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REGENERATION AFTER MANDIBULAR NERVE SECTION & REPAIR

下颌神经切断后的再生

基本信息

批准号：
3425254
负责人：
JOHN R ZUNIGA
金额：
$ 2.14万
依托单位：
UNIV OF NORTH CAROLINA CHAPEL HILL
依托单位国家：
美国
项目类别：
财政年份：
1987
资助国家：
美国
起止时间：
1987-09-01 至 1989-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3425254
关键词：
ganglion cell nervous system regeneration neurosurgery neurotransmitters peripheral nervous system

项目摘要

The hypothesis that guides this project is that trigeminal nerve axotomy results in: (i) the degeneration or atrophy of selective trigeminal ganglion cell populations, and (ii) transganglionic degeneration of second-order neurons within the spinal trigeminal and main sensory nucleus. Furthermore, repair by epineurial reanastomosis results in: (i) regeneration of selective trigeminal ganglion cell populations, and (ii) enhances the process of transganglionic regeneration that normally follows central degeneration within the spinal trigeminal and main sensory nucleus. The proposed studies seek to carefully evaluate the anatomical events of trigeminal axotomy and the consequences of early versus delayed direct repair. In general, we propose to carry out a detailed evaluation of existing cell populations in the trigeminal ganglion after peripheral axotomy and early of delayed (months) repair by using staggered double-fluorescence labeling methods in rats. The presence of double-labeling and the morphometric characteristics of existing trigeminal ganglion cells will be compared with those obtained from control (sham and axotomy-without-repair operated) rats. In a parallel study, the same rats (axotomy with early and delayed repair and controls) will be evaluated for the presence of transganglionic degeneration (using a genuine marker for transganglionic regulation of sensory neurons) within the spinal trigeminal and main sensory nucleus. It is anticipated that the evaluation for double-labeling and morphometric change of ganglion cells, and detailed observance of transganglionic regulation after injury and repair of the trigeminal nerve will: (i) determine if, and to what extent, selective cell degeneration occurs in the ganglion after peripheral axotomy; (ii) more clearly resolve the question of whether nerve repair results in the reconnection of ganglion cells; (iii) characterize the effects of peripheral nerve injury and repair on second-order neurons and, finally (iv) enable finer stratification of guidelines for peripheral nerve repair. The methodologies and anticipated results, if verified by these studies, should provide the foundation for a more expanded project to examine the effects of therapeutic interventions intended to alleviate the pain and discomfort associated with peripheral nerve injury in the orofacial region.

指导这个项目的假设是三叉神经轴突切断导致：（i）选择性轴突的变性或萎缩，三叉神经节细胞群，和（ii）跨神经节的三叉神经脊束内二级神经元变性和主要感觉核。此外，神经外膜修复再吻合导致：（i）选择性三叉神经再生神经节细胞群，和（ii）增强的过程，跨神经节再生，通常遵循中央三叉神经脊束和主要感觉神经内的变性原子核拟议的研究旨在仔细评估三叉神经切断术的解剖学事件及其后果早期与延迟直接修复。总的来说，我们建议对现有的细胞群进行详细的评估，三叉神经节周围神经切断术后和延迟的早期（月）使用交错双荧光标记的修复方法在大鼠。双标记的存在和现有三叉神经节细胞的形态计量学特征将与从对照（假手术和轴突切断术-无修复手术）大鼠。在一项平行研究中，相同的大鼠（轴突切断早期和延迟修复和对照）将评价是否存在跨神经节变性（使用真正的标记物进行感觉神经节的跨神经节调节）三叉神经脊束核和主感觉核内的神经元）。预计双标签和神经节细胞的形态计量学变化，并详细观察的跨神经节调节损伤后和修复的三叉神经将：（i）确定是否，以及在多大程度上，在外周血中，轴突切开术;（ii）更清楚地解决神经是否修复导致神经节细胞的重新连接;（iii）描述周围神经损伤和修复对二阶神经元，最后（iv）使更精细的分层周围神经修复指南。的方法和如果这些研究证实了预期的结果，为一个更大的项目奠定了基础，旨在缓解疼痛的治疗干预措施，与口面周围神经损伤相关的不适地区