Optical Study of Secretion in Mammalian Nerve Terminals

哺乳动物神经末梢分泌物的光学研究

基本信息

  • 批准号:
    6759446
  • 负责人:
  • 金额:
    $ 39.33万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2001
  • 资助国家:
    美国
  • 起止时间:
    2001-07-24 至 2006-06-30
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (provided by applicant): Secretion is one of the most ubiquitous of cellular processes, and the elucidation of its mechanism(s) remains a challenge to cell physiologists. It is becoming increasingly clear, however, that quantal, or vesicular release of neurotransmitters, neuropeptides, neuromodulators, and hormones proceeds differently in different cell types, and that the mammalian neurohypophysis is an excellent model for rapid-release neurosecretory systems including presynaptic terminals. We are now able to monitor, using optical methods having sub-millisecond time resolution, both the electrical events and cytoplasmic calcium changes in nerve terminals of vertebrates, as well as a sequence of intrinsic optical changes (IOC's) that are related directly to the secretory event. Thus, we possess an exceptional array of tools with which to study excitation-secretion (E-S) coupling, and to provide a more complete understanding of synaptic transmission in higher animals, and of the secretory event in general. First, we will use moderate angular resolution light scattering methods, together with fluorescent calcium indicators, voltage sensitive dyes, immuno-gold labeling, electron microscopy, and electron energy loss spectroscopy (EELS) to examine the hypothesis that the triggered release of calcium from intraterminal stores is required for the release of neuropeptides in mammals (and that the stores may be the secretory granules themselves.) We will also examine several alternative explanations for the origin of the IOC's, including the hypothesis that a pH-dependent solubilization of secretory granule contents, mediated by a rise in intraterminal calcium, precedes exocytosis and generates a large and rapid change in light scattering. Second, we will use the neurohypophyses of transgenic mice expressing the pH-sensitive Green Fluorescent Protein, ecliptic-pHluorin, for high time-resolution studies of neuropeptide secretion. Finally, we will characterize extensively the changes in intraterminal calcium during E-S coupling in mammalian nerve terminals, and we will identify the sources and sinks of this critical second messenger, and their specific roles before, during and after exocytosis. These experiments should have a major impact, and should add to our understanding of synaptic transmission, and its failure or malfunction in human neurological disease.
描述(由申请人提供):分泌是最普遍的细胞过程之一,其机制的阐明仍然是一个挑战

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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BRIAN Matthew SALZBERG其他文献

BRIAN Matthew SALZBERG的其他文献

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{{ truncateString('BRIAN Matthew SALZBERG', 18)}}的其他基金

Optical study of secretion in mammalian nerve terminals
哺乳动物神经末梢分泌的光学研究
  • 批准号:
    9004654
  • 财政年份:
    2001
  • 资助金额:
    $ 39.33万
  • 项目类别:
Optical study of secretion in mammalian nerve terminals
哺乳动物神经末梢分泌的光学研究
  • 批准号:
    8295121
  • 财政年份:
    2001
  • 资助金额:
    $ 39.33万
  • 项目类别:
Optical Study of Secretion in Mammalian Nerve Terminals
哺乳动物神经末梢分泌物的光学研究
  • 批准号:
    6400136
  • 财政年份:
    2001
  • 资助金额:
    $ 39.33万
  • 项目类别:
Optical Study of Secretion in Mammalian Nerve Terminals
哺乳动物神经末梢分泌物的光学研究
  • 批准号:
    6540374
  • 财政年份:
    2001
  • 资助金额:
    $ 39.33万
  • 项目类别:
Optical Study of Secretion in Mammalian Nerve Terminals
哺乳动物神经末梢分泌物的光学研究
  • 批准号:
    6612613
  • 财政年份:
    2001
  • 资助金额:
    $ 39.33万
  • 项目类别:
Optical Study of Secretion in Mammalian Nerve Terminals
哺乳动物神经末梢分泌物的光学研究
  • 批准号:
    6917143
  • 财政年份:
    2001
  • 资助金额:
    $ 39.33万
  • 项目类别:
Optical study of secretion in mammalian nerve terminals
哺乳动物神经末梢分泌的光学研究
  • 批准号:
    8611749
  • 财政年份:
    2001
  • 资助金额:
    $ 39.33万
  • 项目类别:
Optical study of secretion in mammalian nerve terminals
哺乳动物神经末梢分泌的光学研究
  • 批准号:
    8431349
  • 财政年份:
    2001
  • 资助金额:
    $ 39.33万
  • 项目类别:
Optical study of secretion in mammalian nerve terminals
哺乳动物神经末梢分泌的光学研究
  • 批准号:
    7895768
  • 财政年份:
    2001
  • 资助金额:
    $ 39.33万
  • 项目类别:
MULTIPLE SITE OPTICAL RECORDING OF MEMBRANE POTENTIAL
膜电位的多位点光学记录
  • 批准号:
    3397167
  • 财政年份:
    1980
  • 资助金额:
    $ 39.33万
  • 项目类别:

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