GENERATION AND REGENERATION OF AXONS

轴突的产生和再生

• 批准号: 3396978
• 负责人: Marion Murray
• 金额: $ 17.99万
• 依托单位: ALLEGHENY UNIVERSITY OF HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 1980
• 资助国家: 美国
• 起止时间: 1980-07-01 至 1992-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3396978
• 关键词: adrenergic receptor; afferent nerve; axon; axon reaction; electron microscopy; fresh water environment; goldfish; histochemistry /cytochemistry; immunochemistry; implant; innervation; laboratory rat; microscopy; nervous system regeneration; neural information processing; neural plasticity; neuroanatomy; neurogenesis; newborn animals; optic nerve; radioassay; retinal ganglion; substance P; superior colliculus; synapses

The first set of proposed experiments is designed to compare and contrast synaptogenesis occurring in a regenerating system, the goldfish retinotectal system, with lesion induced reorganization occurring by collateral sprouting in a non-regenerating system, the rat interpeduncular nucleus (IPN). The specific issues are related to a comparison of the factors regulating reestablishment of specific numbers and patterns of connections by regenerating goldfish retinal axons with the factors regulating collateral sprouting and reactive reinnervation by intact neurons after lesions in mammalian CNS. This information is essential in order to predict the functional consequences of lesion induced synaptogenesis in the adult CNS. A second goal is to determine those cellular processes which distinguish the response of the rat retinal ganglion cell, axotomized and induced to elongate its axon via implantation techniques, from the axotomized but non-regenerating rat retinal ganglion cell and from the regenerating goldfish retinal ganglion cell. These studies should contribute to an elucidation of the processes which account for the robust and directed growth of the axotomized goldfish optic axons and those which account for the normal failure of this growth in the axotomized mammalian central axons.

第一组建议的实验是为了比较和对比 突触发生在再生系统中，金鱼 视网膜顶盖系统，病变引起的重组发生， 侧支发芽在非再生系统，大鼠脚间 核（IPN）。 具体问题涉及比较 调整重建的具体数量和模式的因素 通过再生金鱼视网膜轴突与因子的连接 通过完整的神经调控侧枝发芽和反应性神经再支配 哺乳动物CNS损伤后的神经元。 这一信息对于 为了预测损伤引起的功能后果， 成年人中枢神经系统中的突触发生。 第二个目标是确定那些细胞过程， 切断轴突并诱导的大鼠视网膜神经节细胞的反应 通过植入技术延长其轴突， 非再生大鼠视网膜神经节细胞和来自再生的 金鱼视网膜神经节细胞 这些研究将有助于 阐明了这些过程， 生长的轴突切断金鱼视神经轴突和那些占 这种生长在被切断的哺乳动物中央轴突中的正常失败。