EXPERIMENTAL CYTOMEGALOVIRAL LABYRINTHITIS

病毒感染被认为在多种疾病中起病因作用。 影响内耳的疾病 特别是巨细胞病毒， 一种被认为是最重要的传染病的病毒 感音神经性听力损失在美国，而病毒涉及 对于这些疾病，关于内分泌的发病机制实际上知之甚少。 耳朵病毒感染 我们已经建立了一个可重复的豚鼠巨细胞病毒模型 耳炎，其中非免疫动物成为聋子和免疫动物 在随后的内耳病毒攻击中保持听力。 通过 一种跨学科的方法，其中免疫学，病毒学， 这种疾病的电生理学、形态学和代谢方面将 进行调查，以下关于这种疾病的基本问题将 1）GPCMV的具体特点是什么 尿道炎？ 2)什么是局部内耳和全身免疫 对GPCMV尿道炎的反应？ 3)什么是生理和 GPCMV的生化效应？ 4)什么治疗措施 可以避免病毒对内耳的伤害吗 通过使用免疫测定， 免疫组织化学光、电镜定位研究 微观水平，过继转移实验，电生理 监测，耳蜗和CNS 2-脱氧葡萄糖和能量分散X射线 分析研究，更好地了解所涉及的基本机制 在病毒性内耳感染的发病机制中应该会出现。

Viral infections are believed to play an etiologic role in multiple disorders which affect the inner ear. Cytomegalovirus, in particular, is one virus which is recognized as being the most important infectious cause of sensorineural hearing loss in the U.S. While viruses are implicated in these disorders, little is actually known about the pathogenesis of inner ear viral infections. We have developed a reproducible guinea pig model of cytomegalovirus labyrinthitis, in which non-immune animals become deaf and immune animals maintain their hearing upon subsequent inner ear viral challenge. Through an interdisciplinary approach in which the immunologic, virologic, electrophysiologic, morphologic and metabolic aspects of this disease will be investigated, the following basic questions regarding this disorder will be addressed: 1) What are the specific characteristics of GPCMV labyrinthitis? 2) What are the local inner ear and systemic immune responses to GPCMV labyrinthitis? 3) What are the physiological and biochemical effects of GPCMV labyrinthitis? 4) What therapeutic measures can avert viral injury in the inner ear? Through the use of immunoassay, immunohistochemical localization studies at the light and electron microscopic level, adoptive transfer experiments, electro-physiological monitoring, and cochlear and CNS 2-deoxyglucose and energy dispersive x-ray analysis studies, a better understanding of the basic mechanisms involved in the pathogenesis of viral inner ear infections should emerge.