NMR ANALYSIS OF BONDING INTERACTIONS OF DENTIN ADHESIVES

牙本质粘合剂粘合相互作用的核磁共振分析

• 批准号: 3425623
• 负责人: LAWRENCE E WOLINSKY
• 金额: $ 3.74万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-06-01 至 1994-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3425623
• 关键词: adult human (21+); aminoacid analog; carbon; chemical association; chemical bond resonance; chemical synthesis; collagen; dental adhesive /sealant; dentin; glycine; human tissue; hydrogen bond; hydroxyapatites; ionic bond; nuclear magnetic resonance spectroscopy; physical chemical interaction; polymethacrylate; solid state; solutions; stable isotope; tooth surface

During the past decade advances in dental science have led to the introduction of many improved restorative materials which offer long-term stability in the oral cavity. In the area of dental adhesives much interest has focused upon the development of agents capable of chemically bonding with tooth structure. There is an extensive body of knowledge available on the physical characteristics of the interface between these adhesive materials and the tooth surface. However, very little is presently known about the chemical nature of this interface, in particular, what occurs on a molecular level between the organic adhesive agents and the mineralized tooth substrata. The purpose of this study is to utilize solid-state carbon-13 nuclear magnetic resonance (13C-NMR) to elucidate the chemical nature of the bonding interactions of the two adhesive agents N-phenyl glycine (NPG) and its gylcidyl methyl methacrylate analogue (NPG-GMA) with human dentin. Carbon-13 iostope-enriched NPG and NPG-GMA will be synthesized and interacted with prepared human dentin and dentinal collagen. The treated dentinal components will be subjected to high resolution 13C-NMR using a cross-polarization-magic angle spinning method. The nature of chemical bonding interactions between these agents and the dentin support will be determined from the resonance shifts seen in the 13C-NMR. We hypothesize that increased chemical bonding interactions between the bonding agent and the dentin will result in observable downfield anisotropic shifts of the carbon-13 labeled agent which will be seen in the 13C-NMR. The successful application of solid-state NMR technology to study the chemical interactions of dental adhesives will serve as the basis for a larger proposal to investigate bonding interactions among other families of bonding agents and to study the stability of these bonding materials under a variety of biological environments. The long-term goal of this proposal is to develop the application of "state of the art" NMR technology to reliably predict microleakage potential and the adhesive characteristics of a dental materials. Solid-state NMR offers the potential to quantitatively measure traditional parameters of adhesion with refined technologies. These carbon-13 enriched probes in conjunction with this advanced NMR analytical technology will allow the study of the molecular chemistry of the bonding interface at the restorative-tooth junction.

在过去的十年里，牙科科学的进步导致了 介绍多种可长期修复的改良型修复材料 口腔内的稳定性。在牙科粘合剂领域 人们的兴趣主要集中在能够在化学上 采用齿形结构粘接。有广泛的知识体系 有关这些接口之间的物理特性的信息 粘合材料和牙齿表面。然而，很少有 目前已知该界面的化学性质，特别是， 有机粘合剂和有机粘合剂之间在分子水平上发生了什么 矿化的牙齿基质。这项研究的目的是利用 固体碳-13核磁共振(13C-核磁共振)研究 两种胶粘剂粘接作用的化学性质 N-苯基甘氨酸(NPG)及其甲基丙烯酸缩水甘油酯类似物 (NPG-GMA)与人牙本质。富碳~(13)碘的NPG和NPG-GMA 将被合成并与准备好的人牙本质和牙本质相互作用 胶原蛋白。经过处理的牙本质部件将受到较高的 使用交叉极化魔角旋转方法解析13C-核磁共振。 这些试剂与分子间化学键相互作用的性质 牙本质的支持将根据在 13C-核磁共振谱。我们假设增加化学键的相互作用 粘结剂和牙本质之间的关系会导致观察到 碳-13标记试剂的下场各向异性位移 在~(13)C-核磁共振中可以看到。固体核磁共振的成功应用 研究牙科粘合剂的化学相互作用的技术将 作为一个更大的研究结合的方案的基础 其他粘结剂家族之间的相互作用和研究 这些粘结材料在不同生物环境下的稳定性 环境。这项建议的长期目标是发展 现代核磁共振技术在可靠预测中的应用 牙体微渗漏电位与粘附性的关系 材料。固态核磁共振提供了定量测量的可能性 传统的粘接参数与精细化的技术。这些 碳-13富集型探针与这一先进的核磁共振分析相结合 技术将允许研究键合的分子化学。 修复体-牙齿连接处的界面。