GABA RECEPTOR CHANNEL OF SPINAL CORD CELL CULTURES

脊髓细胞培养物的 GABA 受体通道

• 批准号: 3399354
• 负责人: MEYER B. JACKSON
• 金额: $ 5.89万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-04-01 至 1986-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3399354
• 关键词: aminobutyrate; barbiturates; benzodiazepines; biophysics; conformation; electrophysiology; membrane permeability; neural plasticity; neuropharmacology; pentobarbital; spinal cord; tissue /cell culture

The patch clamp will be used to record single channel currents coupled to the GABA receptor of mouse spinal cord cell cultures. Data produced by GABA and by the GABA agonists muscimol and pentobarbital will be analyzed by a number of procedures designed to characterize the channel gating mechanism. This basic molecular information will then be used as a framework for the study of the mechanism by which barbiturates and benzodiazepines potentiate the GABA response. Different physiological responses to GABA have previously been identified. An attempt will be made to reduce the observed cellular responses to differences at the level of channel currents, and thereby obtain a biophysical basis for multiple GABA responses. Diversity in the properties of the GABA receptor channel will then be explored in cultures grown under conditions where spontaneous electrical activity is blocked, or where electrical activity is paroxysmal. In response to conditioning by pharmacological treatments which produce altered patterns of electrical activity, neurons may produce GABA receptor channels with different unitary properties and different physiological functions.

膜片钳将被用于记录单通道电流耦合到 小鼠脊髓细胞培养物的GABA受体。 产生的数据 将分析GABA和GABA激动剂蝇蕈醇和戊巴比妥 通过设计用于表征通道选通的多个过程 机制 这些基本的分子信息将被用作 研究巴比妥类药物和 苯二氮卓类增强GABA反应。 不同生理 对GABA的反应以前已经被鉴定。 将尝试 为了减少观察到的细胞对不同水平的细胞反应， 通道电流，从而获得多种GABA的生物物理基础 应答 GABA受体通道特性的多样性将 然后在自然生长的条件下进行探索， 电活动被阻断，或者电活动 突发性的 对药物治疗条件反射的反应 产生改变的电活动模式，神经元可以产生 GABA受体通道具有不同的单一特性和不同的 生理功能。