Acquisition of Microwave-Assisted Automated Peptide Synthesizer

购置微波辅助自动肽合成仪

• 批准号: 7794712
• 负责人: DAVID H LIVE
• 金额: $ 14.14万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-01 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7794712
• 关键词: Address; Area; Biochemistry; Carbohydrates; Cellular biology; Chemistry; Complex; Digestion; Disease; Environment; Freedom; Funding; Funding Applicant; Glycobiology; Glycopeptides; Glycoproteins; Health; Methodology; Molecular Biology; Molecular Weight; Peptide Hydrolases; Peptides; Phase; Polysaccharides; Post-Translational Protein Processing; Preparation; Property; Protein Glycosylation; Proteins; Proteomics; Relative (related person); Research; Research Personnel; Research Project Grants; Solid; Technology; United States National Institutes of Health; Universities; Work; instrumentation; microwave electromagnetic radiation; public health relevance

DESCRIPTION (provided by applicant): Funds are requested for the acquisition of a CEM Liberty automated microwave-assisted solid phase peptide synthesizer and associated accessories so that we may employ this advanced technology in a variety of synthesis, proteomic, and glycoproteomic applications, with an emphasis on glycobiology. This will enhance the research efforts of seven currently NIH funded investigators, and a new investigator with a pending NIH application, one with NSF funding and a newly arrived Emminent Scholar with a pending DOE application. Protein glycosylation is the most prevalent and complex mode of protein post-translational processing, being an important factor in health and disease, but studying glycoproteins is often limited by their molecular weight and glycan microheterogeneity. Synthetic glycopeptides provide powerful alternative agents for investigating many aspects of glycoproteins. The additional manipulations associated with glycopeptide synthesis have severely limited their availability relative to peptides, a factor that has been a major limitation is studying properties of glycoproteins. We have been working with more laborious conventional capabilities in this area, and a key factor in this request is the demonstration that the newly developed microwave-assisted methodology has proven particularly beneficial for accelerating glycopeptide synthesis. Microwave-assisted approaches have also proven beneficial in proteolytic enzyme digestions and N-glycan release, addressing the needs of several of the participating investigators in porteomics and glycoproteiomcs, and will therefore help identify biologically important aspects of glycorpoteins and identify relevant glycopeptides to be synthesized for further study. This instrumentation would be located in the Complex Carbohydrate Research Center at the University of Georgia which is a preeminent center in glycobiology research, and provides a unique environment encompassing chemistry, biochemistry, molecular biology and cell biology expertise for optimally exploiting the technology in the rapidly developing field of glycobiology. PUBLIC HEALTH RELEVANCE: The enhanced microwave-assisted technologies in synthesis and proteomics will be employed to advance current research projects primarily focusing on investigating carbohydrate modified proteins. Protein glycosylation is a very important component of post-translational modification with impacts in health and disease, and this instrumentation will facilitate preparation of glycoprotein fragments and analysis of glycoproteins of biomedical significance.

描述（由申请人提供）：资金用于购买CEM Liberty自动微波辅助固相肽合成仪和相关配件，以便我们可以在各种合成，蛋白质组学和糖蛋白质组学应用中采用这种先进技术，重点是糖生物学。这将加强七名目前由NIH资助的研究人员的研究工作，一名新的研究人员正在等待NIH的申请，一名获得NSF资助，一名新来的杰出学者正在等待DOE的申请。蛋白质糖基化是蛋白质翻译后加工中最普遍和最复杂的模式，是健康和疾病的重要因素，但糖蛋白的研究往往受到其分子量和聚糖微异质性的限制。合成糖肽为研究糖蛋白的许多方面提供了强有力的替代试剂。与糖肽合成相关的额外操作严重限制了其相对于肽的可用性，这是研究糖蛋白性质的主要限制因素。我们一直在这一领域使用更费力的常规能力，这一要求的一个关键因素是证明新开发的微波辅助方法已被证明对加速糖肽合成特别有益。微波辅助的方法也被证明有益于蛋白水解酶的二聚体和N-聚糖的释放，解决了参与porteomics和glycoproteiomcs研究的几个研究者的需求，因此将有助于确定生物学重要方面的糖蛋白，并确定相关的糖肽合成进一步研究。该仪器将位于格鲁吉亚大学的复杂碳水化合物研究中心，该中心是糖生物学研究的杰出中心，并提供了一个独特的环境，涵盖化学、生物化学、分子生物学和细胞生物学专业知识，以最佳方式利用该技术在快速发展的糖生物学领域。 公共卫生关系：在合成和蛋白质组学中增强的微波辅助技术将用于推进目前的研究项目，主要集中在研究碳水化合物修饰的蛋白质。蛋白质糖基化是翻译后修饰的一个非常重要的组成部分，对健康和疾病有影响，这种仪器将有助于制备糖蛋白片段和分析具有生物医学意义的糖蛋白。