Phosphate Bio-mineral-Ultrafast Laser interaction - a pathway for future hard tissue re-engineering (Novel Tool for Surgical technologies) - LUMIN

磷酸盐生物矿物-超快激光相互作用 - 未来硬组织再造的途径（外科技术的新工具） - LUMIN

• 批准号: EP/K020234/1
• 负责人: Animesh Jha
• 金额: $ 127.09万
• 依托单位: University of Leeds
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2013
• 资助国家: 英国
• 起止时间: 2013 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FK020234%2F1
• 关键词: Phosphate; Bio; mineral; Ultrafast; Laser

Acid erosion due to food and drink intake in particular and tooth surface loss due to general wear of the dentition is a global problem. Continual erosion and loss of the surface enamel of the tooth leads to hypersensitivity. This oral condition is acute in both children and the ageing population of society and can have a significant impact on the quality of life. The 2011 census points out that 16.3% of the population of England and N Ireland is above 65 years old (Daily Telegraph 17 July 2012), which suggests that the number of people suffering from acid erosion may continue to rise in years to come. This means that there is an even more urgent need to provide a robust solution for restoring lost enamel, a problem that remains intractable for clinical dentistry. To address this problem, we propose research into an engineering methodology to spray the tooth with a thin mineral layer that is then densified and bonded to the underlying tooth using an ultrafast laser irradiation pulse. The cross-disciplinary LUMIN project will develop and exploit the technology of micro-nozzle bio-mineral delivery in Task (a) and its subsequent sintering using femto-second pulsed (fsp) lasers for the restoration of acid-eroded enamel. The operating wavelength of the proposed fsp lasers will be in the eye-safe regions of the near-IR (1500-2100 nm) and will offer flexibility in terms of energy/power delivery by engineering the laser cavity, which is the main goal of Task (b). An additional goal of Task (b), as stated in the objective section above, is to integrate the micro-nozzle bio-mineral delivery system from Task (a) with lasers on a single platform for achieving rapid sintering in the deposited bio-mineral layers on to the acid-eroded enamel surface. During this research, novel acid-resistant enamel mineral substitutes, in crystalline and gel forms, will be engineered and optimized for the micro-nozzle delivery in Task (a). The integration of the materials delivery system with the fsp-laser will then yield simultaneous sintering.. The engineering approaches herein will therefore yield 3 different platform technologies for future exploitation, which will be achieved with the support from the Integrated Knowledge Centre on Tissue Engineering and Medical Technologies at Leeds. We will investigate whether the use of a micro-nozzle for gel and suspension materials with an fsp-laser poses a risk of toxicity due to generation and release of nano-scale particulates (some may argue these might be photosensitized by the intense beam of the fsp-laser). In Task (c) we will therefore assess any nano-particle and photo-induced toxicity and perform a risk analysis. This will conform to standard clinical procedures with an aim to thus identify and minimise any imminent risk. Following Task (c), our goal in Task (d) is to implement the engineering approaches, developed in Tasks (a) and (b) together with the risk mitigation strategy in Task (c) for testing fsp-laser sintered enamel minerals in the oral environment using in-situ mouth appliance trials, a technique pioneered at the Leeds Dental Institute to minimising the risks in extensive in-vivo trials. In Task (d) the sintered materials will be characterised for acid erosion, durability, hardness, toughness, and flexural bend with using the assembled academic expertise in materials science and engineering and clinical dentistry.The IKC team will provide support, via Dr. Graeme Howling's expertise, to develop technology exploitation through the project partners, M-Squared Lasers, British Glass, and Giltec in the first instance. The project also aims to establish academic links with overseas academic institutions e.g. the IMI at Lehigh and Penn State in Materials Science, and with Stanford and Caltec in the US via the SUPA led EPSRC funded collaboration. The industry-academia link with the Photonics KTN in the UK is also expected to develop during the course of project.

