方之间的关系提供线索 病理性神经元损伤和局部或远端葡萄糖的改变 代谢和血流;以及（2）代谢改变和行为 赤字，特别是葡萄糖代谢的CA区的 海马和记忆的放射状8臂迷宫任务。