3-DIMENSIONAL NMR OF DIHYDROFRLATE REDUCTASE
3-DIMENSIONAL NMR OF DIHYDROFRLATE REDUCTASE
批准号:
3426188
负责人:
DAVID H LIVE
金额:
$3.92万
依托单位国家:
美国
项目类别:
财政年份:
1991
资助国家:
美国
项目状态:
已结题
起止时间:
1991-04-15 至 1992-07-31
中文摘要
这项研究工作的目标是加深对
溶液中重要的生物分子以阐明其作用机制
行动的一部分。在这个项目中,提出了一项关于
二氢叶酸还原酶的结构和动力学性质
来自E.Coli。这是一种参与代谢控制的重要酶。
以及生物合成中重要前体的合成。因为这个原因,
它的广泛分布一直是药物设计的目标。几个
抗二氢叶酸还原酶药物已被用作抗菌药物
和抗癌剂。这里的工作将探索药物-酶
相互作用,以改进对此的抑制剂的合理设计
酵素。核磁共振是治疗癌症最有效的技术
这样的研究,但这种蛋白质是对溶液核磁共振的挑战,因为
它的大小。同位素标记的三维核磁共振新技术
蛋白质在溶液构象中表现出很大的前景
分析。这笔拨款将支持一所领先的
正在开发和应用新方法的实验室
促进关于这种酶的方法学的使用。
英文摘要
The goal of the research effort is to develop an understanding of
biologically important molecules in solution to elucidate their mechanism
of action. In this project an investigation is proposed of the
structural and dynamic properties of the enzyme dihydrofolate reductase
from E. coli. This is an important enzyme involved in metabolic control
and synthesis of important precursors in biosynthesis. For this reason,
and its wide distribution it has been a target for drug design. Several
anti-dihydrofolate reductase drugs are already in use as antibacterial
and anticancer agents. The work here will explore the drug - enzyme
interactions to improve the rational design of inhibitors for this
enzyme. Nuclear magnetic resonance is the most effective technique for
such studies, but this protein is a challenge to solution NMR because of
its size. New 3-dimensional NMR techniques with isotopically labeled
protein show a great deal of promise in the solution conformational
analysis. This grant will support research at one of the leading
laboratories where the new approaches are being developed and applied to
facilitate the use of the methodologies on this enzyme.
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会议论文
Structure and Function in alpha-Dystroglycan Glycosylation
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批准号:8898155
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项目类别:
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资助金额:$28.47万
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财政年份:2014
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负责人:DAVID H LIVE
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依托单位:
Structure and Function in alpha-Dystroglycan Glycosylation
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批准号:8767819
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项目类别:
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资助金额:$28.31万
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财政年份:2014
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负责人:DAVID H LIVE
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依托单位:
Structure and Function in alpha-Dystroglycan Glycosylation
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批准号:9906935
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项目类别:
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资助金额:$32.08万
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财政年份:2014
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负责人:DAVID H LIVE
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依托单位:
Acquisition of Microwave-Assisted Automated Peptide Synthesizer
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批准号:7794712
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项目类别:
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资助金额:$14.14万
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财政年份:2010
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负责人:DAVID H LIVE
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依托单位:
Post-Translation Processing of Alpha-Dystroglycan
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批准号:7691724
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项目类别:
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资助金额:$16.23万
-
财政年份:2008
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负责人:DAVID H LIVE
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依托单位:
Post-Translation Processing of Alpha-Dystroglycan
-
批准号:7589523
-
项目类别:
-
资助金额:$19.47万
-
财政年份:2008
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负责人:DAVID H LIVE
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依托单位:
Structural Biology of Cell Surface Mucin Domains
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批准号:6925271
-
项目类别:
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资助金额:$2.47万
-
财政年份:2003
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负责人:DAVID H LIVE
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依托单位:
Structural Biology of Cell Surface Mucin Domains
-
批准号:6943438
-
项目类别:
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资助金额:$25.38万
-
财政年份:2003
-
负责人:DAVID H LIVE
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依托单位:
Structural Biology of Cell Surface Mucin Domains
-
批准号:7117185
-
项目类别:
-
资助金额:$2.45万
-
财政年份:2003
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负责人:DAVID H LIVE
-
依托单位:
Structural Biology of Cell Surface Mucin Domains
-
批准号:6725902
-
项目类别:
-
资助金额:$23.8万
-
财政年份:2003
-
负责人:DAVID H LIVE
-
依托单位:
Structural Biology of Cell Surface Mucin Domains
-
批准号:6802871
-
项目类别:
-
资助金额:$22.99万
-
财政年份:2003
-
负责人:DAVID H LIVE
-
依托单位:
Structural Biology of Cell Surface Mucin Domains
-
批准号:7340077
-
项目类别:
-
资助金额:$21.65万
-
财政年份:2003
-
负责人:DAVID H LIVE
-
依托单位:
CONFORMATION OF MITOGENIC PEPTIDE GROWTH FACTORS
-
批准号:3462781
-
项目类别:
-
资助金额:$5.56万
-
财政年份:1992
-
负责人:DAVID H LIVE
-
依托单位:
CONFORMATION OF MITOGENIC PEPTIDE GROWTH FACTORS
-
批准号:3462779
-
项目类别:
-
资助金额:$5.9万
-
财政年份:1988
-
负责人:DAVID H LIVE
-
依托单位:
CONFORMATION OF MITOGENIC PEPTIDE GROWTH FACTORS
-
批准号:3462780
-
项目类别:
-
资助金额:$9.64万
-
财政年份:1988
-
负责人:DAVID H LIVE
-
依托单位:
COMPUTER MOLECULAR MODELING AND DATA PROCESSING
-
批准号:3520069
-
项目类别:
-
资助金额:$10.8万
-
财政年份:1988
-
负责人:DAVID H LIVE
-
依托单位:
CONFORMATION OF MITOGENIC PEPTIDE GROWTH FACTORS
-
批准号:3462776
-
项目类别:
-
资助金额:$8.58万
-
财政年份:1988
-
负责人:DAVID H LIVE
-
依托单位:
CONFORMATION OF MITOGENIC PEPTIDE GROWTH FACTORS
-
批准号:3462777
-
项目类别:
-
资助金额:$8.87万
-
财政年份:1988
-
负责人:DAVID H LIVE
-
依托单位:
CONFORMATION OF MITOGENIC PEPTIDE GROWTH FACTORS
-
批准号:3462778
-
项目类别:
-
资助金额:$9.06万
-
财政年份:1988
-
负责人:DAVID H LIVE
-
依托单位:
ACQUISTITION OF HIGH FIELD NMR SPECTROMETER
-
批准号:3519828
-
项目类别:
-
资助金额:$30.0万
-
财政年份:1987
-
负责人:DAVID H LIVE
-
依托单位:
海外基金