LOCAL INTERACTIONS AMONG HIPPOCAMPAL NEURONS

随着现代免疫细胞化学和细胞内 电生理技术、局部电路的复杂性 海马体已经变得明显。 虽然海马体相对来说 简单的皮质结构，它被认为是参与复杂的 信息处理任务。 中间神经元类型的多样性， 发射机及其本地电路必须是提供 具有调节初级投射活动的装置的系统 细胞 在本申请中，我们继续研究那些 interneurons和local本地circuits电路. 此外，我们建议发起一项 一系列的研究检查了各种“创伤性”治疗的效果， 海马的中间神经元，以及损害 特定的脆弱细胞群。 这些研究将平行进行，但密切互动， 跟踪. 形态学表征，通过光和电子 n=显微镜/免疫细胞化学技术，将描述位置， 分支模式，以及特定的神经递质含量 大鼠海马CA_1、CA_3和齿状核区的中间神经元群 海马体。 这些研究也将为体外切片提供指导 电生理学研究试图描述 根据其电生理特性， 它们与主要细胞类型的突触相互作用（使用同时 细胞对的细胞内记录）。 中间神经元将 细胞内标记并依次在形态学水平上检查。 将使用相同的形态学和电生理学技术， 评估各种治疗对海马中间神经元的影响， 依赖于中间神经元的回路 缺血、缺氧和刺激 将对完整动物进行治疗，并对其进行检查 形态学上，并从中获得海马切片， 电生理研究 将进行平行治疗 同时记录疑似脆弱的中间神经元。 将开发程序来“保护”脆弱的中间神经元， 这些治疗的效果。 这些研究将提供一个描述性的基础， 海马中的中间神经元和中间神经元局部回路，并将测试 一些关于中间神经元作用的具体假设， 海马体。

With the development of modern immunocytochemical and intracellular electrophysiological techniques, the complexity of local circuitry in hippocampus has become apparent. Although the hippocampus is a relatively simple cortical structure, it is thought to participate in complex information processing tasks. The diversity of interneuron types, their transmitters, and their local circuitry must be key elements in providing the system with the means of modulating the activity of primary projection cells. In the present application, we continue our study of those interneurons and local circuitries. Further, we propose to initiate a series of studies examining the effects of various "traumatic" treatments on the interneurons of hippocampus, and the consequences of damaging specific vulnerable cell populations. The studies will be carried out on parallel, but closely interacting, tracks. Morphological characterization, by light and electron n=microscopic/immunocytochemical techniques, will describe the locations, arborization patterns, and putative neurotransmitter content of specific interneuron populations in the CA1, CA3, and dentate regions of rat hippocampus. These studies will also provide guidance for in vitro slice electrophysiological investigations with will attempt to characterize interneurons on the basis of their electrophysiological properties and their synaptic inter-actions with principal cell types (using simultaneous intracellular recordings of cell pairs). Interneurons will be intracellularly labeled and examined, in turn, at the morphological level. The same morphological and electrophysiological techniques will be used to assess the effects of various treatments on hippocampal interneurons and interneuron-dependent circuitry. Ischemia, anoxia, and stimulation treatments will be delivered to intact animals, which will be examined morphologically, and from which hippocampal slices will be obtained from electrophysiological investigation. Parallel treatments will be carried out in slices while recording from suspected vulnerable interneurons. Procedures will be developed to "protect" vulnerable interneurons from effects of these treatments. These studies will provide a descriptive basis for understanding interneurons and interneuron local circuitry in hippocampus, and will test a number of specific hypotheses regarding interneuron action in hippocampus.