CELL BIOLOGY OF THE SYNAPSE AND SPECIFIC BRAIN NUCLEI

突触和特定脑核的细胞生物学

• 批准号: 3396532
• 负责人: ROCHELLE S. COHEN
• 金额: $ 8.54万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 1981
• 资助国家: 美国
• 起止时间: 1981-12-01 至 1990-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3396532
• 关键词: actins; adenosinetriphosphatase; axon; brain cell; cell biology; cerebrospinal fluid; cytoskeleton; dendrites; electron microscopy; growth cones; histochemistry /cytochemistry; immunochemistry; laboratory rabbit; laboratory rat; microfilaments; microtubules; myosins; neural information processing; neurogenesis; synapses; synaptic vesicles; synaptosomes

The main objective of this proposal is to determine the locale of cytoskeletal and associated proteins at the developing synapse and in developing neuronal processes, including dendritic spines, dendrites, and axons in the central nervous system. This problem will be addressed in two ways. One will be to examine developing synapses and dendritic spines for the presence and disposition of actin, myosin, and/or a calcium-ATPase or calcium, magnesium-ATPase in rat cerebellum. These proteins may function in the formation of synapses and in the extrusion of dendritic spines. The second will be to examine the locale and distribution of cytoskeletal and associated proteins including microtubule-associated proteins, neurofilament proteins, actin, and myosin in dendritic growth cones, developing dendrites, and axons in rat cerebellum. These proteins may regulate the development of specific neuronal processes. These problems will be addressed using immunocytochemical and cytochemical techniques that permit the precise localization of cytoskeletal and associated proteins. Because of the well-established role of cytoskeleral proteins in cell motility and shape changes, it is likely that they also function in the development of neuronal processes, such as dendrites, dendritic spines, and axons. Shape changes in dendritic spines, for example, have also been implicated in physiological and behavioral changes, as well as learning. Abnormal changes in dendritic spine shape have been seen in Alzheimer's disease, Huntington's disease and mental retardation. Cytoskeletal defects in dendrites, specifically associated with microtubule disposition, have been seen in mental retardation. Fundamental studies on the cellular mechanisms involved in the development of neuronal processes in normal brain will contribute to an understanding of the cellular basis of neurobehavioral disorders.

该提议的主要目的是确定 发育中的突触和 在开发包括树突状刺在内的神经元过程时， 树突和中枢神经系统中的轴突。 这个问题 将以两种方式解决。 一个将是检查发展 突触和树突状刺以存在和处置 肌动蛋白，肌球蛋白和/或钙ATPase或钙，镁ATPase 在大鼠小脑中。 这些蛋白质可能在编队中起作用 突触和树突状刺的挤出。 第二个 将检查细胞骨架的位置和分布 相关蛋白质，包括微管相关蛋白， 树突状生长中的神经丝蛋白，肌动蛋白和肌球蛋白 大鼠小脑中的锥，发育的树突和轴突。这些 蛋白质可能调节特定神经元的发展 过程。 这些问题将使用 免疫细胞化学和细胞化学技术允许 细胞骨架和相关蛋白的精确定位。 由于细胞蛋白质蛋白在 细胞运动和形状变化，很可能也很可能 在神经元过程的发展中起作用，例如 树突，树突状刺和轴突。 树突状的形状变化 例如，刺也与生理和 行为改变以及学习。 异常变化 在阿尔茨海默氏病中已经看到树突状脊柱形状， 亨廷顿疾病和智力低下。 细胞骨架缺陷 在树突中，特别与微管处置相关， 在智力降低中已经看到。 关于 涉及神经元发展的细胞机制 正常大脑的过程将有助于理解 神经行为疾病的细胞基础。