Acquisition of Microwave-Assisted Automated Peptide Synthesizer

描述（由申请人提供）：申请资金用于购买CEM Liberty全自动微波辅助固相肽合成器和相关配件，以便我们可以将这种先进技术用于各种合成，蛋白质组学和糖蛋白质组学应用，重点是糖生物学。这将加强七名目前NIH资助的研究人员的研究工作，以及一名正在等待NIH申请的新研究人员，一名正在等待美国国家科学基金会资助的研究人员和一名正在等待美国能源部申请的新到来的杰出学者。蛋白质糖基化是蛋白质翻译后加工最普遍和最复杂的模式，是健康和疾病的重要因素，但对糖蛋白的研究往往受到其分子量和聚糖微异质性的限制。合成的糖肽为研究糖蛋白的许多方面提供了强有力的替代试剂。与糖肽合成相关的额外操作严重限制了它们相对于肽的可用性，一个主要限制因素是研究糖蛋白的性质。我们一直在这一领域使用更费力的传统方法，而这一要求的一个关键因素是，新开发的微波辅助方法已被证明特别有利于加速糖肽的合成。微波辅助方法也被证明有利于蛋白水解酶的消化和n -聚糖的释放，解决了一些参与蛋白质组学和糖蛋白组学的研究人员的需要，因此将有助于确定糖蛋白的生物学重要方面，并确定需要合成的相关糖肽以供进一步研究。该仪器将位于乔治亚大学的复杂碳水化合物研究中心，该中心是糖生物学研究的卓越中心，为快速发展的糖生物学领域最佳地利用技术提供了一个独特的环境，包括化学，生物化学，分子生物学和细胞生物学专业知识。

DESCRIPTION (provided by applicant): Funds are requested for the acquisition of a CEM Liberty automated microwave-assisted solid phase peptide synthesizer and associated accessories so that we may employ this advanced technology in a variety of synthesis, proteomic, and glycoproteomic applications, with an emphasis on glycobiology. This will enhance the research efforts of seven currently NIH funded investigators, and a new investigator with a pending NIH application, one with NSF funding and a newly arrived Emminent Scholar with a pending DOE application. Protein glycosylation is the most prevalent and complex mode of protein post-translational processing, being an important factor in health and disease, but studying glycoproteins is often limited by their molecular weight and glycan microheterogeneity. Synthetic glycopeptides provide powerful alternative agents for investigating many aspects of glycoproteins. The additional manipulations associated with glycopeptide synthesis have severely limited their availability relative to peptides, a factor that has been a major limitation is studying properties of glycoproteins. We have been working with more laborious conventional capabilities in this area, and a key factor in this request is the demonstration that the newly developed microwave-assisted methodology has proven particularly beneficial for accelerating glycopeptide synthesis. Microwave-assisted approaches have also proven beneficial in proteolytic enzyme digestions and N-glycan release, addressing the needs of several of the participating investigators in porteomics and glycoproteiomcs, and will therefore help identify biologically important aspects of glycorpoteins and identify relevant glycopeptides to be synthesized for further study. This instrumentation would be located in the Complex Carbohydrate Research Center at the University of Georgia which is a preeminent center in glycobiology research, and provides a unique environment encompassing chemistry, biochemistry, molecular biology and cell biology expertise for optimally exploiting the technology in the rapidly developing field of glycobiology. PUBLIC HEALTH RELEVANCE: The enhanced microwave-assisted technologies in synthesis and proteomics will be employed to advance current research projects primarily focusing on investigating carbohydrate modified proteins. Protein glycosylation is a very important component of post-translational modification with impacts in health and disease, and this instrumentation will facilitate preparation of glycoprotein fragments and analysis of glycoproteins of biomedical significance.