CAPILLARY ELECTROPHORESIS ANALYSIS OF SINGLE NEURONS

描述（改编自申请人摘要）：了解如何 中枢神经系统的疾病可以破坏整体功能，这是必要的 来研究神经元之间化学连接的本质。 人类大脑中可能有1万亿个神经元共同发挥作用 通过10（15）个突触，以及许多神经元假设 独特的身份需要化学分析， 细胞间的差异分子组成测定 在细胞和亚细胞水平上的研究对于细胞的发育至关重要 神经细胞在正常情况下 沟通以及这些机制如何在疾病状态下变得有缺陷。 这项研究计划的长期目标是利用毛细管 电泳与高灵敏度荧光检测进行 单个神经元、静脉曲张、突触、亚细胞 隔舱，并释放。目前，现场仪表是 能够分离和检测少至1700个分析物分子 从大规模样品制备中获得。 分析分钟真实的- 然而，世界样本对样本有严格的限制， 操纵 为了便于定量微尺度分析， 荧光标记程序必须适用于柱上标记 天然非荧光分析物。

DESCRIPTION (Adapted from applicant's abstract):To understand how diseases of the CNS can disrupt functions on the whole, it is imperative to investigate the nature of chemical connectivity between neurons. Perhaps one trillion neurons within the human brain function together through 10(15) synapses, and the probability that many neurons assume unique identities demands chemical analyses that are sensitive to variations from cell to cell. Determination of the molecular composition at the cellular and sub-cellular level is critical to development of an accurate model of the mechanisms by which nerve cells normally communicate and how these mechanisms can become flawed in disease states. The long-term objective of this research program is to exploit capillary electrophoresis with high sensitivity fluorescence detection to perform chemical analyses of single neurons, varicosities, synapses, subcellular compartments, and releasates. Presently, on-site instrumentation is capable of fractionating and detecting as few as 1700 analyte molecules taken from a large scale sample preparation. Analysis of minute real- world samples, however, places stringent limitations on sample manipulations. To facilitate quantitative microscale analyses, fluorescent-tagging procedures must be adapted for on-column labeling of natively non-fluorescent analytes.