HYPOXIA OF BRAIN TISSUE SLICES

脑组织切片缺氧

• 批准号: 3398710
• 负责人: GEORGE G SOMJEN
• 金额: $ 14.17万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1982
• 资助国家: 美国
• 起止时间: 1982-07-01 至 1992-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3398710
• 关键词: afferent nerve; antidromic impulse; central nervous system; cerebral cortex; cerebral ischemia /hypoxia; chronic brain damage; electrical potential; electrophysiology; fluorescence microscopy; hippocampus; laboratory mouse; laboratory rat; membrane potentials; neural inhibition; neural transmission; neuropharmacology; polarography; potassium; spinal cord; synapses; voltage /patch clamp

The long-range goal of this project is a comprehensive study of the effects of hypoxia on central nervous tissue of mammals. Since the objective is the effect of oxygen deprivation, separated from indirect effects caused by systemic changes, in vitro preparations are used in many experiments. Recordings from the brains of anesthetized rats will be used to compare effects observed in vitro to the responses of brain in situ. Cerebral hypoxia leads to: I. reversible failure of synaptic functions; II. irreversible loss of function; III. delayed deterioration after initial recovery following reoxygenation. A set of hypotheses has been developed, in part from the literature, and in part from the results of past work in our laboratory concerning the mechanism of this sequence of processes. This proposal is for the testing of key elements of the hypothetical hypoxic process. Experiments planned for this budget period fall into six sub-projects: 1.Hypoxic conduction failure in spinal presynaptic terminals in isolated mouse spinal cord. 2.The changes of EPSP, threshold, membrane potential and membrane current in the reversible phase of hypoxia in hippocampal tissue slices. 3.Intracellular recordings during and after hypoxic and normoxic spreading depression (SD) in hippocampal tissue slices. 4.Reversibility of prolonged, K+-induced SD in cerebral cortex and in hippocampus in the brain of anesthetized rats. 5.Comparison of hypoxic changes in CA1, CA3 and in fascia dentata (FD) of the brain of anesthetized rats. 6.Delayed post-hypoxic changes in hippocampal tissue slices.

该项目的长期目标是对影响的全面研究 低氧对哺乳动物中枢神经组织的影响。因为我们的目标是 缺氧的影响，与由以下因素引起的间接影响分开 系统的变化，体外制剂被用于许多实验。 来自麻醉大鼠大脑的录音将用于比较 体外观察对脑原位反应的影响。 脑缺氧导致：1.可逆性突触功能衰竭；2. 不可逆转的功能丧失；III.最初的延迟恶化 复氧后的恢复。已经开发了一套假设， 部分来自文献，部分来自过去工作的结果 我们的实验室对这一系列过程的机制进行了研究。这 建议对假设的关键要素进行测试 进程。在这一预算期间计划的实验分为六个 子项目： 1.大鼠脊髓突触前终末缺氧性传导功能障碍 小鼠脊髓。 2.EPSP、阈值、膜电位和膜电流的变化 在海马区脑片中处于可逆性缺氧期。 3.低氧和常氧扩散过程中和之后的细胞内记录 海马区脑片中的抑郁(SD)。 4.延长的K+诱导的大脑皮层和大脑皮层SD的可逆性 麻醉大鼠脑内的海马体。 5.大鼠CA1、CA3和齿状筋膜(FD)缺氧性变化的比较 麻醉大鼠的大脑。 6.海马区脑片缺氧后延迟性改变。