CAPILLARY ELECTROPHORESIS ANALYSIS OF SINGLE NEURONS

中枢神经系统由大量的细胞和突触组成 它们依赖于正常的微妙和精确的化学平衡 功能。这种化学平衡的破坏是导致 神经系统包括主要的神经精神障碍。然而， 由于中枢神经系统的复杂性，它仍然存在 很难准确地定义神经细胞和 正常或疾病状态下的突触。这样做的长期目标是 研究计划是开发和实施新的仪器设备 极大地提高了微分离能力和超灵敏 神经元的化学成分。毛细管电泳法，免费 区带或凝胶填充，将用于从单一分子分级 神经元、突触、神经元释放物或单个突触小泡。 分析物将通过激光诱导的光学手段进行定量 结合电荷的荧光和激光光热偏转 耦合的探测器设备。可能会执行以下分析 比目前的技术灵敏一百万倍以上。

The central nervous system consists of a vast array of cells and synapses which depend on a delicate and precise chemical balance for normal function. Disruption of this chemical balance underlies diseases of the nervous system including the major neuropsychiatric disorders. However, because of the complexity of the central nervous system it remains difficult to define precisely the molecular content of nerve cells and synapses in normal or disease states. The long-term objective of this research program is to develop and implement new instrumentation with greatly increased capability for micro-separation and ultra-sensitive of the chemical content of neurons. Capillary electrophoresis, either free zone or gel-packed, will be used to fractionate molecules from single neurons, synapses, neuronal releasates, or individual synaptic vesicles. Analytes will be quantitated by optical means using laser induced fluorescence and laser opto-thermal deflection in conjunction with charge coupled detector devices. It may be possible to perform analyses which are greater than one million fold more sensitive than current techniques.