GUINEA PIG SUBLINGUAL GLAND AS A MUCOUS CELL MODEL

粘蛋白是唾液粘液腺的主要分泌成分， 如大的舌下腺和许多小的唾液腺 腺体 粘蛋白的保护功能 包括：1）口腔表面润滑和水合作用; 2）选择 特定微生物的粘附和清除;以及3） 通过共价结合的或共价结合的膜脂质含量的调节 脂肪酸或通过非共价异型络合。 内 粘液腺，粘液细胞合成和分泌粘蛋白 表型。 研究调节细胞凋亡的分子机制， 粘蛋白的合成和分泌受到细胞内 唾液粘液腺的异质性，这极大地使 细胞特异性分子和细胞内 反应. 一个例外是豚鼠的舌下腺， 完全由粘液腺泡结构组成，因此代表一种 研究粘液细胞生物学重要的潜在模型， 其他污染的腺泡细胞类型。 我们目前正在研究 腺泡结构分泌粘蛋白糖蛋白的调节 通过大鼠舌下腺的酶分散体外衍生。 分散：腺泡结构非常有活力， 对不同的促分泌素有反应，但由两种粘液 细胞和浆液性半月形细胞。 因此，我们计划开发一个类似的 使用豚鼠舌下腺的体外模型，我们计划评估 通过光学显微镜观察细胞模型的形态，以确定细胞 活力和粘液细胞组成。 药理学研究将 以证明存在受监管的 毒蕈碱-胆碱能途径 将使用这些研究的结果 作为正式R 01应用的初步研究， 阐明细胞内信号传导组分的目的 粘液细胞中的通路。

Mucins are the primary secretory component of salivary mucous glands, such as the major sublingual gland and many' of the minor salivary glands. Protective functions which have been attributed to mucins include: 1) lubrication and hydration of oral surfaces; 2) selection for the adherence as well as clearance of -specific micro-organisms; and 3) modulation of the pellicle lipid content through either covalently-bound fatty acids or through non-covalent, heterotypic complexation. Within mucous glands, mucins are synthesized and secreted by the mucous cell phenotype. The study of the molecular mechanisms which regulate the synthesis and secretion of mucins have been hindered by the cellular heterogeneity of salivary mucous glands which greatly complicates the isolation and study of cell-specific molecules and intracellular reactions. An exception is the guinea pig sublingual gland which-is composed entirely of mucous acinar structures and therefore represents an important potential model to study mucous cell biology unencumbered by other contaminating acinar cell types. We currently are studying the regulation of mucin glycoprotein secretion from acinar structures in vitro derived by enzymatic dispersion of rat sublingual glands. Dispersed: acinar structures are extremely viable and pharmacologically responsive to different secretagogues but are composed of both mucous cells and serous demilune cells. Therefore, we plan to develop a similar in vitro model using guinea pig sublingual glands, We plan-to evaluate the cell model morphologically by light microscopy to determine cell viability and mucous cell composition. Pharmacological studies will be conducted to demonstrate the presence of a regulated muscarinic-cholinergic pathway. Results from these studies will be used as preliminary studies for a formal R01 application with the primary objectives of elucidating components of the intracellular signalling pathways in mucous cells.