MEETING: GH PITUITARY CELLS IN MOLECULAR & CELL BIOLOGY

GH细胞是大鼠垂体细胞的克隆株，可以连续培养 在文化中无限期地传播，并表现出许多 正常生长细胞和乳汁滋养细胞在原位的分化功能 换句话说，它们分泌真正的大鼠生长激素和催乳素，以及 对控制生物合成的许多调控信号做出反应，并 分泌这两种蛋白质荷尔蒙。因为它们很容易生长， 现成的可获得性、高的特异性激素产生和反应率 对调节因子来说，这些细胞株已广泛应用于 在过去的几年里，机械研究不能在 异质原代培养，在整个脑垂体腺中，或在完整的 动物。这些调查在 分子生物学领域(分离、表征和调节 生长激素和催乳素基因)、细胞生物学(激素的超微结构研究 包装和分泌；激素受体特性和 转导；肌醇磷脂代谢、细胞内钙离子和 调节生长激素和催乳素合成和释放的蛋白质磷酸化)， 和生物物理学(分泌细胞的电生理学，电压依赖和 不依赖于电压的钙通道)。在过去的一年， 分子生物学、细胞生物学和细胞生物学之间的相互关系 生长激素细胞的神经生物学已经变得清晰，但领先的研究人员 在不同的地区从未聚集在一个共同的地点分享他们的 最新的见解和知识。这样做的目的是 因此，研讨会/会议是为了汇聚一群最 从事生长激素细胞研究的活跃科学家和几位主要的 主要相关学科的研究人员交流知识 他们的最新发现和技术，并使当代 用于解决几个主要未解决的分子的方法学 生理学问题(如特定基因的荷尔蒙调节 细胞内钙离子在蛋白质分泌中的表达、作用及配体 对特定离子通道的调节)。

GH cells are clonal strains of rat pituitary cells that can be serially propagated in culture indefinitely and that perform many of the differentiated functions of normal somatotrophs and lactotrophs in situ, in other words, they secrete authentic rat growth hormone and prolactin and respond to many of the regulatory signals that control the biosynthesis and secretion of these two protein hormones. Because of their ease of growth, ready availability, high rates of specific hormone production and response to regulatory factors, these cell strains have become widely used in the last several years for mechanistic studies which could not be performed in heterogeneous primary cultures, in whole pituitary glands, or in intact animals. These investigations have been particularly informative in the areas of molecular biology (isolation, characterization and regulation of the genes for GH and PRL), cell biology (ultrastructural studies on hormone packaging and secretion; hormone receptor characterization and transduction; roles of phosphoinositide metabolism, intracellular Ca2+, and protein phosphorylation in regulation GH and PRL synthesis and release), and biophysics (electrophysiology of secretory cells, voltage-dependent and voltage-independent Ca2+ channels). In the last year, the interrelationships between the molecular biology, cell biology, and neurobiology of GH cells has become clear, and yet the leading researchers in the separate areas have never convened at a common site to share their most current insights and knowledge. The purpose of this Workshop/Conference is, therefore, to bring together a group of the most active scientists working on GH cells and several of the premier researchers in the major relevant disciplines to exchange knowledge of their latest findings and techniques, and to enable contemporary methodology to be used to solve several of the principal unsolved molecular problems in physiology (e.g. hormonal regulation of specific gene expression, role of intracellular Ca2+ in protein secretion, and ligand regulation of specific ion channels).