PARVOVIRUS ASSOCIATED CRANIOFACIAL DISTURBANCES

细小病毒相关的颅面障碍

• 批准号: 3425083
• 负责人: GEORGE S SCHUSTER
• 金额: $ 2.13万
• 依托单位: AUGUSTA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-03-01 至 1988-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3425083
• 关键词: DNA; Parvoviridae; bone disorder; cell type; face; mucopolysaccharides; radiotracer; skull; virus diseases; virus replication

The long term objectives of these studies are to examine at the molecular level the mechanisms by which rat virus produces cranio-facial disturbances. The specific aims of this pilot project are to examine the ability of the virus to replicate in calvaria of newborn animals; examine the morphological effects of the virus on the bones and determine degree and site of infection; initiate studies on the effects of the virus on extracellular matrix components; and determine if the virus replicates in the different cell types of the growing calvaria. Calvaria will be infected in vitro with virus and the overall ability of the virus to replicate will be examined by isolation and titration. The specific sites where virus are present will be determined histologically and by the use of specific DNA probes. The ability of the cells to produce mucopolysaccharides and collagen will be determined using radioisotope labeling and extraction. The different cell types present in calvaria will be separated and grown in culture and the ability of virus to replicate in them will be measured.

这些研究的长期目标是在分子水平上检查 水平老鼠病毒产生颅面 干扰. 该试点项目的具体目标是审查 病毒在新生动物头盖骨中复制的能力;检查 病毒对骨骼的形态学影响及其程度 和感染部位;开始研究病毒对 细胞外基质成分;并确定病毒是否在 生长中的头盖骨的不同细胞类型。 在体外，颅骨会被病毒感染， 将通过分离和滴定检查待复制的病毒。 的 将通过组织学方法确定病毒存在的具体部位 以及通过使用特异性DNA探针。 细胞产生的能力 将使用放射性同位素测定粘多糖和胶原蛋白 标记和提取。 存在于颅盖骨中的不同细胞类型将 分离并在培养物中生长，病毒在培养物中复制的能力 他们将被衡量。

项目成果

Replication of rat virus in neonatal calvaria in culture.

大鼠病毒在培养的新生儿颅盖中的复制。

• DOI: 10.1007/bf02620879
• 发表时间: 1988
• 期刊: In vitro cellular & developmental biology : journal of the Tissue Culture Association
• 作者: Schuster,GS;O'Dell,NL;Wilson,JT;Ward,DC
• 通讯作者: Ward,DC