TREPONEMAL INTERACTIONS WITH EPITHELIAL CELL MONOLAYERS

晚期成人牙周炎患者牙龈活检的显微镜观察 牙周病已经表明口腔螺旋体的存在， 牙周组织（Frank 1980，Saglie 1982，Wolinsky 1985）。 它有 有人认为这些和其他牙周病原体来源于 以及它们通过牙龈的运动 龈沟上皮代表了一个关键事件的进展， 破坏性牙周病。 目前，我们所知甚少 关于导致细菌侵入的细胞活动。 是 这项调查的具体目的是测试假设， 密螺旋体通过最初的附着物渗入牙龈组织 牙龈沟上皮，然后改变 上皮细胞层最终导致运动的 密螺旋体通过细胞间上皮连接。 为了验证这一 假设，附着，渗透，和移动放射性标记的 通过人牙龈上皮细胞的原代培养来检测密螺旋体 将被研究。 该假说预测，口腔密螺旋体能够 以高度渗透的方式渗入下面的牙龈结缔组织 具体的时尚。 上皮结构的弱化 密螺旋体相互作用后， 其他牙周致病菌的侵入。 浸润 口腔密螺旋体上皮细胞可能是许多标志性信号之一， 活动性牙周病的发病。 这项研究的结果将 建立了一套实用的离体组织培养体系， 细菌与宿主组织的相互作用。 从这个数据 调查将为更大规模的研究奠定基础。 我们认为 这项研究的结果将有助于扩大我们对 牙周病的危害

Microscopic studies of gingival biopsies from humans with advanced adult periodontal disease have shown the presence of oral spirochetes within the periodontal tissues (Frank 1980, Saglie 1982, Wolinsky 1985). It has been suggested that these and other periodontal pathogens originate from within the periodontal pocket and their movement through the gingival crevicular epithelium represents a key event in the progression of destructive periodontal disease. Presently, little information is known about the cellular events leading up to bacterial penetration. It is the specific aim of this investigation to test the hypothesis that oral treponemes infiltrate the gingival tissues through the initial attachment to the gingival sulcular epithelium followed by alteration in the epithelial cell layer eventually leading to the movement of the treponemes through the intercellular epithelial junctions. To test this hypothesis, the attachment, penetration, and movement of radiolabeled treponema sp. through primary cultures of human gingival epithelial cells will be studied. The hypothesis predicts that oral treponemes are able to infiltrate the underlying gingival connective tissues in a highly specific fashion. The resultant weakening of the epithelium structure following treponemal interaction may promote the active or passive ingression of other periodontal pathogenic bacteria. Infiltration of the epithelium by oral Treponemes may be one of many signals which hallmark the onset of active periodontal disease. The results of this study will be used to develop a useful in vitro tissue culture system for studying bacterial interaction with host tissues. The data from this investigation will form the foundation for a larger study. We believe the results of this study will help expand our understanding of the progression of periodontal disease.