TOPICAL IN-VIVO ELECTRON SPIN RESONANCE

描述：(改编自《摘要》)。潜在的有用性和 局部体内ESR的重要性被引用为以下独一无二的 区别于其他技术的特性： A)体内检测外在顺磁物种(自旋标记) 以生物系统中的特定位置为目标。B)活体监测 使用在微观水平上监控结构顺序的自旋标记 (膜、蛋白质、细胞壁等)C)体内直接检测游离 自由基物种(自然产生的或“捕获”的瞬变产物 自由基反应)。本研究资源的具体目的 发展重建建议包括： 1.低场在体局部ESR的进一步发展和优化 光谱仪，包括低GHz范围内的微波谐振器 (500-1000 MHz)；可行性、灵敏度、电源损耗和 低场局部ESR系统的技术指标。 2.自旋标记在体内的分布和代谢监测 动物： A.氮氧化物代谢和排泄的体内药代动力学 B.通过自旋标记新陈代谢监测肿瘤生长特征 C.猪全心肌缺血的自旋捕获研究 再灌流模型； D.大脑中的氮氧化物监测。

DESCRIPTION: (Adapted from the Abstract). The potential usefulness and importance of topical in-vivo ESR are cited as the following unique properties which distinguish it from other techniques: a) In-vivo detection of extrinsic paramagnetic species (spin labels) targeted to specific sites in a biological system. b) In vivo monitoring using spin labels which monitor structural order at the microscopic level (membranes, proteins, cell walls, etc.) c) Direct in-vivo detection of free radical species (naturally occurring or "trapped" products from transient free radical reactions). The specific aims of this Research Resource development renewal proposal are: 1. Further development and optimization of low field in-vivo topical ESR spectrometers, including microwave resonators in the low GHz range (500-1000 MHz); assessment of feasibility, sensitivity, power losses, and specifications of a low field topical ESR system. 2. In-vivo monitoring of spin label distribution and metabolism in animals: a. in-vivo pharmacokinetics of nitroxide metabolism and excretion b. monitoring tumour growth characteristics by spin label metabolism c. spin trapping studies of a swine global myocardial ischemia reperfusion model; d. nitroxide monitoring in the brain.