TREPONEMAL INTERACTIONS WITH EPITHELIAL CELL MONOLAYERS

密螺旋体与上皮细胞单层的相互作用

• 批准号: 3425581
• 负责人: LAWRENCE E WOLINSKY
• 金额: $ 2.22万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-04-01 至 1993-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3425581
• 关键词: Treponema; Treponema infection; biopsy; connective tissue; dental plaque; electron microscopy; epithelium; gingiva; gingival sulcus; host organism interaction; human subject; human tissue; immunoperoxidase; intercellular connection; oral bacteria; pathologic process; periodontitis; radiotracer; statistics /biometry; tissue /cell culture

Microscopic studies of gingival biopsies from humans with advanced adult periodontal disease have shown the presence of oral spirochetes within the periodontal tissues (Frank 1980, Saglie 1982, Wolinsky 1985). It has been suggested that these and other periodontal pathogens originate from within the periodontal pocket and their movement through the gingival crevicular epithelium represents a key event in the progression of destructive periodontal disease. Presently, little information is known about the cellular events leading up to bacterial penetration. It is the specific aim of this investigation to test the hypothesis that oral treponemes infiltrate the gingival tissues through the initial attachment to the gingival sulcular epithelium followed by alteration in the epithelial cell layer eventually leading to the movement of the treponemes through the intercellular epithelial junctions. To test this hypothesis, the attachment, penetration, and movement of radiolabeled treponema sp. through primary cultures of human gingival epithelial cells will be studied. The hypothesis predicts that oral treponemes are able to infiltrate the underlying gingival connective tissues in a highly specific fashion. The resultant weakening of the epithelium structure following treponemal interaction may promote the active or passive ingression of other periodontal pathogenic bacteria. Infiltration of the epithelium by oral Treponemes may be one of many signals which hallmark the onset of active periodontal disease. The results of this study will be used to develop a useful in vitro tissue culture system for studying bacterial interaction with host tissues. The data from this investigation will form the foundation for a larger study. We believe the results of this study will help expand our understanding of the progression of periodontal disease.

晚期成人牙龈活检的显微研究 牙周病表明口腔内存在螺旋体 牙周组织（Frank 1980，Saglie 1982，Wolinsky 1985）。 它有 有人认为这些和其他牙周病原体起源于 牙周袋内及其通过牙龈的运动 裂隙上皮是疾病进展过程中的一个关键事件 破坏性牙周病。 目前所知的信息还很少 关于导致细菌渗透的细胞事件。 它是 这项调查的具体目的是检验以下假设：口腔 密螺旋体通过初始附着侵入牙龈组织 到牙龈沟上皮，然后改变 上皮细胞层最终导致上皮细胞的运动 密螺旋体通过细胞间上皮连接。 为了测试这个 假设，放射性标记的附着、渗透和运动 密螺旋体属通过人牙龈上皮细胞的原代培养 将被研究。 该假设预测口腔密螺旋体能够 以高度渗透到下面的牙龈结缔组织 具体的时尚。 由此导致的上皮结构减弱 密螺旋体相互作用后可能会促进主动或被动 其他牙周病原菌的侵入。 渗透 口腔密螺旋体的上皮细胞可能是标志性的众多信号之一 活动性牙周病的发作。 这项研究的结果将 用于开发有用的体外组织培养系统以进行研究 细菌与宿主组织的相互作用。 数据来自于此 调查将为更大规模的研究奠定基础。 我们相信 这项研究的结果将有助于扩大我们对 牙周病的进展。