尤其是由于食物和饮料摄入而引起的酸侵蚀，由于牙列的普遍磨损而导致的牙齿表面损失是一个全球问题。牙齿的持续侵蚀和表面搪瓷的丧失导致过敏。这种口腔状况在儿童和社会老龄化的人口中都很急躁，可以对生活质量产生重大影响。 2011年的人口普查指出，英格兰和恩爱尔兰人口中有16.3％的人口超过65岁（2012年7月17日，每日电讯报），这表明酸侵蚀的人数可能会在未来几年继续增加。这意味着，更需要为恢复丢失的搪瓷提供强大的解决方案，这对于临床牙科仍然很棘手。为了解决这个问题，我们建议对工程方法进行研究，以用薄薄的矿物质层喷洒牙齿，然后使用超快激光辐照脉冲将其致密并粘合到下面的牙齿上。跨学科的Lumin项目将在任务（a）中开发和利用微型嘴生物矿物传递的技术及其随后使用FEMTO秒脉冲（FSP）激光器进行烧结以恢复酸性搪瓷。拟议的FSP激光器的工作波长将位于近红外（1500-2100 nm）的眼部安全区域，并通过工程激光腔（B）的主要目标（B）来实现能源/功率传递方面的灵活性。如上所述，任务（b）的另一个目标是将来自任务（a）的微功能性生物矿物质输送系统与单个平台上的激光整合到一个平台上，以实现沉积的生物矿物质层中的快速烧结到酸磨影的搪瓷表面。在这项研究中，将针对任务（a）中的微鼻嘴递送进行设计和优化的新型耐酸搪瓷矿物替代物，以结晶和凝胶形式进行设计和优化。然后，材料输送系统与FSP激光器的整合将同时产生同时烧结。因此，此处的工程方法将产生3种不同的平台技术，以供将来的剥削，这将在利兹组织组织工程和医疗技术的综合知识中心的支持下实现。我们将调查使用微抽油烟的凝胶和带有FSP激光器的悬浮材料是否会产生毒性的风险，这是由于纳米级颗粒的产生和释放而引起的（有些人可能认为这些可能会被FSP激光器的强烈光束吸收敏感）。因此，在任务（c）中，我们将评估任何纳米粒子和光诱导的毒性并进行风险分析。这将符合标准临床程序，以便识别和最大程度地降低任何迫在眉睫的风险。以下任务（c），我们在任务（d）中的目标是在任务（a）和（b）中实施工程方法，以及在（c）中使用降低风险的降低策略（c），用于在口腔内使用静坐设备试验的口服环境中的FSP激光烧结牙釉质矿物，该技术在Leeds的牙科范围内进行试验，以实现牙科的范围，以实现牙科的范围，以实现牙齿的范围，以实现MINMISS INSTINTS，以最大程度地化。 In Task (d) the sintered materials will be characterised for acid erosion, durability, hardness, toughness, and flexural bend with using the assembled academic expertise in materials science and engineering and clinical dentistry.The IKC team will provide support, via Dr. Graeme Howling's expertise, to develop technology exploitation through the project partners, M-Squared Lasers, British Glass, and Giltec in the first instance.该项目还旨在与海外学术机构建立学术联系，例如Lehigh和Penn State的IMI在材料科学领域，以及在美国通过SUPA LED EPSRC资助的合作的Stanford和Caltec。预计在项目过程中，预计英国的Photonics KTN的行业 - acad症也有望发展。

项目成果

Interaction of bio-minerals and gels with ultrafast lasers for hard tissue surface engineering

生物矿物质和凝胶与超快激光的相互作用用于硬组织表面工程

• DOI: 10.1109/icton.2015.7193313
• 发表时间: 2015
• 作者: Anastasiou A

Sintering of calcium phosphates with a femtosecond pulsed laser for hard tissue engineering

• DOI: 10.1016/j.matdes.2016.03.159
• 发表时间: 2016-07-05
• 期刊: MATERIALS & DESIGN
• 影响因子: 8.4
• 作者: Anastasiou, A. D.;Thomson, C. L.;Jha, A.
• 通讯作者: Jha, A.

Raman spectroscopy of endoscopic colonic biopsies from patients with ulcerative colitis to identify mucosal inflammation and healing.

• DOI: 10.1364/boe.7.002022
• 发表时间: 2016-05-01
• 期刊: Biomedical optics express
• 影响因子: 3.4
• 作者: Addis J;Mohammed N;Rotimi O;Magee D;Jha A;Subramanian V
• 通讯作者: Subramanian V

Interaction of Femtosecond Pulsed Lasers with Fe2+ and Fe3+ Doped Calcium Phosphates for Bone Tissue Engineering

飞秒脉冲激光与 Fe2 和 Fe3 掺杂磷酸钙的相互作用用于骨组织工程

• DOI: 10.1364/boda.2019.jt4a.36
• 发表时间: 2019
• 作者: Alsubhe E

Laser assisted restorative mineralization of dental enamel

激光辅助牙釉质修复矿化

• DOI: 10.1117/12.2614446
• 发表时间: 2021
• 作者: Anastasiou A