CRYO-ANALYTIC ELECTRON MICROSCOPY IN CHICK EMBRYOS

这项研究的长期目标是(A)制定一份清单 各种细胞和组织中的可扩散离子，它们代表着重要的 雏鸡早期关键发育过程中的组成成分 发展，(B)将这一信息与其他已知的生化信息相关联 数据，以及(C)解释这些数据，因为它们与发展 事件。 具体目标包括：(A)改进速冻方法 冰冻切片和分析研究；(B)测量可扩散离子 在细胞、组织和胞外隔间的浓度 和(C)与鸡胚胎中离子浓度的相关性。 胚胎的各个隔室有显著的细胞变化和 形态发生事件。这些目标的实现将提供重要的 理解正常发展和发展中的信息 受到各种实验操作的干扰，致畸剂或 衰老。 鸡胚胎将用冷冻剂快速冷冻，并通过各种方法进行冷冻 这避免了可扩散离子的动员。各节将是 冷冻干燥，观察并在分析中进行元素分析 电子显微镜。将积累定性和定量的数据 在各种细胞和胚胎的隔间中，受制于计算机 根据已知的发展事件进行分析和解释 在时间和空间上。

The long term objectives of this research are to (a) develop an inventory of diffusible ions in various cells and tissues which represent important components involved in critical developmental processes in early chick development, (b) to correlate this information with other known biochemical data, and (c) to interpret these data as they relate to developmental events. The specific aims include: (a) to improve quick-freezing methods for cryosectioning and analytical studies; (b) to survey diffusible ion concentrations in cells, tissues and extra-cellular compartments of the developing chick embryo, and (c) to correlate the ion concentrations in various compartments of the embryo with significant cellular changes and morphogenetic events. Acheivement of these aims will provide important information in the understanding of normal development and in development perturbated by various experimental manipulations, teratogenci agents, or aging. Chick embryos will be quick-frozen by cryogens and cryosectioned by methods which avoid the mobilization of diffusible ions. Sections will be freeze-dried, viewed and subjected to elemental analysis in an analytical electron microscope. Qualitative and quantitative data will be accumulated in various cellular and embryonic compartments, subjected to computer analysis and interpreted in light of known developmental events occurring in time and space.