MEMBRANE RECEPTORS FOR SWEETNESS

甜味膜受体

• 批准号: 3563717
• 负责人: GORAN B HELLEKANT
• 金额: $ 16.76万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1981
• 资助国家: 美国
• 起止时间: 1981-05-01 至 1987-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3563717
• 关键词: chemical structure; chemoreceptors; electron microscopy; electrophysiology; histochemistry /cytochemistry; immunochemistry; molecular site; monoclonal antibody; neurophysiology; sensory disorder diagnosis; stimulus /response; sweetening agents; taste; taste disorders

This research proposal is a combined neurophysiological and morphological investigation of the structure of membrane receptors for tastants in primate taste buds. Thaumatin, monellin and miraculin are proteins that in small quantities have very potent, long lasting effects exclusively in man and certain closely related primates. Thaumatin and monellin on a molar basis are both 104-105 times as sweet as sucrose. As little as 0.1 mg of miraculin (which in itself does not taste sweet) can make acids taste as if sugar has been added. Because all available data indicate that the rhesus monkey's response to these proteins is similar to that of man, this animal will be the primary experimental model. In neurophysiological experiments, we shall study in whole nerve and single fiber of the chorda tympani proper and glossopharyngeal nerves the effects of thaumatin, monellin, and derivatives of thaumatin, both alone and in combination with other compounds. Polyclonal and monoclonal antibodies against thaumatin and monellin will be used to explore their mechanism of action on sweet taste. In addition, we shall study the mechanism of the miraculin effect by use of miraculin antibodies. Since these substances have no effect on the sweet taste of rats, this species will be used as control animals. Our goals are to determine the location of binding sites for these proteins, and to relate the binding capacity of the proteins to their sweetness as revealed by physiological and morphological studies. The data may also be useful in understanding the molecular basis of hypogeusia, dysgeusia, and ageusia in man. We feel that our laboratories through our access to rhesus monkeys, our long experience in this area of research, our advanced facilities, our close cooperation with the biochemists who first characterized thaumatin and miraculin and shared in the characterization of monellin, and still are the only supplier of these substances, as well as through our own expertise, offer a unique research opportunity to elucidate the sweet taste in man.

该研究建议是神经生理和形态学的组合 研究味道剂的膜受体结构 灵长类动物的味蕾。 Thaumatin，Monellin和Miraculin是蛋白质 少量在人中具有非常有效的持久效果 和某些密切相关的灵长类动物。 thaumatin和monellin在磨牙上 基础都是蔗糖的104-105倍。 仅为0.1毫克 奇迹蛋白（本身不会味道甜）可以使酸味，好像 添加了糖。 因为所有可用的数据都表明恒河猴 猴子对这些蛋白质的反应类似于人类的动物 将是主要的实验模型。 在神经生理实验中， 我们将在整个神经和单纤维中进行研究 和全咽神经thaumatin，Monellin和 单独和与其他的衍生物 化合物。 抗thaumatin的多克隆和单克隆抗体和单克隆抗体 Monellin将用于探索他们对甜味的作用机理。 此外，我们将通过使用 奇迹蛋白抗体。 由于这些物质对甜味没有影响 味道大鼠，该物种将用作对照动物。 我们的目标是 确定这些蛋白质的结合位点的位置，并 将蛋白质与它们的甜度相关联 通过生理和形态学研究。 数据也可能在 了解缺乏症，增生症和年龄的分子基础 男人。 我们认为，通过进入恒河猴，我们的实验室 在这一研究领域的长期经验，我们的高级设施，我们的 与首先描述thaumatin的生物化学家的密切合作 和奇迹蛋白并在Monellin的特征中共享，仍然是 这些物质的唯一供应商以及我们自己的 专业知识，提供独特的研究机会来阐明甜味 在男人。

项目成果

Labeling of sweet taste binding sites using a colloidal gold-labeled sweet protein, thaumatin.

使用胶体金标记的甜味蛋白索马甜来标记甜味结合位点。

• 发表时间: 1987
• 期刊: Scanning microscopy
• 作者: Farbman,AI;Ogden-Ogle,CK;Hellekant,G;Simmons,SR;Albrecht,RM;vanderWel,H
• 通讯作者: vanderWel,H

Effects of gymnemic acid on the chorda tympani proper nerve responses to sweet, sour, salty and bitter taste stimuli in the chimpanzee.

武靴叶酸对黑猩猩鼓索适当神经对甜、酸、咸和苦味刺激的反应的影响。

• DOI: 10.1111/j.1748-1716.1985.tb07675.x
• 发表时间: 1985
• 期刊: Acta physiologica Scandinavica
• 作者: Hellekant,G;afSegerstad,CH;Roberts,T;vanderWel,H;Brouwer,JN;Glaser,D;Haynes,R;Eichberg,JW
• 通讯作者: Eichberg,JW

Evidence for a novel mechanism of binding and release of stimuli in the primate taste bud.

灵长类味蕾结合和释放刺激的新机制的证据。

• DOI: 10.1523/jneurosci.09-10-03522.1989
• 发表时间: 1989
• 期刊: The Journal of neuroscience : the official journal of the Society for Neuroscience
• 作者: Farbman,AI;Hellekant,G
• 通讯作者: Hellekant,G

The sweet taste in the calf. I. Chorda tympani proper nerve responses to taste stimulation of the tongue.

小牛身上的甜味。

• DOI: 10.1016/0031-9384(89)90084-x
• 发表时间: 1989
• 期刊: Physiology & behavior
• 影响因子: 2.9
• 作者: Segerstad,CH;Hellekant,G
• 通讯作者: Hellekant,G