IMMUNOGLOBULIN ACCESS TO NEOANTIGENS ON CNS CELLS

定义的抗原特异性免疫球蛋白 同种型可以进入中枢神经系统（CNS），这些 免疫球蛋白可以克服其扩散的潜在限制， 沿着间质途径到达并与表达的抗原相互作用 在神经细胞表面上的作用基本上是未知的 尽管如此，CNS 免疫球蛋白的进入及其与神经细胞的相互作用 新抗原对于目前的几种关于肿瘤发病机制的理论是至关重要的。 中枢神经系统的自身免疫性疾病，包括多发性硬化， 各种CNS疾病的潜在免疫疗法。 本研究将 利用放射自显影和免疫组织化学形态学 方法和批量转移研究，以确定是否，如何和何时 确定的单克隆抗体进入CNS， 新抗原在脑细胞表面的出现。 一个最近 腮腺炎糖蛋白单克隆抗体库的建立 病毒、免疫细胞化学和放射自显影方法， 所描述的仓鼠腮腺炎-脑膜脑炎模型联合收割机形成 实验系统将与这些问题进行探讨。

The circumstances under which antigen specific immunoglobulins of defined isotype can enter the central nervous system (CNS) and whether these immunoglobulins can overcome potential restrictions to their diffusion along interstitial pathways to reach and interact with antigens expressed on the surfaces of neural cells are essentially unknown. Despite this, CNS entry of immunoglobulins and their interaction with neural cells bearing neoantigens is critical to several current theories of the pathogenesis of autoimmune disorders of the CNS including multiple sclerosis and for the potential immunotherapy of a variety of CNS disorders. This study will utilize both autoradiographic and immunohistochemical morphologic approaches, and bulk transfer studies to determine whether, how and when defined monoclonal antibodies gain entry to the CNS in response to the appearance of neoantigens at the surfaces of brain cells. A recently developed library of monoclonal antibodies to the glycoproteins of mumps virus, immunocytochemical and autoradiographic approaches and the well described model of mumps-meningoencephalitis in hamsters combine to form the experimental system with which these problems will be approached.