SYNAPTIC PLASTICITY IN INTERPEDUNCULAR NUCLEUS

脚间核的突触可塑性

• 批准号: 3397215
• 负责人: NICHOLAS J LENN
• 金额: $ 11.93万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 1981
• 资助国家: 美国
• 起止时间: 1981-02-01 至 1987-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3397215
• 关键词: autoradiography; brain mapping; electron microscopy; experimental brain lesion; histochemistry /cytochemistry; mature animal; neural plasticity; newborn animals; synapses

This research program is concerned with specific aspects of the related problems of normal synaptic development in the brain, and how development is modified by brain damage. These anatomical studies utilize the interpeduncular nucleus in rats, a system where specific questions can be answered in detail because of the favorable features known from previous studies. These including separation of specific types of synapses into separate subnuclei, the pairing of one left and one right habenular afferent at 90% of crest synapses, and the location of 95% of S synapses in the ipsilateral half of the central subnucleus in spite of the fact that the axons making these synapses traverse both halves repeatedly. Electron microscopic degeneration will be used to determine the initial distribution of these synapses during development, and whtehter there is subsequent modification of this distribution. These experiments will also determine the developmental process which leads to the left and right synaptic relationships. Horseradish peroxidase will be used to fill and demonstrate the distribution of the synapses formed by individual afferent axons relative to subnuclear boundaries. Also the relation of synapses to the multiple segments of these axons as they recross the interpeduncular nucleus will be determined in normal adult rats, and in neonatally lesioned animals when they have matured. Horseradish peroxidase will also be used to directly determine the relationship of synapse location to subnuclear location of neurons on whose dendrites they occur. The results of these experiments will provide a basis for hypotheses concerning the mechanisms which determine the observed phenomena and concerning possible treatments which would modify these processes. The long term goal is to develop such treatments, and a system on which they can be tested in detail. The use of such treatments would improve the recovery of neurological function in patients suffering from a wide variety of neurological injuries and diseases, ranging from lifelong genetic and acquired diseases to stroke, trauma and the degenerative diseases of the brain and spinal cord at all ages.

该研究计划涉及相关的具体方面 大脑中正常突触发育的问题以及发育如何 因脑损伤而改变。 这些解剖学研究利用 大鼠脚间核，一个可以提出具体问题的系统 由于以前已知的有利功能，因此详细回答了 研究。 其中包括将特定类型的突触分离成 单独的亚核，一个左侧和一个右侧缰核配对 90% 的嵴突触为传入神经，95% 的 S 突触位于 中央亚核的同侧一半，尽管事实上 形成这些突触的轴突反复穿过两半。 电子 微观退化将用于确定初始分布 这些突触在发育过程中的变化，以及是否有后续的 修改此分布。 这些实验也将决定 导致左右突触的发育过程 关系。 将使用辣根过氧化物酶来填充和演示 由单个传入轴突形成的突触的分布 相对于亚核边界。 还有突触与突触的关系 当这些轴突重新穿过脚间时，它们的多个部分 将在正常成年大鼠和新生损伤大鼠中测定细胞核 动物成熟时。 辣根过氧化物酶也将被使用 直接确定突触位置与亚核的关系 它们出现在其树突上的神经元的位置。 这些实验的结果将为假设提供基础 关于决定观察到的现象的机制和 关于可能改变这些过程的治疗方法。 这 长期目标是开发此类治疗方法以及一种系统 可以详细测试一下。 使用此类治疗将改善 患有多种疾病的患者的神经功能恢复 神经损伤和疾病，包括终生遗传和 后天性疾病如中风、创伤和退行性疾病 所有年龄段的大脑和脊髓。