CRYO-ANALYTIC ELECTRON MICROSCOPY IN CHICK EMBRYOS

本研究的长期目标是：（a）编制一份清单 各种细胞和组织中的可扩散离子， 鸡早期关键发育过程中的成分 （B）将该信息与其他已知的生物化学信息相关联 数据，以及（c）解释这些数据，因为它们与发展有关 事件 具体目标包括：（a）改进速冻方法， 冷冻切片和分析研究;（B）测量可扩散离子 细胞、组织和细胞外隔室中的浓度 发育中的鸡胚，以及（c）将 胚胎的各个隔室具有显著的细胞变化， 形态发生事件 实现这些目标将提供重要的 信息在理解正常发展和发展中的作用 受到各种实验操作、致畸剂的干扰，或 衰老 将鸡胚用冷冻剂进行速冻， 这避免了可扩散离子的移动。 切片 冷冻干燥，观察并在分析仪中进行元素分析 电镜 将积累定性和定量数据 在各种细胞和胚胎隔室中， 根据发生的已知发育事件进行分析和解释 在时间和空间上。

The long term objectives of this research are to (a) develop an inventory of diffusible ions in various cells and tissues which represent important components involved in critical developmental processes in early chick development, (b) to correlate this information with other known biochemical data, and (c) to interpret these data as they relate to developmental events. The specific aims include: (a) to improve quick-freezing methods for cryosectioning and analytical studies; (b) to survey diffusible ion concentrations in cells, tissues and extra-cellular compartments of the developing chick embryo, and (c) to correlate the ion concentrations in various compartments of the embryo with significant cellular changes and morphogenetic events. Acheivement of these aims will provide important information in the understanding of normal development and in development perturbated by various experimental manipulations, teratogenci agents, or aging. Chick embryos will be quick-frozen by cryogens and cryosectioned by methods which avoid the mobilization of diffusible ions. Sections will be freeze-dried, viewed and subjected to elemental analysis in an analytical electron microscope. Qualitative and quantitative data will be accumulated in various cellular and embryonic compartments, subjected to computer analysis and interpreted in light of known developmental events occurring in time and space.