CHORDA-LINGUAL NERVE INJURY AND CHEMOSENSORY PERFORMANCE

脊索-舌神经损伤和化学感觉性能

• 批准号: 3425673
• 负责人: JOHN R ZUNIGA
• 金额: $ 3.77万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-30 至 1994-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3425673
• 关键词: atrophy; chemoreceptors; citrates; denervation; human subject; injury; innervation; microscopy; oral pharyngeal surgery; postoperative complications; sensory discrimination; sensory receptors; taste; taste buds; taste threshold; tongue; videotape /videodisc; visceral afferent nerve

Description: (Adapted From The Applicant's Abstract) The objective of this project is to evaluate the impact of severe chorda-lingual nerve injury on gustation and taste bud density, specifically the capacity of human subjects with unilateral nerve injury to detect and scale the strength of different concentrations of citrate applied to spatially- matched, isolated, discrete regions of the anterior tongue tip. The anatomical characteristics of fungiform taste buds within the same site tested for citrate taste perception will be investigated using a non- destructive staining method and videomicroscopy. It is hypothesized that severe injury to the chorda-lingual nerve in humans results in the loss or depletion of taste sensibility due in part to denervation atrophy of fungiform taste buds. Information from these studies will provide a method to diagnose hypogeusias following injury to the chorda- lingual innervation in humans and should thus contribute to the understanding and treatment of taste disorders which involve the chorda- lingual nerve. Citrate detection thresholds and subjective strengths of taste intensity will be assessed in healthy men and women with unilateral severe chorda- lingual nerve injury as a result of dental or maxillofacial surgery. A two-alternative forced choice, modified staircase procedure will be used to derive threshold. Cross-modal magnitude matching functions for taste intensity judgement will be obtained for five concentrations of citrate, and measures of perceived strength will de determined. The right and left tongue tip surface will be tested to assess the effect of selective nerve damage on citrate taste perception. Citrate solutions will be delivered to an enclosed, self-retained chamber reinforced to the surface of the right/left tongue tip so that only a discrete and isolated surface area of the tongue will be tested. The taste buds bathed by stimuli within the chamber will be distinguished by methylene blue stain and recorded by videomicroscopy. The number of fungiform papillae and pores per unit area will be determined so that the relationship of discriminative taste performance for a known number of taste buds can be examined. Comparisons of global (i.e., whole mouth) threshold and suprathreshold scales of citrate taste intensity will be made to elucidate the effect of unilateral nerve injury on global taste perception.

描述：（改编自申请人的摘要） 本项目旨在评估严重的脊索舌神经损伤对 对味觉和味蕾密度的伤害，特别是 人类受试者与单侧神经损伤，以检测和衡量 不同浓度的柠檬酸盐应用于空间- 匹配的、孤立的、离散的前舌尖区域。 的 同一部位菌状味蕾的解剖学特征 测试柠檬酸盐味觉将使用非 破坏性染色法和视频显微镜。 据推测 严重损伤人类的舌索神经会导致 部分由于去神经支配而丧失或耗尽味觉 菌状味蕾萎缩。 这些研究的信息将 提供了一种诊断脊索损伤后味觉减退的方法， 人类的舌神经支配，因此应该有助于 理解和治疗涉及脊索的味觉障碍， 舌神经 柠檬酸检测阈值和味觉强度的主观强度 将在单侧严重脊索的健康男性和女性中进行评估， 由于牙科或颌面外科手术造成的舌神经损伤。 一 二选一强制选择，采用改良阶梯程序 来推导阈值。 用于味觉的跨模态幅度匹配函数 将获得五种浓度柠檬酸盐的强度判断， 并且将确定感知强度的测量。 方面的权利和 左舌尖表面将被测试，以评估选择性 柠檬酸盐味觉神经损伤。 柠檬酸盐溶液将 被送到一个封闭的，自我保留的房间， 右/左舌尖的表面，使得仅分离的且 将测试舌的隔离表面区域。 味蕾 通过室内的刺激沐浴将由亚甲基区分 蓝色染色并通过视频显微镜记录。 真菌数量 将确定每单位面积的乳头和孔， 对于已知数量的 可以检查味蕾。 全局比较（即，全口） 柠檬酸盐味觉强度的阈值和阈上量表将 阐明单侧神经损伤对整体味觉的影响 perception.