STRUCTURE AND ORGANIZATION OF THE CEREBRAL CORTEX

建议继续研究其形态、组织、 视皮层神经元的突触联系和免疫化学 老鼠，猫和猴子 将采用Golgi-EM技术来检查 神经胶质样细胞的形态和突触连接，局部 回路神经元和锥体细胞。 这些研究的结果将是 与使用推定和潜在的抗体获得的数据相关 神经递质，以确定化学定义的 神经元和它们的末梢。 还建议制定 使用抗体组合的技术，以及抗体加 Golgi-EM来确定差异标记的神经元如何相互作用。 虽然长期以来一直认为只有两种形态类型 在大脑皮层的突触，这一观点受到质疑，因为有 可以区分的轴突末梢肯定不止两种 根据它们的突触囊泡的大小。 其旨在 确定到底有多少类型的轴突终端和突触存在， 它们是分布的，如果可以在源之间进行相关性， 它们的形态和发射器的内容。 最后，将使用大鼠视觉皮层来确定其是否包含 规则排列的垂直方向的神经元组， 皮质是一个有图案的矩阵。 目的是更好地定义皮层神经元如何正常相互作用， 在理解这一点之前，不可能理解 发生在改变的状态，以及神经元功能如何被药物改变。

It is proposed to continue studies of the morphology, organization, synaptic connections and immunochemistry of neurons in the visual cortices of rat, cat, and monkey. A Golgi-EM technique will be employed to examine the morphology and synaptic connections of neurogliaform cells, local circuit neurons, and pyramidal cells. The results of such studies will be correlated with data obtained using antibodies to putative and potential neurotransmitters to determine the distribution of chemically defined groups of neurons and their terminals. It is also proposed to develop techniques for using combinations of antibodies, and antibodies plus Golgi-EM to determine how differentially labeled neurons interact. Although it has long been held that there are only two morphological types of synapses in cerebral cortex, this view is questioned, for there are certainly more than two types of axon terminals which can be distinguished on the basis of the sizes of their synaptic vesicles. It is intended to ascertain how many types of axon terminals and synapses really exist, how they are distributed, and if correlations can be made between the sources of the terminals, their morphology and their transmitter content. Finally, rat visual cortex will be used to determine if it contains regularly arranged groups of vertically oriented neurons, which give the cortex a patterned matrix. The goal is to better define how cortical neurons normally interact, for until this is understood it is not possible to appreciate the changes which occur in altered states, and how neuronal function can be altered by drugs.