MULTIPLE SITE OPTICAL RECORDING OF MEMBRANE POTENTIAL

膜电位的多位点光学记录

• 批准号: 3397161
• 负责人: BRIAN Matthew SALZBERG
• 金额: $ 22.85万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 1980
• 资助国家: 美国
• 起止时间: 1980-12-01 至 1988-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3397161
• 关键词: action potentials; axon; central nervous system; computer data analysis; dendrites; fluorescent dye /probe; membrane potentials; neuropil; potentiometry; tissue /cell culture

Certain substances, when bound to the membranes of neurons, cardiac and skeletal muscle, salivary acini, and other cells, behave as molecular indicators of membrane potential. The optical properties of these molecules, most notably fluorescence and absorbance, vary in a linear fashion with potential and may, therefore, be used to monitor action potentials, synaptic potentials, or other changes in membrane voltage from a large number of sites at once, without the necessity of using electrodes. We propose to develop more sensitive probes, to extend the technology associated with their use, and to employ these molecular voltmeters for optical recording of membrane potential from hitherto inaccessible regions of single neurons such as axon an neuroendocrine terminals and axonal and dendritic processes and from many sites simultaneously in small assemblages of neurons and in electrical syncitia, in order to study the spatial and temporal patterning of activity. First, we intend to use a computer based system for Multiple Site Optical Recording of Transmembrane Voltage (MSORTV), already constructed and capable of monitoring changes in membrane potential from as many as 124 loci at once, to record patterns of electrical activity throughout syncitia (such as glandular tissue), and to study the properties of truly simple nervous systems -- small artificially constructed ensembles of synaptically connected invertebrate central neurons maintained in culture -- by recording electrical activiy optically from all of their components simultaneously. Second, we propose to use this appratus, with an Argon ion laser light source, to record membrane potential changes from fine processes of single neurons in situ, within an invertebrate neuropil, and isolated in tissue culture. These structures are not penetrable by microelectrodes and are frequently too far away, electrically, for their activity to be reflected in the somata. Finally, we expect to exploit the optical properties of potentiometric probes, and our multiple site optical recording capability, to detect potential changes in vertebrate nerve terminals, and to correlate alterations in the shape of the nerve terminal action potential with the release of neuropeptides.

某些物质，当与心脏和心脏元的膜结合时 骨骼肌，唾液acini和其他细胞的表现为分子 膜电位的指标。 这些的光学特性 分子，最著名的是荧光和吸光度，在线性上有所不同 具有潜力的时尚，因此可以用来监视行动 电势，突触电位或膜电压的其他变化 同时无需使用大量站点 电极。 我们建议开发更敏感的探针，以扩展 与它们使用相关的技术，并采用这些分子 迄今为止膜电位的光学记录的电压计算机 单个神经元（例如神经内分泌）等单个神经元的无法接近区域 终端，轴突和树突过程以及许多地点 同时在神经元的小组合中和电气偏发症中， 为了研究活动的空间和时间模式。 首先，我们打算将基于计算机的系统用于多个网站光学 跨膜电压（MSORTV）的记录，已经构造，并且 能够监测多达124的膜电位变化 立即进行基因座，以记录整个Syncitia的电活动模式 （例如腺组织），并研究真正简单的特性 神经系统 - 突触的人为构造的小型合奏 通过文化中维持的无脊椎动物中央神经元的连接 - 从其所有组件中录制电动性的电动性 同时地。 其次，我们建议使用氩离子激光灯使用此认可 来源，记录膜电位的变化 原位神经元在无脊椎动物神经肽内，并在组织中分离 文化。 这些结构不受微电极的渗透，并且是 经常在电气上太远，无法反映它们的活动 在索马塔。 最后，我们期望利用电位计量的光学特性 探针和我们多个站点的光学记录能力，以检测 脊椎动物神经末端的潜在变化，并相关 与神经终末动作电位形状的改变 神经肽的释放。