Aviv Model 215 Circular Dichroism Sepctrometer

Aviv 215 型圆二色性光谱仪

• 批准号: 6439366
• 负责人: LAWRENCE Jules BERLINER
• 金额: $ 11.61万
• 依托单位: UNIVERSITY OF DENVER (COLORADO SEMINARY)
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6439366
• 关键词: biomedical equipment purchase; dichroism; spectrometry

This is a shared instrumentation request to replace an ailing 22-year old instrument with state-of-the-art capabilities. The high, highly active Core Group faculty have projects covering: calcium binding proteins which fold via the molten globule; preparation and utilization of various myoglobin and carbonic anhydrase mutants and forms to be probed by electron spin resonance methods for intramolecular distance measurements in solution; kinetics and mechanism of folding of a variety of proteins, enzymes and their mutants; and detailed investigations of denatured state energetics. There is a strong institutional emphasis on research as reflected by a 20% cost-sharing commitment and guarantees of maintenance and operating costs throughout the period of this project and beyond. This is a relatively small funding request to the NIH which will certainly be 'value-added' in the ability to generate new and important research from both the NIH funded grantees as well as other user groups generating meaningful results for planning future NIH, federal and other funded research programs. Lastly, as additional 'value- added,' the involvement of collaborative NIH supported researchers at the University of Colorado Health Sciences Center underscores the shared nature of this instrumentation that reaches out beyond the sponsoring institution.

这是一个共享仪器的请求，以取代一个有22年历史的仪器，具有最先进的能力。高，高度活跃的核心组教师的项目包括：钙结合蛋白，通过熔融球折叠;制备和利用各种肌红蛋白和碳酸酐酶突变体和形式，通过电子自旋共振方法探测溶液中的分子内距离测量;各种蛋白质，酶及其突变体的折叠动力学和机制;以及变性状态能量学的详细研究。在整个项目期间及以后，20%的费用分摊承诺和维护和运营费用保证反映了对研究的强烈重视。这对NIH来说是一个相对较小的资金请求，它肯定会“增值”，使NIH资助的受资助者以及其他用户群体能够产生新的重要研究，从而为规划未来的NIH、联邦和其他资助的研究项目产生有意义的结果。最后，作为额外的“附加值”，科罗拉多大学健康科学中心的合作NIH支持的研究人员的参与强调了这种仪器的共享性质，它超越了赞助机构。

项目成果

Proton-mediated dynamics of the alkaline conformational transition of yeast iso-1-cytochrome c.

质子介导的酵母 iso-1-细胞色素 c 碱性构象转变动力学。

• DOI: 10.1021/ja0494454
• 发表时间: 2004
• 期刊: Journal of the American Chemical Society.
• 作者: Martinez,RobertE;Bowler,BruceE
• 通讯作者: Bowler,BruceE

Evaluation of cooperative interactions between substructures of iso-1-cytochrome c using double mutant cycles.

使用双突变体循环评估 iso-1-细胞色素 c 亚结构之间的协同相互作用。

• DOI: 10.1021/bi034958t
• 发表时间: 2003
• 期刊: Biochemistry.
• 作者: Wandschneider,Eydiejo;Hammack,BarbaraN;Bowler,BruceE
• 通讯作者: Bowler,BruceE

Communication of stabilizing energy between substructures of a protein.

蛋白质亚结构之间稳定能量的交流。

• DOI: 10.1021/bi048141r
• 发表时间: 2005
• 期刊: Biochemistry.
• 作者: Kristinsson,Richard;Bowler,BruceE
• 通讯作者: Bowler,BruceE