HYPOXIA OF BRAIN TISSUE SLICES

该项目的长期目标是全面研究 缺氧对哺乳动物中枢神经组织的影响。因为目标是 缺氧的影响，与由以下因素引起的间接影响分开 全身变化，体外制剂用于许多实验。 来自麻醉大鼠大脑的记录将用于比较 在体外观察到的对原位脑反应的影响。 脑缺氧导致：I.突触功能的可逆性失效; II. 不可逆转的功能丧失; III.初始后延迟恶化 复氧后恢复。已经提出了一系列假设， 部分来自文献，部分来自过去工作的结果， 我们的实验室关于这一系列过程的机制。这 建议是测试假设缺氧的关键因素， 过程本预算期间计划进行的试验分为六项 次级项目： 1.脊髓突触前终末的低电位传导障碍 小鼠脊髓 2. EPSP、阈值、膜电位和膜电流的变化 在海马组织切片缺氧的可逆阶段。 3.低氧和常氧扩散期间和之后的细胞内记录 抑郁症（SD）的海马组织切片。 4. K+诱导的SD在大脑皮层和海马中的可逆性 在麻醉大鼠的大脑中的海马。 5.缺氧时海马CA_1、CA_3区及齿状筋膜（FD）变化的比较 麻醉老鼠的大脑。 6.海马组织切片缺氧后延迟性改变。

The long-range goal of this project is a comprehensive study of the effects of hypoxia on central nervous tissue of mammals. Since the objective is the effect of oxygen deprivation, separated from indirect effects caused by systemic changes, in vitro preparations are used in many experiments. Recordings from the brains of anesthetized rats will be used to compare effects observed in vitro to the responses of brain in situ. Cerebral hypoxia leads to: I. reversible failure of synaptic functions; II. irreversible loss of function; III. delayed deterioration after initial recovery following reoxygenation. A set of hypotheses has been developed, in part from the literature, and in part from the results of past work in our laboratory concerning the mechanism of this sequence of processes. This proposal is for the testing of key elements of the hypothetical hypoxic process. Experiments planned for this budget period fall into six sub-projects: 1.Hypoxic conduction failure in spinal presynaptic terminals in isolated mouse spinal cord. 2.The changes of EPSP, threshold, membrane potential and membrane current in the reversible phase of hypoxia in hippocampal tissue slices. 3.Intracellular recordings during and after hypoxic and normoxic spreading depression (SD) in hippocampal tissue slices. 4.Reversibility of prolonged, K+-induced SD in cerebral cortex and in hippocampus in the brain of anesthetized rats. 5.Comparison of hypoxic changes in CA1, CA3 and in fascia dentata (FD) of the brain of anesthetized rats. 6.Delayed post-hypoxic changes in hippocampal tissue slices.