NMR ANALYSIS OF BONDING INTERACTIONS OF DENTIN ADHESIVES

在过去的十年中，牙科科学的进步导致了 采用许多改进的修复材料， 口腔内的稳定性。 在牙科粘合剂领域， 人们的兴趣集中在开发能够化学地 与牙齿结构结合。 有大量的知识 可根据这些之间的界面的物理特性 粘合剂材料和牙齿表面。 然而，很少有 目前已知这种界面的化学性质，特别是， 在有机粘合剂之间的分子水平上发生了什么， 矿化的牙齿基质 本研究的目的是利用 固态碳-13核磁共振（13 C-NMR）以阐明 两种粘合剂的粘合相互作用的化学性质 N-苯基甘氨酸及其甲基丙烯酸甲酯类似物 （NPG-GMA）与人牙本质的接触。 碳-13同位素富集的NPG和NPG-GMA 将合成并与制备的人牙本质和牙本质 胶原 经处理的牙本质组分将经受高强度的热处理。 使用交叉极化-魔角旋转方法的13 C-NMR分辨率。 这些试剂和药物之间的化学键合相互作用的性质 牙本质支撑将从共振位移中确定， 13碳核磁共振。 我们假设增加的化学键相互作用 粘合剂和牙本质之间的接触将导致可观察到的 碳-13标记试剂的低场各向异性位移， 在13 C-NMR中可以看到。 固体核磁共振的成功应用 研究牙科粘合剂的化学相互作用的技术将 作为研究键合的更大提案的基础 其他家庭的粘合剂之间的相互作用，并研究 这些粘合材料在各种生物条件下的稳定性 环境. 这项建议的长远目标是发展 应用“最先进的”NMR技术可靠地预测 微渗漏的可能性和牙科的粘附特性 材料. 固态核磁共振提供了定量测量的潜力 传统的粘附参数与精细的技术。 这些 碳-13富集探针与这种先进的核磁共振分析技术相结合， 技术将允许研究键合的分子化学， 在牙-牙接合处的界面。

During the past decade advances in dental science have led to the introduction of many improved restorative materials which offer long-term stability in the oral cavity. In the area of dental adhesives much interest has focused upon the development of agents capable of chemically bonding with tooth structure. There is an extensive body of knowledge available on the physical characteristics of the interface between these adhesive materials and the tooth surface. However, very little is presently known about the chemical nature of this interface, in particular, what occurs on a molecular level between the organic adhesive agents and the mineralized tooth substrata. The purpose of this study is to utilize solid-state carbon-13 nuclear magnetic resonance (13C-NMR) to elucidate the chemical nature of the bonding interactions of the two adhesive agents N-phenyl glycine (NPG) and its gylcidyl methyl methacrylate analogue (NPG-GMA) with human dentin. Carbon-13 iostope-enriched NPG and NPG-GMA will be synthesized and interacted with prepared human dentin and dentinal collagen. The treated dentinal components will be subjected to high resolution 13C-NMR using a cross-polarization-magic angle spinning method. The nature of chemical bonding interactions between these agents and the dentin support will be determined from the resonance shifts seen in the 13C-NMR. We hypothesize that increased chemical bonding interactions between the bonding agent and the dentin will result in observable downfield anisotropic shifts of the carbon-13 labeled agent which will be seen in the 13C-NMR. The successful application of solid-state NMR technology to study the chemical interactions of dental adhesives will serve as the basis for a larger proposal to investigate bonding interactions among other families of bonding agents and to study the stability of these bonding materials under a variety of biological environments. The long-term goal of this proposal is to develop the application of "state of the art" NMR technology to reliably predict microleakage potential and the adhesive characteristics of a dental materials. Solid-state NMR offers the potential to quantitatively measure traditional parameters of adhesion with refined technologies. These carbon-13 enriched probes in conjunction with this advanced NMR analytical technology will allow the study of the molecular chemistry of the bonding interface at the restorative-tooth junction.