MULTIPLE SITE OPTICAL RECORDING OF MEMBRANE POTENTIAL

膜电位的多位点光学记录

• 批准号: 3397162
• 负责人: BRIAN Matthew SALZBERG
• 金额: $ 21.78万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 1980
• 资助国家: 美国
• 起止时间: 1980-12-01 至 1988-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3397162
• 关键词: action potentials; axon; central nervous system; computer data analysis; dendrites; fluorescent dye /probe; membrane potentials; neuropil; potentiometry; tissue /cell culture

Certain substances, when bound to the membranes of neurons, cardiac and skeletal muscle, salivary acini, and other cells, behave as molecular indicators of membrane potential. The optical properties of these molecules, most notably fluorescence and absorbance, vary in a linear fashion with potential and may, therefore, be used to monitor action potentials, synaptic potentials, or other changes in membrane voltage from a large number of sites at once, without the necessity of using electrodes. We propose to develop more sensitive probes, to extend the technology associated with their use, and to employ these molecular voltmeters for optical recording of membrane potential from hitherto inaccessible regions of single neurons such as axon an neuroendocrine terminals and axonal and dendritic processes and from many sites simultaneously in small assemblages of neurons and in electrical syncitia, in order to study the spatial and temporal patterning of activity. First, we intend to use a computer based system for Multiple Site Optical Recording of Transmembrane Voltage (MSORTV), already constructed and capable of monitoring changes in membrane potential from as many as 124 loci at once, to record patterns of electrical activity throughout syncitia (such as glandular tissue), and to study the properties of truly simple nervous systems -- small artificially constructed ensembles of synaptically connected invertebrate central neurons maintained in culture -- by recording electrical activiy optically from all of their components simultaneously. Second, we propose to use this appratus, with an Argon ion laser light source, to record membrane potential changes from fine processes of single neurons in situ, within an invertebrate neuropil, and isolated in tissue culture. These structures are not penetrable by microelectrodes and are frequently too far away, electrically, for their activity to be reflected in the somata. Finally, we expect to exploit the optical properties of potentiometric probes, and our multiple site optical recording capability, to detect potential changes in vertebrate nerve terminals, and to correlate alterations in the shape of the nerve terminal action potential with the release of neuropeptides.

某些物质，当与神经元、心脏和神经细胞的膜结合时 骨骼肌、唾液腺泡和其他细胞，表现为分子 膜电位指标。 这些材料的光学特性 分子，尤其是荧光和吸光度，呈线性变化 时尚具有潜力，因此可用于监测行动 电位、突触电位或膜电压的其他变化 一次处理大量站点，无需使用 电极。 我们建议开发更灵敏的探针，以扩展 与其使用相关的技术，并利用这些分子 迄今为止用于光学记录膜电位的电压表 单个神经元的不可到达区域，例如轴突和神经内分泌 终端、轴突和树突状突起，来自许多位点 同时在神经元的小集合和电合胞体中， 以研究活动的空间和时间模式。 首先，我们打算使用基于计算机的多站点光学系统 跨膜电压记录 (MSORTV)，已构建并 能够监测多达 124 个膜电位的变化 一次位点，记录整个合胞体的电活动模式 （例如腺组织），并研究真正简单的特性 神经系统——人工构建的小型突触集合体 在培养物中维持连接的无脊椎动物中枢神经元——通过 以光学方式记录其所有组件的电活动 同时地。 其次，我们建议使用该装置，带有氩离子激光灯 源，记录单个精细过程的膜电位变化 神经元原位，在无脊椎动物神经纤维内，并在组织中分离 文化。 这些结构不能被微电极穿透，并且 通常距离太远（电气），无法反映其活动 在索马塔。 最后，我们希望利用电位计的光学特性 探头和我们的多站点光学记录能力，以检测 脊椎动物神经末梢的潜在变化，并将 神经末梢动作电位形状的改变 神经肽的释放。